scholarly journals Growth of fetal rat gastro-intestinal epithelial cells is region-specifically controlled by growth factors and substrata in primary culture

1995 ◽  
Vol 37 (1) ◽  
pp. 11-19 ◽  
Author(s):  
Hiroshi Fukamachi ◽  
Masao Ichinose ◽  
Shinko Tsukada ◽  
Kiyoshi Kurokawa ◽  
Koichiro Shiokawa ◽  
...  
Keyword(s):  
Growth Factors ◽  
Epithelial Cells ◽  
Primary Culture ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Fetal Rat

Proliferation and differentiation of fetal rat intestinal epithelial cells in primary serum-free culture

1992 ◽  
Vol 103 (2) ◽  
pp. 511-519 ◽  
Author(s):  
H. Fukamachi
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Luminal Surface ◽  
Epithelial Proliferation ◽  
Serum Free ◽  
Fetal Rat ◽  
Proliferation And Differentiation ◽  
Fibroblastic Cells

It has been a subject of controversy whether fibroblastic cells are necessary for the proliferation of intestinal epithelial cells in primary culture. To answer this question, we have developed a serum-free primary culture system which allows reproducible and quantitative assays of proliferation and differentiation of fetal rat intestinal epithelial cells in the absence of fibroblastic cells. Pure intestinal epithelial tissues were obtained from 16.5-day fetal rats without contamination of mesenchymal cells, and were successfully cultured on a collagen gel in a medium consisting of Ham's F12, bovine serum albumin, epidermal growth factor (EGF), insulin, cholera toxin, transferrin and hydrocortisone. The epithelial nature of the cultured cells was confirmed by the presence of cytokeratin in the cells. Under optimal culture conditions, intestinal epithelial cells readily attached to the substratum in a day, and proliferated rapidly in vitro, increasing their number about 10 times in the first 5 days. EGF, insulin, cholera toxin, transferrin and hydrocortisone synergistically induced the epithelial proliferation, and lack of any one of them resulted in a significant reduction of the proliferation. In contrast, fetal bovine or horse serums, which have been widely used to supplement culture media, severely inhibited the epithelial proliferation. Histological examination showed that the epithelial cells formed simple cuboidal epithelia with basally-located nuclei when cultured on collagen gels. The intestinal epithelial nature of the cells was affirmed by the presence of villin on their luminal surface. Ultrastructurally, cells were connected by tight junctions and desmosomes at the subluminal region, and microvilli were projecting on the luminal surface, indicating that the cells in primary culture retained some characteristics of absorptive epithelial cells.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Primary culture of intestinal epithelial cells as a potential model for Toxoplasma gondii enteric cycle studies

2009 ◽  
Vol 104 (6) ◽  
pp. 862-864 ◽  
Author(s):  
Marcos de Assis Moura ◽  
Maria Regina Reis Amendoeira ◽  
Helene Santos Barbosa
Keyword(s):  
Toxoplasma Gondii ◽  
Epithelial Cells ◽  
Primary Culture ◽  
Potential Model ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial

scholarly journals Characterization of conditions for the primary culture of human small intestinal epithelial cells

2001 ◽  
Vol 125 (1) ◽  
pp. 32-40 ◽  
Author(s):  
M. C. Aldhous ◽  
A. N. Shmakov ◽  
J. Bode ◽  
S. Ghosh
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Small Intestinal

Profile of IGF-binding proteins secreted by intestinal epithelial cells changes with differentiation

1994 ◽  
Vol 267 (5) ◽  
pp. G843-G850 ◽  
Author(s):  
S. Oguchi ◽  
W. A. Walker ◽  
I. R. Sanderson
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Epithelial Cells ◽  
Binding Proteins ◽  
Egf Receptor ◽  
Transforming Growth Factor ◽  
Intestinal Epithelial Cells ◽  
Alpha Activity ◽  
Intestinal Epithelial ◽  
Igf I

