scholarly journals MxA transcripts with distinct first exons and modulation of gene expression levels by single-nucleotide polymorphisms in human bronchial epithelial cells

2012 ◽  
Vol 65 (2) ◽  
pp. 107-114 ◽  
Author(s):  
Satoshi Noguchi ◽  
Minako Hijikata ◽  
Emi Hamano ◽  
Ikumi Matsushita ◽  
Hideyuki Ito ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Single Nucleotide Polymorphisms ◽  
Bronchial Epithelial Cells ◽  
Nucleotide Polymorphisms ◽  
Human Bronchial Epithelial Cells ◽  
Single Nucleotide ◽  
Bronchial Epithelial ◽  
Modulation Of Gene Expression ◽  
Gene Expression Levels

scholarly journals Interleukin-8 gene expression in human bronchial epithelial cells.

1991 ◽  
Vol 266 (29) ◽  
pp. 19611-19617
Author(s):  
H. Nakamura ◽  
K. Yoshimura ◽  
H.A. Jaffe ◽  
R.G. Crystal
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Bronchial Epithelial Cells ◽  
Interleukin 8 ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial

Gene expression profiling of human bronchial epithelial cells exposed to seasonal Milan particulate matter

2014 ◽  
Vol 229 ◽  
pp. S71
Author(s):  
Eleonora Longhin ◽  
Laura Capasso ◽  
Cristina Battaglia ◽  
Cristina Cosentino ◽  
Maria Carla Proverbio ◽  
...  
Keyword(s):  
Gene Expression ◽  
Particulate Matter ◽  
Epithelial Cells ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Bronchial Epithelial Cells ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial

Gene expression changes in human bronchial epithelial cells (BEAS-2B) and human pulmonary alveolar epithelial cells (HPAEpiC) after interaction with Cladosporium sphaerospermum

2019 ◽  
Vol 58 (3) ◽  
pp. 333-340 ◽  
Author(s):  
Sing Gee Lo ◽  
Shew Fung Wong ◽  
Joon Wah Mak ◽  
Khi Khi Choo ◽  
Kee Peng Ng
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Germination Rate ◽  
Bronchial Epithelial Cells ◽  
Alveolar Epithelial Cells ◽  
Differentially Expressed ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial ◽  
Alveolar Epithelial ◽  
Cladosporium Sphaerospermum

Abstract Cladosporium is one of the most abundant spore. Fungi of this genus can cause respiratory allergy and intrabronchial lesion. We studied the differential expression of host genes after the interaction of Cladosporium sphaerospermum conidia with Human Bronchial Epithelial Cells (BEAS-2B) and Human Pulmonary Alveolar Epithelial Cells (HPAEpiC). C. sphaerospermum conidia were harvested and co-cultured with BEAS-2B cells or HPAEpiC cells for 48 hours respectively. This culture duration was chosen as it was associated with high germination rate. RNA was extracted from two biological replicates per treatment. RNA of BEAS-2B cells was used to assess changes in gene expression using AffymetrixGeneChip® Human Transcriptome Array 2.0. After co-culture with Cladosporium spores, 68 individual genes were found differentially expressed (P ≤ 0.05) and up-regulated ≥ 1.5 folds while 75 genes were found differentially expressed at ≤ −1.5 folds compared with controls. Reverse transcription and qPCR were performed on the RNA collected from both BEAS-2B cells and HPAEpiC cells to validate the microarray results with 7 genes. Based on the findings, infected pulmonary epithelial cells exhibited an increase in cell death-related genes and genes associated with innate immunity.

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