Industrial control of recombinant E. coli fed-batch culture: new perspectives on traditional controlled variables

2002 ◽  
Vol 25 (2) ◽  
pp. 111-120 ◽  
Author(s):  
Johnston W. ◽  
R. Cord-Ruwisch ◽  
Cooney M.
Author(s):  
R. E. Narciandi ◽  
F. J. Morbe ◽  
U. Knupfer ◽  
R. Wenderoth ◽  
D. Riesenberge

2000 ◽  
Vol 66 (8) ◽  
pp. 3624-3627 ◽  
Author(s):  
Woo Suk Ahn ◽  
Si Jae Park ◽  
Sang Yup Lee

ABSTRACT Fermentation strategies for the production of poly(3-hydroxybutyrate) (PHB) from whey by recombinantEscherichia coli strain CGSC 4401 harboring theAlcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes were developed. The pH-stat fed-batch cultures of E. coli CGSC 4401 harboring pJC4, a stable plasmid containing theA. latus PHA biosynthesis genes, were carried out with a concentrated whey solution containing 280 g of lactose equivalent per liter. Final cell and PHB concentrations of 119.5 and 96.2 g/liter, respectively, were obtained in 37.5 h, which resulted in PHB productivity of 2.57 g/liter/h.


2008 ◽  
Vol 21 (2) ◽  
pp. 524-531 ◽  
Author(s):  
Tae Wan Kim ◽  
Bong Hyun Chung ◽  
Yong Keun Chang

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