Intact cell MALDI-TOF mass spectrometry-based approaches for the diagnosis of bloodstream infections

2011 ◽  
Vol 11 (3) ◽  
pp. 287-298 ◽  
Author(s):  
Bernard La Scola
Keyword(s):  
Mass Spectrometry ◽  
Intact Cell ◽  
Bloodstream Infections ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

scholarly journals Erysipelothrix rhusiopathiae bacteremia without endocarditis: rapid identification from positive blood culture by MALDI-TOF mass spectrometry. A case report and literature review

2016 ◽  
Vol 8 (1) ◽  
Author(s):  
Luigi Principe ◽  
Silvia Bracco ◽  
Carola Mauri ◽  
Silvia Tonolo ◽  
Beatrice Pini ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Susceptibility Testing ◽  
Bloodstream Infections ◽  
Rapid Identification ◽  
Erysipelothrix Rhusiopathiae ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
High Incidence ◽  
Appropriate Therapy ◽  
Cutaneous Form

<em>Erysipelothrix</em> <em>rhusiopathiae</em> is a Gram-positive bacillus that is infrequently responsible for infections in humans. Three forms have been classified: a localized cutaneous form (erysipeloid) caused by traumatic penetration of <em>E.</em> <em>rhusiopathiae</em>, a generalized cutaneous form and a septicemic form. The latter type of disease has been previously associated with a high incidence of endocarditis. Here we report a case of <em>E. rhusiopathiae</em> bacteremia in a 74- year-old man, probably started from an erysipeloid form, in which endocarditis did not develop. This case presents some particular and uncommon features: i) no correlation with animal source; ii) correlation between bacteremia and erysipeloid lesion; iii) absence of endocarditis. MALDI-TOF mass spectrometry allowed to obtain a rapid identification (within 4 hours from bottle positivity) of <em>E. rhusiopathiae</em>. Together with direct antimicrobial susceptibility testing, this approach could improve the rate of appropriate therapy for bloodstream infections due to this fastidious pathogen.

scholarly journals Intact-Cell MALDI-ToF Mass Spectrometry for the Authentication of Drug-Adapted Cancer Cell Lines

Cells ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 1194 ◽  
Author(s):  
Jane F. Povey ◽  
Emily Saintas ◽  
Adewale V. Aderemi ◽  
Florian Rothweiler ◽  
Richard Zehner ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cell Line ◽  
Cell Lines ◽  
Mammalian Cells ◽  
Intact Cell ◽  
Mass Spectrometry Analysis ◽  
Genetic Origin ◽  
Whole Cell ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

The use of cell lines in research can be affected by cell line misidentification. Short tandem repeat (STR) analysis is an effective method, and the gold standard, for the identification of the genetic origin of a cell line, but methods that allow the discrimination between cell lines of the same genetic origin are lacking. Here, we use intact cell MALDI-ToF mass spectrometry analysis, routinely used for the identification of bacteria in clinical diagnostic procedures, for the authentication of a set of cell lines consisting of three parental neuroblastoma cell lines (IMR-5, IMR-32 and UKF-NB-3) and eleven drug-adapted sublines. Principal component analysis (PCA) of intact-cell MALDI-ToF mass spectrometry data revealed clear differences between most, but not all, of the investigated cell lines. Mass spectrometry whole-cell fingerprints enabled the separation of IMR-32 and its clonal subline IMR-5. Sublines that had been adapted to closely related drugs, for example, the cisplatin- and oxaliplatin-resistant UKF-NB-3 sublines and the vincristine- and vinblastine-adapted IMR-5 sublines, also displayed clearly distinctive patterns. In conclusion, intact whole-cell MALDI-ToF mass spectrometry has the potential to be further developed into an authentication method for mammalian cells of a common genetic origin.

Intact cell MALDI-TOF mass spectrometry, a promising proteomic profiling method in farm animal clinical and reproduction research

2020 ◽  
Vol 150 ◽  
pp. 113-121
Author(s):  
Laura Soler ◽  
Svetlana Uzbekova ◽  
Elisabeth Blesbois ◽  
Xavier Druart ◽  
Valérie Labas
Keyword(s):  
Mass Spectrometry ◽  
Intact Cell ◽  
Farm Animal ◽  
Proteomic Profiling ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

scholarly journals Rapid and reliable MALDI-TOF mass spectrometry identification of Candida non-albicans isolates from bloodstream infections

2013 ◽  
Vol 94 (3) ◽  
pp. 262-266 ◽  
Author(s):  
Giovanna Pulcrano ◽  
Dora Vita Iula ◽  
Antonio Vollaro ◽  
Alessandra Tucci ◽  
Monica Cerullo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Bloodstream Infections ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Mass Spectrometry Identification
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