In vitro analysis of the melanoma/endothelium interaction increasing the release of soluble intercellular adhesion molecule 1 by endothelial cells

1999 ◽  
Vol 48 (2-3) ◽  
pp. 132-138 ◽  
Author(s):  
Ester Fonsatti ◽  
Elda Lamaj ◽  
Sandra Coral ◽  
Luca Sigalotti ◽  
Gianpaolo Nardi ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Adhesion Molecule ◽  
Intercellular Adhesion ◽  
Intercellular Adhesion Molecule ◽  
Intercellular Adhesion Molecule 1 ◽  
Soluble Intercellular Adhesion Molecule ◽  
Vitro Analysis ◽  
In Vitro Analysis

In vitro and in situ intercellular adhesion molecule-1 (ICAM-1) expression by endothelial cells lining a polyester fabric

Biomaterials ◽  
1999 ◽  
Vol 20 (3) ◽  
pp. 241-251 ◽  
Author(s):  
M. Rémy ◽  
N. Valli ◽  
D. Brethes ◽  
C. Labrugère ◽  
M.C. Porté-Durrieu ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Adhesion Molecule ◽  
Polyester Fabric ◽  
Intercellular Adhesion ◽  
Intercellular Adhesion Molecule ◽  
Intercellular Adhesion Molecule 1

scholarly journals The Protective Effects of Preconditioning on Cerebral Endothelial Cells in Vitro

2003 ◽  
Vol 23 (11) ◽  
pp. 1348-1355 ◽  
Author(s):  
Anuska V Andjelkovic ◽  
Svetlana M Stamatovic ◽  
Richard F Keep
Keyword(s):  
Endothelial Cells ◽  
Lactate Dehydrogenase ◽  
Mouse Brain ◽  
Adhesion Molecule ◽  
Intercellular Adhesion ◽  
Intercellular Adhesion Molecule ◽  
Intercellular Adhesion Molecule 1 ◽  
Cerebral Endothelial Cells ◽  
Lactate Dehydrogenase Release

Ischemic preconditioning (PC) can markedly reduce ensuing ischemic damage. Although most attention has focused on the neuronal effects of PC, the authors have recently shown that ischemic PC reduces ischemia-induced cerebrovascular damage. In vivo, it is difficult to ascertain whether this is a direct cerebrovascular effect of PC. This study, therefore, examined whether cerebral endothelial cells can be preconditioned in vitro in the absence of other cell types. Experiments were performed on an immortalized mouse brain endothelial cell line or primary cultures of mouse brain microvessel endothelial cells. Cells were exposed to oxygen glucose deprivation (OGD) of either short duration, as a PC stimulus, or a long duration (5 hours) with or without reoxygenation to induce endothelial damage. Endothelial injury was assessed by measuring lactate dehydrogenase release and the expression of intercellular adhesion molecule-1 at the protein and mRNA levels. Experiments indicated that 1 hour of OGD was the optimal PC stimuli and that a 1 or 3 day interval was the optimal time interval between the PC stimulus and the injurious event. Preconditioned cells had less lactate dehydrogenase release during OGD (± reoxygenation) and reduced intercellular adhesion molecule-1 expression after OGD with reoxygenation. This study shows that cerebral endothelial cells can be directly preconditioned. The importance of this phenomenon in the overall effects of PC on the brain remains to be elucidated. Understanding the protective mechanisms elicited by PC may give insight into how to prevent ischemia-induced vascular damage (e.g., hemorrhagic transformation).

scholarly journals Lycopene Inhibit IMQ-Induced Psoriasis-Like Inflammation by Inhibiting ICAM-1 Production in Mice

Polymers ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 1521
Author(s):  
Chun-Ming Shih ◽  
Chi-Kun Hsieh ◽  
Chien-Yu Huang ◽  
Chun-Yao Huang ◽  
Kuo-Hsien Wang ◽  
...  
Keyword(s):  
Adhesion Molecules ◽  
Inflammatory Responses ◽  
Critical Role ◽  
Intercellular Adhesion ◽  
Intercellular Adhesion Molecule ◽  
Intercellular Adhesion Molecule 1 ◽  
Intercellular Adhesion Molecules ◽  
Vitro Analysis ◽  
In Vitro Analysis

Lycopene is the most abundant carotenoid in tomatoes, which has been identified to have the properties of anti-inflammation in addition to the capability to inhibit the expression of adhesion molecules. Intercellular adhesion molecules play a critical role in the pathogenesis of psoriasis. Here, we report that the topical use of a lycopene decreased imiquimod (IMQ)-induced psoriasis-like inflammatory responses, the progress of which was based on adhesion molecules. In vitro analysis showed that lycopene decreased keratinocyte and monocyte adhesion. Evidence suggests that intercellular adhesion molecule-1 (ICAM-1) is a main mediator of psoriasis pathogenesis. Therefore, it will be interesting to investigate the factors that contribute to the lycopene-mediated inhibition of ICAM-1 expression in psoriasis. We expect that lycopene will with potential value in the treatment of psoriasis.

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