2-μm vectors containing theSaccharomyces cerevisiae metallothionein gene as a selectable marker: Excellent stability in complex media, and high-level expression of a recombinant protein from aCUP1-promoter-controlled expression cassette incis

Yeast ◽  
1995 ◽  
Vol 11 (1) ◽  
pp. 1-14 ◽  
Author(s):  
Thomas Hottiger ◽  
Jochen Kuhla ◽  
Gabriele Pohlig ◽  
Peter Fürst ◽  
Albert Spielmann ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Selectable Marker ◽  
Expression Cassette ◽  
Complex Media ◽  
High Level Expression ◽  
Metallothionein Gene ◽  
High Level ◽  
Excellent Stability

A high-level expression vector containing selectable marker for continuous production of recombinant protein in insect cells

2009 ◽  
Vol 31 (5) ◽  
pp. 623-627 ◽  
Author(s):  
Takuya Shishido ◽  
Naoya Kurata ◽  
Myung Eui Yoon ◽  
Tsutomu Tanaka ◽  
Hideki Yamaji ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Expression Vector ◽  
Insect Cells ◽  
Selectable Marker ◽  
Continuous Production ◽  
High Level Expression ◽  
High Level

Thermostable multifunctional GH74 xyloglucanase from Myceliophthora thermophila: high-level expression in Pichia pastoris and characterization of the recombinant protein

2017 ◽  
Vol 101 (14) ◽  
pp. 5653-5666 ◽  
Author(s):  
Oksana V. Berezina ◽  
Jonathan Herlet ◽  
Sergey V. Rykov ◽  
Petra Kornberger ◽  
Artem Zavyalov ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Recombinant Protein ◽  
Myceliophthora Thermophila ◽  
High Level Expression ◽  
High Level

scholarly journals Inducible Overexpression of a Toxic Protein by an Adenovirus Vector with a Tetracycline-Regulatable Expression Cassette

1998 ◽  
Vol 72 (3) ◽  
pp. 2289-2296 ◽  
Author(s):  
Bernard Massie ◽  
France Couture ◽  
Linda Lamoureux ◽  
Dick D. Mosser ◽  
Claire Guilbault ◽  
...  
Keyword(s):  
Cell Lines ◽  
Recombinant Proteins ◽  
Recombinant Adenovirus ◽  
Adenovirus Vector ◽  
Expression Cassette ◽  
Cell Protein ◽  
Animal Cells ◽  
High Level Expression ◽  
Simplex Virus ◽  
High Level

ABSTRACT We have constructed two new adenovirus expression cassettes that expand both the range of genes which can be expressed with adenovirus vectors (AdV) and the range of cells in which high-level expression can be attained. By inclusion of a tetracycline-regulated promoter in the transfer vector pAdTR5, it is now possible to generate recombinant adenoviruses expressing proteins that are either cytotoxic or that interfere with adenovirus replication. We have used this strategy to generate a recombinant adenovirus encoding a deletion in the R1 subunit [R1(Δ2-357)] of the herpes simplex virus type 2 ribonucleotide reductase. Cell lines expressing the tetracycline-regulated transactivator (tTA) from an integrated vector or following infection with an AdV expressing tTA are able to produce ΔR1 protein at a level approaching 10% total cell protein (TCP) when infected with Ad5TR5ΔR1 before they subsequently die. To our knowledge, this is the first report of the overexpression of a toxic gene product with AdV. We have also constructed a new constitutive adenovirus expression cassette based on an optimized cytomegalovirus immediate-early promoter-enhancer that allows the expression of recombinant proteins at a level greater than 20% TCP in nonpermissive cell lines. Together, these new expression cassettes significantly improve the utility of the adenovirus system for high-level expression of recombinant proteins in animal cells and will undoubtedly find useful applications in gene therapy.

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