Modeling of solute transport and biological sulfate reduction using low cost electron donor

2008 ◽  
Vol 56 (8) ◽  
pp. 1605-1613 ◽  
Author(s):  
Osama Eljamal ◽  
Kenji Jinno ◽  
Tosao Hosokawa
Keyword(s):  
Solute Transport ◽  
Sulfate Reduction ◽  
Electron Donor ◽  
Low Cost ◽  
Biological Sulfate Reduction

scholarly journals Citric acid wastewater as electron donor for biological sulfate reduction

2009 ◽  
Vol 83 (5) ◽  
pp. 957-963 ◽  
Author(s):  
Alfons J. M. Stams ◽  
Jacco Huisman ◽  
Pedro A. Garcia Encina ◽  
Gerard Muyzer
Keyword(s):  
Citric Acid ◽  
Sulfate Reduction ◽  
Electron Donor ◽  
Biological Sulfate Reduction

scholarly journals Biological sulfate removal with low-cost carbon sources using cold-acclimated bacteria

2021 ◽  
Author(s):  
Hanna Virpiranta ◽  
Sanna Taskila ◽  
Tiina Leiviskä ◽  
Jouko Vepsäläinen ◽  
Jaakko Rämö ◽  
...  
Keyword(s):  
Sewage Sludge ◽  
Carbon Source ◽  
Sulfate Reduction ◽  
Low Cost ◽  
Carbon Sources ◽  
Reduction Process ◽  
Sulfate Removal ◽  
Cold Tolerant ◽  
Sulfate Reduction Process ◽  
Biological Sulfate Reduction

Abstract The main goal of this study was to develop a cost-efficient biological method for the removal of sulfate from mining effluents in cold conditions. A consortium of cold-tolerant sulfate-reducing bacteria (SRB) was tested at 6 °C regarding the utilization of economically viable, low-cost carbon sources, i.e., whey, conditioned sewage sludge, and peat, in the removal of sulfate from synthetic mining water. Succinate was used as a reference carbon source. Of all the studied low-cost carbon sources, conditioned sewage sludge proved to be the most efficient. Nuclear magnetic resonance (NMR) spectroscopy revealed that sewage sludge contained propionic acid, which proved to be utilizable by SRB under cold conditions. Peat both adsorbed the sulfate and acted as a nutrient source in the sulfate reduction process. When whey was used as a carbon source, only a slight decrease in sulfate concentration was detected. Succinate was found to work in a truly predictable and efficient way as a carbon source in biological sulfate reduction, even at the lowest concentration tested. The use of conditioned sewage sludge increased the bacterial diversity in liquid cultivations significantly. However, the number of SRB was highest in the succinate cultivations.

scholarly journals A Microcosm Treatability Study for Evaluating Wood Mulch-Based Amendments as Electron Donors for Trichloroethene (TCE) Reductive Dechlorination

Water ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 1949
Author(s):  
Edoardo Masut ◽  
Alessandro Battaglia ◽  
Luca Ferioli ◽  
Anna Legnani ◽  
Carolina Cruz Viggi ◽  
...  
Keyword(s):  
Electron Donor ◽  
Environmental Sustainability ◽  
Reductive Dechlorination ◽  
Environmental Remediation ◽  
Low Cost ◽  
Chlorinated Solvents ◽  
Electron Donors ◽  
Chlorinated Ethenes ◽  
Dehalococcoides Mccartyi ◽  
Wood Mulch

In this study, wood mulch-based amendments were tested in a bench-scale microcosm experiment in order to assess the treatability of saturated soils and groundwater from an industrial site contaminated by chlorinated ethenes. Wood mulch was tested alone as the only electron donor in order to assess its potential for stimulating the biological reductive dechlorination. It was also tested in combination with millimetric iron filings in order to assess the ability of the additive to accelerate/improve the bioremediation process. The efficacy of the selected amendments was compared with that of unamended control microcosms. The results demonstrated that wood mulch is an effective natural and low-cost electron donor to stimulate the complete reductive dechlorination of chlorinated solvents to ethene. Being a side-product of the wood industry, mulch can be used in environmental remediation, an approach which perfectly fits the principles of circular economy and addresses the compelling needs of a sustainable and low environmental impact remediation. The efficacy of mulch was further improved by the co-presence of iron filings, which accelerated the conversion of vinyl chloride into the ethene by increasing the H2 availability rather than by catalyzing the direct abiotic dechlorination of contaminants. Chemical analyses were corroborated by biomolecular assays, which confirmed the stimulatory effect of the selected amendments on the abundance of Dehalococcoides mccartyi and related reductive dehalogenase genes. Overall, this paper further highlights the application potential and environmental sustainability of wood mulch-based amendments as low-cost electron donors for the biological treatment of chlorinated ethenes.

