Diversity analysis of the bacterial community in tobacco waste extract during reconstituted tobacco process

2014 ◽  
Vol 99 (1) ◽  
pp. 469-476 ◽  
Author(s):  
Huagui Liu ◽  
Houlong He ◽  
Changhe Cheng ◽  
Jinli Liu ◽  
Ming Shu ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Diversity Analysis ◽  
Waste Extract ◽  
Tobacco Waste

scholarly journals Diversity Analysis of Bacterial Community from Permafrost Soil of Mo-he in China

2013 ◽  
Vol 54 (1) ◽  
pp. 111-113 ◽  
Author(s):  
Dong Dan ◽  
Dian-peng Zhang ◽  
Wei-cheng Liu ◽  
Cai-ge Lu ◽  
Tao-tao Zhang
Keyword(s):  
Bacterial Community ◽  
Permafrost Soil ◽  
Diversity Analysis

scholarly journals EFEKTIVITAS LIMBAH TEMBAKAU TERHADAP WERENG COKLAT DAN PENGARUHNYA TERHADAP LABA-LABA PREDATOR

2017 ◽  
Vol 29 (1) ◽  
pp. 17
Author(s):  
Harlina Kusuma Tuti ◽  
Retno Wijayanti ◽  
Supriyadi Supriyadi
Keyword(s):  
Rice Field ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Design Experiment ◽  
Botanical Insecticide ◽  
Randomized Design ◽  
Plant Pest ◽  
Tobacco Roots ◽  
Waste Extract ◽  
Tobacco Waste

<p><em>Nilaparvata lugens Stal.is one of plant pest which damages rice field. The waste of tobacco contains nicotine which can use as botanical insecticide. This research was aimed to find out the effectiveness of tobacco waste in controlling brown planthopper and its effect then predator (Oxyopes javanus). The methode of this research applies Completed Randomized Design experiment. In this research, used the kind of tobacco waste and various concentration with triple repeating. The variables of this research</em><br /> <em>were mortality and death period of brown planthopper, mortality and eating capability of O. javanus viewed from direct and indirect application. The concentration 12,5% of tobacco roots extract cause mortality of brown planthopper reaches 93%. Furthermore, the concentration 12,5% of tobacco root extracts made the death period of brown planthopper faster than the other concentrations. Direct application of tobacco waste extracts did not cause of O. javanus. Indirect application of tobacco waste extract did not cause of death toward O. javanus, but caused eating capability of O .javanus. Extract from tobacco bark with concentration 3,125% dan 1,56% had the power repellent which was shown by O. javanus only eat 2,33 brown planthoppers per week.</em></p>

Metagenomics and Culture-Based Diversity Analysis of the Bacterial Community in the Zharkent Geothermal Spring in Kazakhstan

2021 ◽  
Author(s):  
Akzhigit Mashzhan ◽  
Rubén Javier-López ◽  
Aida Kistaubayeva ◽  
Irina Savitskaya ◽  
Nils-Kåre Birkeland
Keyword(s):  
Bacterial Community ◽  
Diversity Analysis ◽  
Geothermal Spring

scholarly journals Diversity and Co-Occurrence Patterns of microbial Communities in the Fertilized Eggs of Thitarodes Insect, the Implication on the Occurrence of Chinese cordyceps

2020 ◽  
Author(s):  
Lian-Xian Guo ◽  
Xiao-Shan Liu ◽  
Zhan-Hua Mai ◽  
Yue-Hui Hong ◽  
Qi-Jiong Zhu ◽  
...  
Keyword(s):  
Microbial Community ◽  
Network Analysis ◽  
Bacterial Community ◽  
Large Scale ◽  
Diversity Analysis ◽  
Its Sequencing ◽  
Β Diversity ◽  
Α Diversity ◽  
16S Rna ◽  
Fungal Structure