Previous reports have shown that gastrointestinal epithelial cells produce insulin-like growth factor-binding proteins (IGF-BP), which modulate the actions of IGF. This study aims to examine the relationship between differentiation and IGF-BP secretion by human intestinal epithelial cells and the effect of growth factors on their production. Caco-2 cells were cultured in serum-free media. IGF-BP secretion into the incubation media was analyzed by Western ligand blotting and immunoblotting. Caco-2 cells produced IGF-BP-2, IGF-BP-3, and IGF-BP-4. Secretion of IGF-BP-2 and IGF-BP-3 increased with differentiation, but IGF-BP-4 secretion diminished. The effect of exogenous growth factors on IGF-BP secretion was maximal at earlier stages of differentiation. IGF-I stimulated mainly IGF-BP-3 production, but epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-alpha) stimulated predominantly IGF-BP-4 secretion. Adding an anti-EGF receptor antibody to block autocrine TGF-alpha activity inhibited IGF-BP-4 production but stimulated IGF-BP-2 and IGF-BP-3. In conclusion, the profile of IGF-BP secretion changes with differentiation. IGF-I and EGF (or TGF-alpha) stimulate different types of IGF-BP, with autocrine TGF-alpha activity being a factor affecting IGF-BP production during differentiation.

scholarly journals A pumpless body-on-a-chip model using a primary culture of human intestinal cells and a 3D culture of liver cells

Lab on a Chip ◽  
2018 ◽  
Vol 18 (14) ◽  
pp. 2036-2046 ◽  
Author(s):  
Huanhuan Joyce Chen ◽  
Paula Miller ◽  
Michael L. Shuler
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Intestinal Epithelial Cells ◽  
3D Culture ◽  
Intestinal Cells ◽  
Intestinal Epithelial ◽  
Human Intestinal Epithelial Cells ◽  
Improved Model ◽  
Drug Studies ◽  
Human Intestinal Cells

A pumpless GI–Liver system using primary human intestinal epithelial cells serves as an improved model for drug studies.

Conditionally immortalized intestinal epithelial cells: novel approach for study of differentiated enterocytes

1993 ◽  
Vol 265 (1) ◽  
pp. C266-C278 ◽  
Author(s):  
E. C. Paul ◽  
J. Hochman ◽  
A. Quaroni
Keyword(s):  
Epithelial Cells ◽  
Cell Lines ◽  
Intestinal Epithelial Cells ◽  
Simian Virus 40 ◽  
T Antigen ◽  
Intestinal Epithelial ◽  
Sv40 Large T Antigen ◽  
Antigen Gene ◽  
Fetal Rat ◽  
Metallothionein Promoter

Clonal cell lines have been established from primary fetal rat intestinal epithelial cells by stable transfection with plasmids containing either the simian virus 40 (SV40) large T-antigen gene under the control of the heavy metal inducible metallothionein promoter (pMTWt) or the thermolabile SV40 T-antigen gene under the control of the SV40 early promoter (pZipSVtsa58). pMTWt-transfected cells produced sufficient T-antigen to allow them to proliferate both when the metallothionein promoter was induced and uninduced. No differences were observed in the pattern of intestinal epithelial markers expressed when the cells were cultured in the presence or absence of inducing agent (zinc). In contrast, fetal rat intestinal epithelial cells transfected with pZipSVtsa58 were immortalized conditionally; cells proliferated at 32 degrees C but ceased to proliferate between 48 and 72 h of culture at 39 degrees C. Four of these cell lines were characterized in detail; they showed microvilli and tight junctions as well as dome formation and expressed functional and biochemical markers of intestinal epithelial cells, including keratins 8, 19, and 21, aminopeptidase N, and dipeptidyl peptidase IV. One cell line, 2/4/A1, expressed in addition a low level of lactase and sucrase-isomaltase. The amount and/or activity of some of these markers changed during the switch from the proliferative to the nonproliferative state (switch from culture at 32 to 39 degrees C), resulting in a more differentiated phenotype and mimicking similar changes taking place during intestinal epithelial cell differentiation in vivo.

scholarly journals PPAR? agonists inhibit cell growth and suppress the expression of cyclin D1 and EGF-like growth factors in ras-transformed rat intestinal epithelial cells

2001 ◽  
Vol 94 (3) ◽  
pp. 335-342 ◽  
Author(s):  
Shinji Kitamura ◽  
Yoshiji Miyazaki ◽  
Shintaro Hiraoka ◽  
Yutaka Nagasawa ◽  
Miyuki Toyota ◽  
...  
Keyword(s):  
Growth Factors ◽  
Cell Growth ◽  
Epithelial Cells ◽  
Cyclin D1 ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Inhibit Cell Growth ◽  
Ppar Agonists

Isolation and primary culture of chicken intestinal epithelial cells retaining normal in vivo-like morphology

1993 ◽  
Vol 15 (1) ◽  
pp. 15-18 ◽  
Author(s):  
D. J. Caldwell ◽  
R. E. Droleskey ◽  
M. H. Elissalde ◽  
M. H. Kogut ◽  
J. R. DeLoach ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial
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