Microwave and ultrasound pre-treatments influence microbial community structure and digester performance in anaerobic digestion of waste activated sludge

2016 ◽  
Vol 100 (12) ◽  
pp. 5339-5352 ◽  
Author(s):  
Maria Westerholm ◽  
Sam Crauwels ◽  
Maarten Van Geel ◽  
Raf Dewil ◽  
Bart Lievens ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Anaerobic Digestion ◽  
Activated Sludge ◽  
Microbial Community Structure ◽  
Waste Activated Sludge

Methane production and microbial community structure for alkaline pretreated waste activated sludge

2014 ◽  
Vol 169 ◽  
pp. 496-501 ◽  
Author(s):  
Rui Sun ◽  
Defeng Xing ◽  
Jianna Jia ◽  
Aijuan Zhou ◽  
Lu Zhang ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Activated Sludge ◽  
Microbial Community Structure ◽  
Methane Production ◽  
Waste Activated Sludge

scholarly journals Microbial Community Structure and Enumeration of Bacillus species in Activated Sludge

2017 ◽  
Vol 15 (6) ◽  
pp. 233-240 ◽  
Author(s):  
Masashi Hatamoto ◽  
Tomoyo Kaneko ◽  
Yuya Takimoto ◽  
Tsukasa Ito ◽  
Naoki Miyazato ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Activated Sludge ◽  
Microbial Community Structure

scholarly journals Thermophilic Alkaline Fermentation Followed by Mesophilic Anaerobic Digestion for Efficient Hydrogen and Methane Production from Waste-Activated Sludge: Dynamics of Bacterial Pathogens as Revealed by the Combination of Metagenomic and Quantitative PCR Analyses

2018 ◽  
Vol 84 (6) ◽  
Author(s):  
Jingjing Wan ◽  
Yuhang Jing ◽  
Yue Rao ◽  
Shicheng Zhang ◽  
Gang Luo
Keyword(s):  
Microbial Community ◽  
Anaerobic Digestion ◽  
Activated Sludge ◽  
Quantitative Pcr ◽  
Methane Production ◽  
Bacterial Pathogens ◽  
Methane Yield ◽  
Metagenomic Analysis ◽  
Waste Activated Sludge ◽  
Hydrogen Yield

ABSTRACT Thermophilic alkaline fermentation followed by mesophilic anaerobic digestion (TM) for hydrogen and methane production from waste-activated sludge (WAS) was investigated. The TM process was also compared to a process with mesophilic alkaline fermentation followed by a mesophilic anaerobic digestion (MM) and one-stage mesophilic anaerobic digestion (M) process. The results showed that both hydrogen yield (74.5 ml H 2 /g volatile solids [VS]) and methane yield (150.7 ml CH 4 /g VS) in the TM process were higher than those (6.7 ml H 2 /g VS and 127.8 ml CH 4 /g VS, respectively) in the MM process. The lowest methane yield (101.2 ml CH 4 /g VS) was obtained with the M process. Taxonomic results obtained from metagenomic analysis showed that different microbial community compositions were established in the hydrogen reactors of the TM and MM processes, which also significantly changed the microbial community compositions in the following methane reactors compared to that with the M process. The dynamics of bacterial pathogens were also evaluated. For the TM process, the reduced diversity and total abundance of bacterial pathogens in WAS were observed in the hydrogen reactor and were further reduced in the methane reactor, as revealed by metagenomic analysis. The results also showed not all bacterial pathogens were reduced in the reactors. For example, Collinsella aerofaciens was enriched in the hydrogen reactor, which was also confirmed by quantitative PCR (qPCR) analysis. The study further showed that qPCR was more sensitive for detecting bacterial pathogens than metagenomic analysis. Although there were some differences in the relative abundances of bacterial pathogens calculated by metagenomic and qPCR approaches, both approaches demonstrated that the TM process was more efficient for the removal of bacterial pathogens than the MM and M processes. IMPORTANCE This study developed an efficient process for bioenergy (H 2 and CH 4 ) production from WAS and elucidates the dynamics of bacterial pathogens in the process, which is important for the utilization and safe application of WAS. The study also made an attempt to combine metagenomic and qPCR analyses to reveal the dynamics of bacterial pathogens in anaerobic processes, which could overcome the limitations of each method and provide new insights regarding bacterial pathogens in environmental samples.

Effect of H2 addition on the microbial community structure of a mesophilic anaerobic digestion system

Energy ◽  
2020 ◽  
Vol 198 ◽  
pp. 117368 ◽  
Author(s):  
Xianpu Zhu ◽  
Liumeng Chen ◽  
Yichao Chen ◽  
Qin Cao ◽  
Xiaofeng Liu ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Anaerobic Digestion ◽  
Microbial Community Structure ◽  
H2 Addition
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