scholarly journals Production of bio-xylitol from d-xylose by an engineered Pichia pastoris expressing a recombinant xylose reductase did not require any auxiliary substrate as electron donor

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Tai Man Louie ◽  
Kailin Louie ◽  
Samuel DenHartog ◽  
Sridhar Gopishetty ◽  
Mani Subramanian ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Electron Donor ◽  
Low Cost ◽  
Standard Procedure ◽  
Xylose Reductase ◽  
Animal Nutrition ◽  
Whole Cells ◽  
Reductase Gene ◽  
Biocatalytic Reaction ◽  
Ketone Reductases

Abstract Background Xylitol is a five-carbon sugar alcohol that has numerous beneficial health properties. It has almost the same sweetness as sucrose but has lower energy value compared to the sucrose. Metabolism of xylitol is insulin independent and thus it is an ideal sweetener for diabetics. It is widely used in food products, oral and personal care, and animal nutrition as well. Here we present a two-stage strategy to produce bio-xylitol from d-xylose using a recombinant Pichia pastoris expressing a heterologous xylose reductase gene. The recombinant P. pastoris cells were first generated by a low-cost, standard procedure. The cells were then used as a catalyst to make the bio-xylitol from d-xylose. Results Pichia pastoris expressing XYL1 from P. stipitis and gdh from B. subtilis demonstrated that the biotransformation was very efficient with as high as 80% (w/w) conversion within two hours. The whole cells could be re-used for multiple rounds of catalysis without loss of activity. Also, the cells could directly transform d-xylose in a non-detoxified hemicelluloses hydrolysate to xylitol at 70% (w/w) yield. Conclusions We demonstrated here that the recombinant P. pastoris expressing xylose reductase could transform d-xylose, either in pure form or in crude hemicelluloses hydrolysate, to bio-xylitol very efficiently. This biocatalytic reaction happened without the external addition of any NAD(P)H, NAD(P)+, and auxiliary substrate as an electron donor. Our experimental design & findings reported here are not limited to the conversion of d-xylose to xylitol only but can be used with other many oxidoreductase reactions also, such as ketone reductases/alcohol dehydrogenases and amino acid dehydrogenases, which are widely used for the synthesis of high-value chemicals and pharmaceutical intermediates.

Integrated acid leaching and biological sulfate reduction of phosphogypsum for REE recovery

2020 ◽  
Vol 155 ◽  
pp. 106408 ◽  
Author(s):  
Marja Salo ◽  
Oleg Knauf ◽  
Jarno Mäkinen ◽  
Xiaosheng Yang ◽  
Pertti Koukkari
Keyword(s):  
Sulfate Reduction ◽  
Acid Leaching ◽  
Biological Sulfate Reduction

Modeling solute transport and sulfate reduction in marsh sediments

1987 ◽  
Vol 51 (5) ◽  
pp. 1109-1120 ◽  
Author(s):  
William H. Casey ◽  
Antonio C. Lasaga
Keyword(s):  
Solute Transport ◽  
Sulfate Reduction ◽  
Marsh Sediments

Precipitation of heavy metals from coal ash leachate using biogenic hydrogen sulfide generated from FGD gypsum

2013 ◽  
Vol 67 (2) ◽  
pp. 311-318 ◽  
Author(s):  
Madawala Liyanage Duminda Jayaranjan ◽  
Ajit P. Annachhatre
Keyword(s):  
Heavy Metals ◽  
Hydrogen Sulfide ◽  
Sulfate Reduction ◽  
Bottom Ash ◽  
Coal Ash ◽  
Reduction Process ◽  
Fgd Gypsum ◽  
Sulfate Reducing ◽  
Sulfate Reduction Process ◽  
Biological Sulfate Reduction

Investigations were undertaken to utilize flue gas desulfurization (FGD) gypsum for the treatment of leachate from the coal ash (CA) dump sites. Bench-scale investigations consisted of three main steps namely hydrogen sulfide (H2S) production by sulfate reducing bacteria (SRB) using sulfate from solubilized FGD gypsum as the electron acceptor, followed by leaching of heavy metals (HMs) from coal bottom ash (CBA) and subsequent precipitation of HMs using biologically produced sulfide. Leaching tests of CBA carried out at acidic pH revealed the existence of several HMs such as Cd, Cr, Hg, Pb, Mn, Cu, Ni and Zn. Molasses was used as the electron donor for the biological sulfate reduction (BSR) process which produced sulfide rich effluent with concentration up to 150 mg/L. Sulfide rich effluent from the sulfate reduction process was used to precipitate HMs as metal sulfides from CBA leachate. HM removal in the range from 40 to 100% was obtained through sulfide precipitation.

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