Fetal rat glandular stomach epithelial cells differentiate into surface mucous cells which express cathepsin E in the absence of mesenchymal cells in primary culture

1994 ◽  
Vol 56 (1) ◽  
pp. 0083 ◽  
Author(s):  
Hiroshi Fukamachi ◽  
Masao Ichinose ◽  
Satoshi Ishihama ◽  
Shinko Tsukada ◽  
Sadao Yasugi ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Mesenchymal Cells ◽  
Glandular Stomach ◽  
Mucous Cells ◽  
Cathepsin E ◽  
Fetal Rat

Fetal rat glandular stomach epithelial cells differentiate into surface mucous cells which express cathepsin E in the absence of mesenchymal cells in primary culture

1994 ◽  
Vol 56 (1-2) ◽  
pp. 83-89 ◽  
Author(s):  
Hiroshi Fukamachi ◽  
Masao Ichinose ◽  
Satoshi Ishihama ◽  
Shinko Tsukada ◽  
Sadao Yasugi ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Mesenchymal Cells ◽  
Glandular Stomach ◽  
Mucous Cells ◽  
Cathepsin E ◽  
Fetal Rat

Cathepsin E is Expressed in Fetal Rat Glandular Stomach Epithelial Cells in Primary Culture in the Absence of Mesenchymes

1995 ◽  
pp. 357-361
Author(s):  
Hiroshi Fukamachi ◽  
Masao Ichinose ◽  
Satoshi Ishihama ◽  
Shinko Tsukada ◽  
Chie Furihata ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Glandular Stomach ◽  
Cathepsin E ◽  
Fetal Rat

Immunolocalisation of androgen receptor to interstitial cells in fetal rat testes and to mesenchymal and epithelial cells of associated ducts

1995 ◽  
Vol 147 (2) ◽  
pp. 285-293 ◽  
Author(s):  
G Majdic ◽  
M R Millar ◽  
P T K Saunders
Keyword(s):  
Androgen Receptor ◽  
Epithelial Cells ◽  
Leydig Cells ◽  
Interstitial Cells ◽  
Mesenchymal Cells ◽  
Wolffian Duct ◽  
Nuclear Staining ◽  
Mullerian Duct ◽  
Fetal Rat ◽  
Fetal Leydig Cells

Abstract Androgens are required for the development of male internal and external genitalia. Androgen action is mediated by an intracellular receptor which acts as a transcription factor following activation by ligand binding. The aim of the present study was to define the time of appearance of androgen receptor (AR) in the male fetal rat gonad using immunohistochemistry. Intact fetuses (days 13·5–16·5) or testicular tissue (days 16·5–20·5 and days 3–7 postnatal) were fixed in Bouins' solution and processed into paraffin wax. On day 16·5 nuclear AR were present in mesenchymal cells surrounding the Wolffian duct but those around the Mullerian duct were receptor negative. During the following day (17–18) the abundance of nuclear staining increased, becoming detectable in the epithelial cells of the Wolffian ducts. Within the testis some nuclear staining was apparent at day 17 but was confined to interstitial cells surrounding the seminiferous cords. As development of the testis proceeded the abundance of nuclear AR in peritubular and elongated mesenchymal cells increased. AR were not detected in fetal Leydig cells expressing 3β-hydroxysteroid dehydrogenase nor in the ovaries or associated ducts of female fetuses at the same ages. In conclusion, in the rat we have found AR expression detectable by immunohistochemistry in mesonephric mesenchyme to be confined to that underlying the Wolffian ducts and to be absent from the area around the degenerating Mullerian duct. On and after day 17 of gestation AR is present in Wolffian duct epithelial cell nuclei and within the testis it is confined to peritubular and interstitial cells which may have migrated from the mesonephros. Fetal Leydig cells were receptor negative. Within the seminiferous cords AR in Sertoli cells remained low until after birth and some perinuclear staining was detected in cells thought to be gonocytes. We believe this to be the first report of immunolocalisation of AR to fetal testicular interstitial cells. Journal of Endocrinology (1995) 147, 285–293

scholarly journals Expression of Cathepsin E and Its Suppression with Intestinalization in Epithelial Cells and Tumor Cells in Rat Glandular Stomach Treated with N-methyl-N'-nitro-N-nitrosoguanidine.

