Molecular breeding of the basidiomycete Coprinus cinereus strains with high lignin-decolorization and -degradation activities using novel heterologous protein expression vectors

1998 ◽  
Vol 49 (3) ◽  
pp. 285-289 ◽  
Author(s):  
K. Ogawa ◽  
T. Yamazaki ◽  
T. Hasebe ◽  
S. Kajiwara ◽  
A. Watanabe ◽  
...  
Keyword(s):  
Protein Expression ◽  
Molecular Breeding ◽  
Heterologous Protein ◽  
Coprinus Cinereus ◽  
Expression Vectors ◽  
Heterologous Protein Expression

scholarly journals Spontaneous excision of BAC vector sequences from bacmid-derived baculovirus expression vectors upon passage in insect cells

2003 ◽  
Vol 84 (10) ◽  
pp. 2669-2678 ◽  
Author(s):  
Gorben P. Pijlman ◽  
Jessica E. van Schijndel ◽  
Just M. Vlak
Keyword(s):  
Protein Expression ◽  
Viral Genome ◽  
Large Scale ◽  
Heterologous Protein ◽  
Insect Cells ◽  
Expression Cassette ◽  
Expression Vectors ◽  
Green Fluorescent Protein Gene ◽  
Heterologous Protein Expression ◽  
Scale Production

Repeated baculovirus infections in cultured insect cells lead to the generation of defective interfering viruses (DIs), which accumulate at the expense of the intact helper virus and compromise heterologous protein expression. In particular, Autographa californica multicapsid nucleopolyhedovirus (AcMNPV) DIs are enriched in an origin of viral DNA replication (ori) not associated with the homologous regions (hrs). This non-hr ori is located within the coding sequence of the non-essential p94 gene. We investigated the effect of a deletion of the AcMNPV non-hr ori on the heterologous protein expression levels following serial passage in Sf21 insect cells. Using homologous ET recombination in E. coli, deletions within the p94 gene were made in a bacterial artificial chromosome (BAC) containing the entire AcMNPV genome (bacmid). All bacmids were equipped with an expression cassette containing the green fluorescent protein gene and a gene encoding the classical swine fever virus E2 glycoprotein (CSFV-E2). For the parental (intact) bacmid only, a strong accumulation of DIs with reiterated non-hr oris was observed. This was not observed for the mutants, indicating that removal of the non-hr ori enhanced the genetic stability of the viral genome upon passaging. However, for all passaged viruses it was found that the entire BAC vector including the expression cassette was spontaneously deleted from the viral genome, leading to a rapid decrease in GFP and CSFV-E2 production. The rationale for the (intrinsic) genetic instability of the BAC vector in insect cells and the implications with respect to large-scale production of proteins with bacmid-derived baculoviruses are discussed.

Overexpression of the regulatory subunit of protein kinase A increases heterologous protein expression in Pichia pastoris

2020 ◽  
Vol 42 (12) ◽  
pp. 2685-2692 ◽  
Author(s):  
Xihao Liao ◽  
Wenyang Lin ◽  
Nanzhu Chen ◽  
Lu Li ◽  
Dafu Huang ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Pichia Pastoris ◽  
Protein Expression ◽  
Protein Kinase A ◽  
Heterologous Protein ◽  
Regulatory Subunit ◽  
Heterologous Protein Expression ◽  
Kinase A

Transport proteins PotD and Crr of Escherichia coli, novel fusion partners for heterologous protein expression

2007 ◽  
Vol 1774 (12) ◽  
pp. 1536-1543 ◽  
Author(s):  
Kyung-Yeon Han ◽  
Hyuk-Seong Seo ◽  
Jong-Am Song ◽  
Keum-Young Ahn ◽  
Jin-Seung Park ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Protein Expression ◽  
Heterologous Protein ◽  
Transport Proteins ◽  
Heterologous Protein Expression

scholarly journals Heterologous protein expression in E. coli v3 (protocols.io.9vgh63w)

protocols.io ◽  
2019 ◽  
Author(s):  
Diep R ◽  
Timothy Rhodes ◽  
Nay Chi ◽  
Estee E ◽  
Kai Xun
Keyword(s):  
Protein Expression ◽  
Heterologous Protein ◽  
Heterologous Protein Expression ◽  
E Coli

scholarly journals Heterologous protein expression in E. coli v5 (protocols.io.bdjti4nn)

protocols.io ◽  
2020 ◽  
Author(s):  
Diep Ganguly ◽  
Timothy Rhodes ◽  
Nay Chi ◽  
Estee E ◽  
Kai Xun
Keyword(s):  
Protein Expression ◽  
Heterologous Protein ◽  
Heterologous Protein Expression ◽  
E Coli

Heterologous Protein Expression in Psychrophilic Hosts

2008 ◽  
pp. 365-379 ◽  
Author(s):  
Ermenegilda Parrilli ◽  
Angela Duilio ◽  
Maria Luisa Tutino
Keyword(s):  
Protein Expression ◽  
Heterologous Protein ◽  
Heterologous Protein Expression
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