Direct ethanol production from cellulosic materials at high temperature using the thermotolerant yeast Kluyveromyces marxianus displaying cellulolytic enzymes

2010 ◽  
Vol 88 (1) ◽  
pp. 381-388 ◽  
Author(s):  
Shuhei Yanase ◽  
Tomohisa Hasunuma ◽  
Ryosuke Yamada ◽  
Tsutomu Tanaka ◽  
Chiaki Ogino ◽  
...  
Keyword(s):  
High Temperature ◽  
Ethanol Production ◽  
Kluyveromyces Marxianus ◽  
Cellulolytic Enzymes ◽  
Thermotolerant Yeast ◽  
Cellulosic Materials

scholarly journals Kluyveromyces marxianus: Current State of Omics Studies, Strain Improvement Strategy and Potential Industrial Implementation

Fermentation ◽  
2020 ◽  
Vol 6 (4) ◽  
pp. 124
Author(s):  
Dung Minh Ha-Tran ◽  
Trinh Thi My Nguyen ◽  
Chieh-Chen Huang
Keyword(s):  
High Temperature ◽  
Ethanol Production ◽  
Kluyveromyces Marxianus ◽  
Plant Biomass ◽  
High Titer ◽  
Carbon Sources ◽  
Strain Improvement ◽  
Improvement Strategy ◽  
Yeast Saccharomyces Cerevisiae ◽  
Wide Range

Bioethanol is considered an excellent alternative to fossil fuels, since it importantly contributes to the reduced consumption of crude oil, and to the alleviation of environmental pollution. Up to now, the baker yeast Saccharomyces cerevisiae is the most common eukaryotic microorganism used in ethanol production. The inability of S. cerevisiae to grow on pentoses, however, hinders its effective growth on plant biomass hydrolysates, which contain large amounts of C5 and C12 sugars. The industrial-scale bioprocessing requires high temperature bioreactors, diverse carbon sources, and the high titer production of volatile compounds. These criteria indicate that the search for alternative microbes possessing useful traits that meet the required standards of bioethanol production is necessary. Compared to other yeasts, Kluyveromyces marxianus has several advantages over others, e.g., it could grow on a broad spectrum of substrates (C5, C6 and C12 sugars); tolerate high temperature, toxins, and a wide range of pH values; and produce volatile short-chain ester. K. marxianus also shows a high ethanol production rate at high temperature and is a Crabtree-negative species. These attributes make K. marxianus promising as an industrial host for the biosynthesis of biofuels and other valuable chemicals.

scholarly journals The potential of the newly isolated thermotolerant yeast Pichia kudriavzevii RZ8-1 for high-temperature ethanol production

2018 ◽  
Vol 49 (2) ◽  
pp. 378-391 ◽  
Author(s):  
Nuttaporn Chamnipa ◽  
Sudarat Thanonkeo ◽  
Preekamol Klanrit ◽  
Pornthap Thanonkeo
Keyword(s):  
High Temperature ◽  
Ethanol Production ◽  
Thermotolerant Yeast ◽  
Pichia Kudriavzevii

scholarly journals Physiological characterization of a new thermotolerant yeast strain isolated during Brazilian ethanol production and its application in high temperature fermentation

2020 ◽  
Author(s):  
Cleiton Dias do Prado ◽  
Gustavo Patricio Lorca Mandrujano ◽  
Jonas Paulino de Souza ◽  
Flavia Beatriz Sgobbi ◽  
Hosana Ribeiro Novaes ◽  
...  
Keyword(s):  
High Temperature ◽  
Ethanol Production ◽  
Second Generation ◽  
Thermotolerant Yeast ◽  
Secondary Products ◽  
High Concentration ◽  
Physiological Characterization ◽  
Thermotolerant Strain ◽  
First And Second Generation ◽  
Second Generation Ethanol

