High-level expression of a recombinant protein in Klebsiella planticola owing to induced secretion into the culture medium

1999 ◽  
Vol 51 (5) ◽  
pp. 627-632 ◽  
Author(s):  
G. Miksch ◽  
R. Neitzel ◽  
K. Friehs ◽  
E. Flaschel
Keyword(s):  
Recombinant Protein ◽  
Culture Medium ◽  
High Level Expression ◽  
Klebsiella Planticola ◽  
High Level

A high-level expression vector containing selectable marker for continuous production of recombinant protein in insect cells

2009 ◽  
Vol 31 (5) ◽  
pp. 623-627 ◽  
Author(s):  
Takuya Shishido ◽  
Naoya Kurata ◽  
Myung Eui Yoon ◽  
Tsutomu Tanaka ◽  
Hideki Yamaji ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Expression Vector ◽  
Insect Cells ◽  
Selectable Marker ◽  
Continuous Production ◽  
High Level Expression ◽  
High Level

scholarly journals High-level secretion of a recombinant protein to the culture medium with aBacillus subtilistwin-arginine translocation system inEscherichia coli

FEBS Journal ◽  
2013 ◽  
Vol 280 (16) ◽  
pp. 3810-3821 ◽  
Author(s):  
Anna M. Albiniak ◽  
Cristina F. R. O. Matos ◽  
Steven D. Branston ◽  
Robert B. Freedman ◽  
Eli Keshavarz-Moore ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Culture Medium ◽  
Inescherichia Coli ◽  
High Level

Thermostable multifunctional GH74 xyloglucanase from Myceliophthora thermophila: high-level expression in Pichia pastoris and characterization of the recombinant protein

2017 ◽  
Vol 101 (14) ◽  
pp. 5653-5666 ◽  
Author(s):  
Oksana V. Berezina ◽  
Jonathan Herlet ◽  
Sergey V. Rykov ◽  
Petra Kornberger ◽  
Artem Zavyalov ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Recombinant Protein ◽  
Myceliophthora Thermophila ◽  
High Level Expression ◽  
High Level

scholarly journals YscO of Yersinia pestis Is a Mobile Core Component of the Yop Secretion System

1998 ◽  
Vol 180 (15) ◽  
pp. 3882-3890 ◽  
Author(s):  
Patricia L. Payne ◽  
Susan C. Straley
Keyword(s):  
Yersinia Pestis ◽  
Gene Product ◽  
Culture Medium ◽  
Immunoblot Analysis ◽  
High Level Expression ◽  
Its Gene ◽  
Regulated Expression ◽  
V Antigen ◽  
High Level ◽  
Mobile Protein

ABSTRACT The Yersinia pestis low-Ca2+ response stimulon is responsible for the temperature- and Ca2+-regulated expression and secretion of plasmid pCD1-encoded antihost proteins (V antigen and Yops). We have previously shown that lcrD, yscC, yscD,yscG, and yscR encode proteins that are essential for high-level expression and secretion of V antigen and Yops at 37°C in the absence of Ca2+. In this study, we characterized yscO of the Yop secretion (ysc) operon that contains yscN through yscU by determining the localization of its gene product and the phenotype of an in-frame deletion. The yscO mutant grew and expressed the same levels of Yops as the parent at 37°C in the presence of Ca2+. In the absence of Ca2+, the mutant grew independently of Ca2+, expressed only basal levels of V antigen and Yops, and failed to secrete these. These defects could be partially complemented by providing yscO intrans in the yscO mutant. Overexpression of YopM and V antigen in the mutant failed to restore the export of either protein, showing that the mutation had a direct effect on secretion. These results indicated that the yscO gene product is required for high-level expression and secretion of V antigen and Yops. YscO was found by immunoblot analysis in the soluble and membrane fractions of bacteria growing at 37°C irrespective of the presence of Ca2+ and in the culture medium in the absence of Ca2+. YscO is the only mobile protein identified so far in the Yersinia species that is required for secretion of V antigen and Yops.

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