scholarly journals Effect of culture operating conditions on succinate production in a multiphase fed-batch bioreactor using an engineered Escherichia coli strain

2011 ◽  
Vol 92 (3) ◽  
pp. 499-508 ◽  
Author(s):  
Jiangfeng Zhu ◽  
Chandresh Thakker ◽  
Ka-Yiu San ◽  
George Bennett
Keyword(s):  
Escherichia Coli ◽  
Coli Strain ◽  
Operating Conditions ◽  
Fed Batch ◽  
Succinate Production ◽  
Batch Bioreactor ◽  
Engineered Escherichia Coli

scholarly journals Tryptophan Production Maximization in a Fed-Batch Bioreactor with Modified E. coli Cells, by Optimizing Its Operating Policy Based on an Extended Structured Cell Kinetic Model

2021 ◽  
Vol 8 (12) ◽  
pp. 210
Author(s):  
Gheorghe Maria ◽  
Laura Renea
Keyword(s):  
Cell Model ◽  
Genetically Modify ◽  
Optimal Operation ◽  
Coli Strain ◽  
Operating Conditions ◽  
Model Complexity ◽  
State Variables ◽  
Fed Batch ◽  
E Coli ◽  
Batch Bioreactor

Hybrid kinetic models, linking structured cell metabolic processes to the dynamics of macroscopic variables of the bioreactor, are more and more used in engineering evaluations to derive more precise predictions of the process dynamics under variable operating conditions. Depending on the cell model complexity, such a math tool can be used to evaluate the metabolic fluxes in relation to the bioreactor operating conditions, thus suggesting ways to genetically modify the microorganism for certain purposes. Even if development of such an extended dynamic model requires more experimental and computational efforts, its use is advantageous. The approached probative example refers to a model simulating the dynamics of nanoscale variables from several pathways of the central carbon metabolism (CCM) of E. coli cells, linked to the macroscopic state variables of a fed-batch bioreactor (FBR) used for the tryptophan (TRP) production. The used E. coli strain was modified to replace the PTS system for glucose (GLC) uptake with a more efficient one. The study presents multiple elements of novelty: (i) the experimentally validated modular model itself, and (ii) its efficiency in computationally deriving an optimal operation policy of the FBR.

scholarly journals An Engineered Escherichia coli Strain with Synthetic Metabolism for in‐Cell Production of Translationally Active Methionine Derivatives

ChemBioChem ◽  
2020 ◽  
Vol 21 (24) ◽  
pp. 3525-3538
Author(s):  
Christian Johannes Schipp ◽  
Ying Ma ◽  
Ammar Al‐Shameri ◽  
Federico D'Alessio ◽  
Peter Neubauer ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Coli Strain ◽  
Cell Production ◽  
Engineered Escherichia Coli
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