Abstract Background The large-scale artificial cultivation of Chinese cordyceps has not been widely implemented because the crucial factors triggering the occurrence of Chinese cordyceps have not been fully illuminated. Methods In this study, the bacterial and fungal structure of fertilized eggs in the host Thitarodes collected from 3 sampling sites with different occurrence rates of Chinese cordyceps (Sites A, B and C: high, low and null Chinese cordyceps, respectively) were analyzed by performing 16S RNA and ITS sequencing, respectively. And the intra-kingdom and inter-kingdom network were analyzed. Results For bacterial community, totally 4671 bacterial OTUs were obtained. α-diversity analysis revealed that the evenness of the eggs from site A was significantly higher than that of sites B and C, and the dominance index of site A was significantly lower than that of sites B and C ( P < 0.05). β-diversity analysis showed that the differences of bacterial community among the eggs from the three sampling sites were significantly different. OTU1 ( Wolbachia ) was the overwhelming predominant bacteria in the eggs from sites B and C. Although OTU4 ( Spiroplasma ) was detected in minor abundances, it showed distribution preference in the fertilized eggs from site A. For fungal community, totally 3318 fungal OTUs were obtained. Difference analysis showed significant differences among the three sites, while the differences were not as significant as that of bacterial community. In addition, ten fungal genera in the three most concerned Cordyceps families (Clavicipitaceae, Cordycipitaceae and Ophiocordycipitaceae) were detected in the fertilized eggs, while the most approved anamorph ( Hirsutella ) of Chinese cordyceps wasn’t discovered. Intra-kingdom (fungi) network analysis revealed more positive correlations and average degrees at sites A, and Inter-kingdom network analysis revealed more positive and negative correlations at sites A. Discussion The microbial community, especially the bacterial community in the fertilized eggs, might be significantly related with the occurrence of Chinese cordyceps, and Wolbachia might be the most significant microorganism negatively related with the occurrence. A closer correlation of the microbial community, especially closer fungal positive correlation, in the fertilized eggs might help for the occurrence of Chinese cordyceps.

In-depth diversity analysis of the bacterial community resident in the camel rumen

2015 ◽  
Vol 38 (1) ◽  
pp. 67-76 ◽  
Author(s):  
Javad Gharechahi ◽  
Hossein Shahbani Zahiri ◽  
Kambiz Akbari Noghabi ◽  
Ghasem Hosseini Salekdeh
Keyword(s):  
Bacterial Community ◽  
Diversity Analysis ◽  
Community Resident

scholarly journals Conjunctival Sac Microbiome in Infectious Conjunctivitis

2021 ◽  
Vol 9 (10) ◽  
pp. 2095
Author(s):  
Yasser Mohamed ◽  
Masafumi Uematsu ◽  
Yoshitomo Morinaga ◽  
Hien-Anh Thi Nguyen ◽  
Michiko Toizumi ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Ribosomal Rna ◽  
Diversity Analysis ◽  
Bacterial Conjunctivitis ◽  
Prevention Of Infection ◽  
Bacterial Phyla ◽  
Commensal Microbiota ◽  
Conventional Culture ◽  
Significant Difference ◽  
Unweighted Unifrac

Acute bacterial conjunctival infections are common, and this study identified the conjunctival bacterial community in infectious conjunctivitis cases seen at the outpatient clinic of Khanh Hoa General Hospital in Nha Trang, Vietnam from October 2016 through December 2017. Conjunctival swabs were collected and tested using conventional culture, PCR, and 16S ribosomal RNA sequencing. The study included 47 randomly selected patients. More than 98% of all DNA reads represented five bacterial phyla. Three of these phyla constituted 92% of all sequences (Firmicutes (35%), Actinobacteria (31%), and Proteobacteria (26%)). At the genus level, there were 12 common genera that constituted about 61% of all sequence reads. Seven of those genera were common (Streptococcus (10%), Cutibacterium (10%), Staphylococcus (7%), Nocardioides (7%), Corynebacterium 1 (5%), Anoxybacillus (5%), and Acinetobacter (5%)), which encompassed 49% of all reads. As for diversity analysis, there was no difference on PERMANOVA analysis (unweighted UniFrac) for sex (p = 0.087), chemosis (p = 0.064), and unclassified eyedrops (p = 0.431). There was a significant difference in cases with bilateral conjunctivitis (p = 0.017) and for using antibiotics (p = 0.020). Of the predominant phyla, Firmicutes had the highest abundance in bacterial conjunctivitis in this study. Pseudomonas as a resident commensal microbiota may have an important role in the prevention of infection.

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