Rapid screening and dereplication of bacterial isolates from marine sponges of the Sula Ridge by Intact-Cell-MALDI-TOF mass spectrometry (ICM-MS)

The use of cell lines in research can be affected by cell line misidentification. Short tandem repeat (STR) analysis is an effective method, and the gold standard, for the identification of the genetic origin of a cell line, but methods that allow the discrimination between cell lines of the same genetic origin are lacking. Here, we use intact cell MALDI-ToF mass spectrometry analysis, routinely used for the identification of bacteria in clinical diagnostic procedures, for the authentication of a set of cell lines consisting of three parental neuroblastoma cell lines (IMR-5, IMR-32 and UKF-NB-3) and eleven drug-adapted sublines. Principal component analysis (PCA) of intact-cell MALDI-ToF mass spectrometry data revealed clear differences between most, but not all, of the investigated cell lines. Mass spectrometry whole-cell fingerprints enabled the separation of IMR-32 and its clonal subline IMR-5. Sublines that had been adapted to closely related drugs, for example, the cisplatin- and oxaliplatin-resistant UKF-NB-3 sublines and the vincristine- and vinblastine-adapted IMR-5 sublines, also displayed clearly distinctive patterns. In conclusion, intact whole-cell MALDI-ToF mass spectrometry has the potential to be further developed into an authentication method for mammalian cells of a common genetic origin.

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Characterization of fungal antagonistic bacilli isolated from aerial roots of banyan (Ficus benghalensis ) using intact-cell MALDI-TOF mass spectrometry (ICMS)

Journal of Applied Microbiology ◽

10.1111/jam.12161 ◽

2013 ◽

Vol 114 (5) ◽

pp. 1300-1310 ◽

Cited By ~ 14

Author(s):

K.V. Pathak ◽

H. Keharia

Keyword(s):

Mass Spectrometry ◽

Intact Cell ◽

Maldi Tof Mass Spectrometry ◽

Maldi Tof ◽

Ficus Benghalensis ◽

Aerial Roots

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Intact cell MALDI-TOF mass spectrometry, a promising proteomic profiling method in farm animal clinical and reproduction research

Theriogenology ◽

10.1016/j.theriogenology.2020.02.037 ◽

2020 ◽

Vol 150 ◽

pp. 113-121

Author(s):

Laura Soler ◽

Svetlana Uzbekova ◽

Elisabeth Blesbois ◽

Xavier Druart ◽

Valérie Labas

Keyword(s):

Mass Spectrometry ◽

Intact Cell ◽

Farm Animal ◽

Proteomic Profiling ◽

Maldi Tof Mass Spectrometry ◽

Maldi Tof

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Rapid Screening of Epidemiologically Important Salmonella enterica subsp. enterica Serovars by Whole-Cell Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Applied and Environmental Microbiology ◽

10.1128/aem.02418-10 ◽

2011 ◽

Vol 77 (12) ◽

pp. 4136-4146 ◽

Cited By ~ 95

Author(s):

Ralf Dieckmann ◽

Burkhard Malorny

Keyword(s):

Mass Spectrometry ◽

Salmonella Enterica ◽

Laser Desorption ◽

Rapid Screening ◽

Laser Desorption Ionization ◽

Ionization Time ◽

Content Type ◽

Maldi Tof Mass Spectrometry ◽

Maldi Tof ◽

Matrix Assisted Laser

ABSTRACTCurrently, 2,610 differentSalmonellaserovars have been described according to the White-Kauffmann-Le Minor scheme. They are routinely differentiated by serotyping, which is based on the antigenic variability at lipopolysaccharide moieties (O antigens), flagellar proteins (H1 and H2 antigens), and capsular polysaccharides (Vi antigens). The aim of this study was to evaluate the potential of matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry for rapid screening and identification of epidemiologically importantSalmonella entericasubsp.entericaserovars based on specific sets of serovar-identifying biomarker ions. By analyzing 913Salmonella entericasubsp.entericastrains representing 89 different serovars using MALDI-TOF mass spectrometry, several potentially serovar-identifying biomarker ions were selected. Based on a combination of genus-, species-, subspecies-, and serovar-identifying biomarker ions, a decision tree classification algorithm was derived for the rapid identification of the five most frequently isolatedSalmonella entericaserovars, Enteritidis, Typhimurium/4,[5],12:i:-, Virchow, Infantis, and Hadar. Additionally, sets of potentially serovar-identifying biomarker ions were detected for other epidemiologically interesting serovars, such as Choleraesuis, Heidelberg, and Gallinarum. Furthermore, by using a bioinformatic approach, sequence variations corresponding to single or multiple amino acid exchanges in several biomarker proteins were tentatively assigned. The inclusivity and exclusivity of the specific sets of serovar-identifying biomarker ions for the top 5 serovars were almost 100%. This study shows that whole-cell MALDI-TOF mass spectrometry can be a rapid method for prescreeningS. entericasubsp.entericaisolates to identify epidemiologically important serovars and to reduce sample numbers that have to be subsequently analyzed using conventional serotyping by slide agglutination techniques.

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