Abstract Background The use of thermotolerant yeast strains can improve the efficiency of ethanol fermentation, allowing fermentation to occur at temperatures higher than 40 °C. This increment in temperature could benefit traditional bio-ethanol production and allow simultaneous saccharification and fermentation (SSF) of starch or lignocellulosic biomass. Results We identified and characterized the physiology of a new thermotolerant strain able to fermentate at 40 °C while producing high yields of ethanol. Our results showed that, in comparison to the industrial yeast CAT-1, our strain was more resistant to various stressors generated during the production of first- and second-generation ethanol, and it also was able to change the pattern of genes involved in sucrose assimilation (SUC2 and AGT1). The formation of secondary products of fermentation was different at 40ºC, with reduced expression of genes involved in the formation of glycerol (GPD2), acetate (ALD6 and ALD4), and acetyl-CoA (ACS2). Conclusion The LBGA-01 strain is a thermotolerant strain that modulates the production of key genes, changing metabolic pathways during high-temperature fermentation, and increasing its tolerance to the high concentration of ethanol, sugar, acetic lactic, acetic acid, furfural and HMF. This indicates that this strain can be used to improve first- and second-generation ethanol production in Brazil.

scholarly journals High-Temperature Ethanol Fermentation and Transformation with Linear DNA in the Thermotolerant Yeast Kluyveromyces marxianus DMKU3-1042

2008 ◽  
Vol 74 (24) ◽  
pp. 7514-7521 ◽  
Author(s):  
Sanom Nonklang ◽  
Babiker M. A. Abdel-Banat ◽  
Kamonchai Cha-aim ◽  
Nareerat Moonjai ◽  
Hisashi Hoshida ◽  
...  
Keyword(s):  
High Temperature ◽  
Ethanol Production ◽  
Kluyveromyces Marxianus ◽  
Blot Hybridization ◽  
Molecular Genetic ◽  
Cost Effective ◽  
Southern Blot Hybridization ◽  
Heterologous Proteins ◽  
Linear Dna ◽  
Ethanol Producer

ABSTRACT We demonstrate herein the ability of Kluyveromyces marxianus to be an efficient ethanol producer and host for expressing heterologous proteins as an alternative to Saccharomyces cerevisiae. Growth and ethanol production by strains of K. marxianus and S. cerevisiae were compared under the same conditions. K. marxianus DMKU3-1042 was found to be the most suitable strain for high-temperature growth and ethanol production at 45°C. This strain, but not S. cerevisiae, utilized cellobiose, xylose, xylitol, arabinose, glycerol, and lactose. To develop a K. marxianus DMKU3-1042 derivative strain suitable for genetic engineering, a uracil auxotroph was isolated and transformed with a linear DNA of the S. cerevisiae ScURA3 gene. Surprisingly, Ura+ transformants were easily obtained. By Southern blot hybridization, the linear ScURA3 DNA was found to have inserted randomly into the K. marxianus genome. Sequencing of one Lys− transformant confirmed the disruption of the KmLYS1 gene by the ScURA3 insertion. A PCR-amplified linear DNA lacking K. marxianus sequences but containing an Aspergillus α-amylase gene under the control of the ScTDH3 promoter together with an ScURA3 marker was subsequently used to transform K. marxianus DMKU3-1042 in order to obtain transformants expressing Aspergillus α-amylase. Our results demonstrate that K. marxianus DMKU3-1042 can be an alternative cost-effective bioethanol producer and a host for transformation with linear DNA by use of S. cerevisiae-based molecular genetic tools.

High-Temperature Wine Making Using the Thermotolerant Yeast Strain Kluyveromyces marxianus IMB3

2004 ◽  
Vol 112 (1) ◽  
pp. 25-36 ◽  
Author(s):  
Y. Kourkoutas ◽  
C. Mcerlean ◽  
M. Kanellaki ◽  
C. J. Hack ◽  
R. Marchant ◽  
...  
Keyword(s):  
High Temperature ◽  
Kluyveromyces Marxianus ◽  
Yeast Strain ◽  
Thermotolerant Yeast ◽  
Wine Making
Sign in / Sign up

Export Citation Format

Share Document