2001 ◽  
Vol 14 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Hirofumi Yuasa ◽  
Satoshi Yoneyama ◽  
Masao Ichinose ◽  
Kazumasa Miki ◽  
Tetsuya Tsukamoto ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Tumor Cells ◽  
Glandular Stomach ◽  
Cathepsin E

Establishment of a long-term three-dimensional primary culture of mouse glandular stomach epithelial cells within the stem cell niche

2013 ◽  
Vol 432 (4) ◽  
pp. 558-563 ◽  
Author(s):  
Takahito Katano ◽  
Akifumi Ootani ◽  
Tsutomu Mizoshita ◽  
Satoshi Tanida ◽  
Hironobu Tsukamoto ◽  
...  
Keyword(s):  
Stem Cell ◽  
Epithelial Cells ◽  
Primary Culture ◽  
Stem Cell Niche ◽  
Three Dimensional ◽  
Glandular Stomach ◽  
Cell Niche

Proliferation and differentiation of fetal rat intestinal epithelial cells in primary serum-free culture

1992 ◽  
Vol 103 (2) ◽  
pp. 511-519 ◽  
Author(s):  
H. Fukamachi
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Luminal Surface ◽  
Epithelial Proliferation ◽  
Serum Free ◽  
Fetal Rat ◽  
Proliferation And Differentiation ◽  
Fibroblastic Cells

It has been a subject of controversy whether fibroblastic cells are necessary for the proliferation of intestinal epithelial cells in primary culture. To answer this question, we have developed a serum-free primary culture system which allows reproducible and quantitative assays of proliferation and differentiation of fetal rat intestinal epithelial cells in the absence of fibroblastic cells. Pure intestinal epithelial tissues were obtained from 16.5-day fetal rats without contamination of mesenchymal cells, and were successfully cultured on a collagen gel in a medium consisting of Ham's F12, bovine serum albumin, epidermal growth factor (EGF), insulin, cholera toxin, transferrin and hydrocortisone. The epithelial nature of the cultured cells was confirmed by the presence of cytokeratin in the cells. Under optimal culture conditions, intestinal epithelial cells readily attached to the substratum in a day, and proliferated rapidly in vitro, increasing their number about 10 times in the first 5 days. EGF, insulin, cholera toxin, transferrin and hydrocortisone synergistically induced the epithelial proliferation, and lack of any one of them resulted in a significant reduction of the proliferation. In contrast, fetal bovine or horse serums, which have been widely used to supplement culture media, severely inhibited the epithelial proliferation. Histological examination showed that the epithelial cells formed simple cuboidal epithelia with basally-located nuclei when cultured on collagen gels. The intestinal epithelial nature of the cells was affirmed by the presence of villin on their luminal surface. Ultrastructurally, cells were connected by tight junctions and desmosomes at the subluminal region, and microvilli were projecting on the luminal surface, indicating that the cells in primary culture retained some characteristics of absorptive epithelial cells.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Growth of fetal rat gastro-intestinal epithelial cells is region-specifically controlled by growth factors and substrata in primary culture

1995 ◽  
Vol 37 (1) ◽  
pp. 11-19 ◽  
Author(s):  
Hiroshi Fukamachi ◽  
Masao Ichinose ◽  
Shinko Tsukada ◽  
Kiyoshi Kurokawa ◽  
Koichiro Shiokawa ◽  
...  
Keyword(s):  
Growth Factors ◽  
Epithelial Cells ◽  
Primary Culture ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Fetal Rat

Primary culture of canine prostate epithelial cells

1982 ◽  
Vol 7 (2) ◽  
pp. 51-54 ◽  
Author(s):  
Louis Terracio ◽  
William H. J. Douglas
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Prostate Epithelial Cells

Putrescine stimulates growth of human bronchial epithelial cells in primary culture

In Vitro ◽  
1980 ◽  
Vol 16 (5) ◽  
pp. 399-406 ◽  
Author(s):  
Gary D. Stoner ◽  
Curtis C. Harris ◽  
Gwendolyn A. Myers ◽  
Benjamin F. Trump ◽  
Robert D. Connor
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Bronchial Epithelial Cells ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial
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