Development of a versatile high-temperature short-time (HTST) pasteurization device for small-scale processing of cell culture medium formulations

2018 ◽  
Vol 102 (13) ◽  
pp. 5495-5504 ◽  
Author(s):  
Patrick Floris ◽  
Sean Curtin ◽  
Christian Kaisermayer ◽  
Anna Lindeberg ◽  
Jonathan Bones
Keyword(s):  
Cell Culture ◽  
High Temperature ◽  
Culture Medium ◽  
Small Scale ◽  
Cell Culture Medium ◽  
High Temperature Short Time ◽  
Short Time

Untargeted LC-MS/MS Profiling of Cell Culture Media Formulations for Evaluation of High Temperature Short Time Treatment Effects

2017 ◽  
Vol 89 (18) ◽  
pp. 9953-9960 ◽  
Author(s):  
Patrick Floris ◽  
Nicola McGillicuddy ◽  
Simone Albrecht ◽  
Brian Morrissey ◽  
Christian Kaisermayer ◽  
...  
Keyword(s):  
Cell Culture ◽  
High Temperature ◽  
Treatment Effects ◽  
Culture Media ◽  
Cell Culture Media ◽  
Time Treatment ◽  
High Temperature Short Time ◽  
Short Time

Implementing high-temperature short-time media treatment in commercial-scale cell culture manufacturing processes

2013 ◽  
Vol 98 (7) ◽  
pp. 2965-2971 ◽  
Author(s):  
Michael Pohlscheidt ◽  
Salim Charaniya ◽  
Fikret Kulenovic ◽  
Mahalia Corrales ◽  
Masaru Shiratori ◽  
...  
Keyword(s):  
Cell Culture ◽  
High Temperature ◽  
Manufacturing Processes ◽  
Commercial Scale ◽  
High Temperature Short Time ◽  
Short Time

Modeling Alkaline Phosphatase Inactivation in Bovine Milk During High-Temperature Short-Time Pasteurization

2001 ◽  
Vol 7 (6) ◽  
pp. 479-485 ◽  
Author(s):  
Q. Lu ◽  
P. Piyasena ◽  
G. S. Mittal
Keyword(s):  
Alkaline Phosphatase ◽  
High Temperature ◽  
Thermal Inactivation ◽  
Capillary Tube ◽  
Bovine Milk ◽  
Nonlinear Function ◽  
Small Scale ◽  
High Temperature Short Time ◽  
Different Temperatures ◽  
Short Time

Alkaline phosphatase (AP) is used as the indicator enzyme for proper pasteurization of bovine milk. Predictive modeling of AP inactivation during high-temperature short-time (HTST) pasteurization would support regulations; thus ensuring the safety of heat treated milk. Activation energy (Ea) of AP in milk was measured experimentally using the capillary tube method, and Ea was found to be 429252 J/mol. The Ea was used to develop a nonlinear model to describe the thermal inactivation of milk in a small-scale HTST pasteurizer with a plate heat exchanger. Integrated pasteurization effect (PE) was obtained at different holding temperatures (62–72°C) and holding times (3–25 s), by converting times at different temperatures in various sections of the pasteurizer to the equivalent time at the reference temperature (72°C). A nonlinear function was developed to relate the log(% residual AP activity) to PE. The r2 varied from 0.7488 to 0.8311. The validation trial indicated that the model could predict AP activity accurately for the% residual AP activity >1%.

3-iodothyronamine (T1AM) is taken up and rapidly metabolized in cell culture medium only at low concentration

2020 ◽  
Author(s):  
Federica Saponaro ◽  
Marco Borsò ◽  
Sara Verlotta ◽  
Lavinia Bandini ◽  
Alessandro Saba ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Low Concentration
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