A combinatorial approach of N-terminus blocking and codon optimization strategies to enhance the soluble expression of recombinant hIL-7 in E. coli fed-batch culture

2016 ◽  
Vol 100 (23) ◽  
pp. 9979-9994 ◽  
Author(s):  
Nirmala Devi ◽  
Adivitiya ◽  
Yogender Pal Khasa
Keyword(s):  
Batch Culture ◽  
Codon Optimization ◽  
Soluble Expression ◽  
Combinatorial Approach ◽  
Fed Batch ◽  
E Coli ◽  
N Terminus ◽  
Fed Batch Culture

Production of K1 (Streptokinase) Using Batch and Fed-Batch Culture of Recombinant E. coli

1994 ◽  
pp. 447-453
Author(s):  
R. E. Narciandi ◽  
F. J. Morbe ◽  
U. Knupfer ◽  
R. Wenderoth ◽  
D. Riesenberge
Keyword(s):  
Batch Culture ◽  
Fed Batch ◽  
E Coli ◽  
Fed Batch Culture

scholarly journals Production of Poly(3-Hydroxybutyrate) by Fed-Batch Culture of Recombinant Escherichia coliwith a Highly Concentrated Whey Solution

2000 ◽  
Vol 66 (8) ◽  
pp. 3624-3627 ◽  
Author(s):  
Woo Suk Ahn ◽  
Si Jae Park ◽  
Sang Yup Lee
Keyword(s):  
Escherichia Coli ◽  
Batch Culture ◽  
Fed Batch ◽  
Batch Cultures ◽  
E Coli ◽  
Alcaligenes Latus ◽  
Stable Plasmid ◽  
Ph Stat ◽  
Fed Batch Culture

ABSTRACT Fermentation strategies for the production of poly(3-hydroxybutyrate) (PHB) from whey by recombinantEscherichia coli strain CGSC 4401 harboring theAlcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes were developed. The pH-stat fed-batch cultures of E. coli CGSC 4401 harboring pJC4, a stable plasmid containing theA. latus PHA biosynthesis genes, were carried out with a concentrated whey solution containing 280 g of lactose equivalent per liter. Final cell and PHB concentrations of 119.5 and 96.2 g/liter, respectively, were obtained in 37.5 h, which resulted in PHB productivity of 2.57 g/liter/h.

Production of Soluble Human Interleukin-6 in Cytoplasm by Fed-Batch Culture of Recombinant E. coli

2008 ◽  
Vol 21 (2) ◽  
pp. 524-531 ◽  
Author(s):  
Tae Wan Kim ◽  
Bong Hyun Chung ◽  
Yong Keun Chang
Keyword(s):  
Batch Culture ◽  
Interleukin 6 ◽  
Fed Batch ◽  
E Coli ◽  
Fed Batch Culture
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