Strategies for optimizing heterologous protein expression in Escherichia coli

Synonymous codon changes at the 5′-end of the gene strongly impact the heterologous protein expression in Escherichia coli

Applied Biochemistry and Microbiology ◽

10.1134/s0003683817030024 ◽

2017 ◽

Vol 53 (3) ◽

pp. 296-303 ◽

Cited By ~ 2

Author(s):

S. Bashir ◽

M. Iqbal ◽

S. Sadaf ◽

M. W. Akhtar

Keyword(s):

Escherichia Coli ◽

Protein Expression ◽

Heterologous Protein ◽

Synonymous Codon ◽

Rare codon priority and its position specificity at the 5′ of the gene modulates heterologous protein expression in Escherichia coli

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2008.09.024 ◽

2008 ◽

Vol 376 (4) ◽

pp. 647-652 ◽

Cited By ~ 17

Author(s):

Chinnathambi Thangadurai ◽

Pichaimuthu Suthakaran ◽

Pankaj Barfal ◽

Balaiah Anandaraj ◽

Satya Narayan Pradhan ◽

...

Keyword(s):

Escherichia Coli ◽

Protein Expression ◽

Heterologous Protein ◽

Rare Codon ◽

Position Specificity

Two novel heat shock genes encoding proteins produced in response to heterologous protein expression in Escherichia coli.

Journal of Bacteriology ◽

10.1128/jb.174.21.6938-6947.1992 ◽

1992 ◽

Vol 174 (21) ◽

pp. 6938-6947 ◽

Cited By ~ 191

Author(s):

S P Allen ◽

J O Polazzi ◽

J K Gierse ◽

A M Easton

Keyword(s):

Escherichia Coli ◽

Heat Shock ◽

Protein Expression ◽

Heterologous Protein ◽

Heat Shock Genes ◽

Genes Encoding

A novel method for promoting heterologous protein expression in Escherichia coli by fusion with the HIV-1 TAT core domain

Amino Acids ◽

10.1007/s00726-010-0534-2 ◽

2010 ◽

Vol 39 (3) ◽

pp. 811-820 ◽

Cited By ~ 13

Author(s):

Yonghong Wu ◽

Changhong Ren ◽

Yan Gao ◽

Bing Hou ◽

Tingfang Chen ◽

...

Keyword(s):

Escherichia Coli ◽

Protein Expression ◽

Heterologous Protein ◽

Core Domain ◽

Novel Method ◽

Hiv 1

Strategies to maximize heterologous protein expression in Escherichia coli with minimal cost

Protein Expression and Purification ◽

10.1016/j.pep.2006.06.024 ◽

2007 ◽

Vol 51 (1) ◽

pp. 1-10 ◽

Cited By ~ 146

Author(s):

Wolfgang Peti ◽

Rebecca Page

Keyword(s):

Escherichia Coli ◽

Protein Expression ◽

Heterologous Protein ◽

Minimal Cost ◽

Corrigendum to Rare codon priority and its position specificity at the 5′ of the gene modulates heterologous protein expression in Escherichia coli

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2021.05.001 ◽

2021 ◽

Author(s):

Chinnathambi Thangadurai ◽

Pichaimuthu Suthakaran ◽

Pankaj Barfal ◽

Balaiah Anandaraj ◽

Satya Narayan Pradhan ◽

...

Keyword(s):

Escherichia Coli ◽

Protein Expression ◽

Heterologous Protein ◽

Rare Codon ◽

Position Specificity

A synergistic effect of suppressive CGG codon in +2 position and downstream CAT repeats for efficient heterologous protein expression in Escherichia coli

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2008.04.079 ◽

2008 ◽

Vol 371 (3) ◽

pp. 380-384 ◽

Cited By ~ 2

Author(s):

Vaishnavo Rabindranath Pai ◽

Vadivel Murugan

Keyword(s):

Escherichia Coli ◽

Synergistic Effect ◽

Protein Expression ◽

Heterologous Protein ◽

A screening strategy for heterologous protein expression in Escherichia coli with the highest return of investment

Protein Expression and Purification ◽

10.1016/j.pep.2011.08.030 ◽

2012 ◽

Vol 81 (1) ◽

pp. 33-41 ◽

Cited By ~ 21

Author(s):

Benny Pacheco ◽

Lissete Crombet ◽

Peter Loppnau ◽

Doug Cossar

Keyword(s):

Escherichia Coli ◽

Protein Expression ◽

Heterologous Protein ◽

Screening Strategy ◽

Return Of Investment

Enhancement of heterologous protein expression in Escherichia coli by co-expression of nonspecific DNA-binding stress protein, Dps

Enzyme and Microbial Technology ◽

10.1016/s0141-0229(03)00148-0 ◽

2003 ◽

Vol 33 (4) ◽

pp. 460-465 ◽

Cited By ~ 15

Author(s):

Young Soo Kim ◽

Jeong Hyun Seo ◽

Hyung Joon Cha

Keyword(s):

Escherichia Coli ◽

Dna Binding ◽

Protein Expression ◽

Heterologous Protein ◽

Stress Protein ◽

Transport proteins PotD and Crr of Escherichia coli, novel fusion partners for heterologous protein expression

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics ◽

10.1016/j.bbapap.2007.09.012 ◽

2007 ◽

Vol 1774 (12) ◽

pp. 1536-1543 ◽

Cited By ~ 15

Author(s):

Kyung-Yeon Han ◽

Hyuk-Seong Seo ◽

Jong-Am Song ◽

Keum-Young Ahn ◽

Jin-Seung Park ◽

...

Keyword(s):

Escherichia Coli ◽

Protein Expression ◽

Heterologous Protein ◽

Transport Proteins ◽

Heterologous Protein Expression