The Fate of Helicobacter pylori Phagocytized by Acanthamoeba polyphaga Demonstrated by Fluorescent In Situ Hybridization and Quantitative Polymerization Chain Reaction Tests

2012 ◽  
Vol 65 (6) ◽  
pp. 805-812 ◽  
Author(s):  
Charlotte D. Smith ◽  
Nicholas J. Ashbolt
Keyword(s):  
Helicobacter Pylori ◽  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Acanthamoeba Polyphaga ◽  
Chain Reaction ◽  
Polymerization Chain

scholarly journals Molecular detection of Helicobacter pylori in a large Mediterranean river, by direct viable count fluorescent in situ hybridization (DVC-FISH)

2014 ◽  
Vol 12 (4) ◽  
pp. 868-873 ◽  
Author(s):  
Ilias Tirodimos ◽  
Mattheos Bobos ◽  
Evangelos Kazakos ◽  
Anna-Bettina Haidich ◽  
Theodore Dardavessis ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Potable Water ◽  
Molecular Detection ◽  
Viable Count ◽  
Potential Health ◽  
Viable Cells ◽  
H Pylori

Although the precise route and mode of transmission of Helicobacter pylori are still unclear, molecular methods have been applied for the detection of H. pylori in environmental samples. In this study, we used the direct viable count fluorescent in situ hybridization (DVC-FISH) method to detect viable cells of H. pylori in the River Aliakmon, Greece. This is the longest river in Greece, and provides potable water in metropolitan areas. H. pylori showed positive detection for 23 out of 48 water samples (47.9%), while no seasonal variation was found and no correlation was observed between the presence of H. pylori and indicators of fecal contamination. Our findings strengthen the evidence that H. pylori is waterborne while its presence adds to the potential health hazards of the River Aliakmon.

HCR RNA-FISH protocol for the whole-mount brains of Drosophila and other insects v1

2021 ◽  
Author(s):  
Amanda A. G. Ferreira ◽  
Bogdan Sieriebriennikov ◽  
Hunter Whitbeck
Keyword(s):  
Drosophila Melanogaster ◽  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Alexa Fluor ◽  
Rna Fish ◽  
Whole Mount

This is a protocol to perform RNA fluorescent in situ hybridization (RNA-FISH) using hybridization chain reaction (HCR) on whole-mount samples of the brains of the fly Drosophila melanogaster and other insects, e.g. the jumping ant Harpegnathos saltator. Probes and HCR reagents are purchased from Molecular Instruments. This protocol is loosely based on the "generic sample in solution" protocol published by Molecular Instruments. Our modifications include the description of fixation conditions, counterstaining by Hoechst, and altered washes. Additionally, we use larger concentrations of probes and hairpins following the protocol described by Younger, Herre et al. 2020. We have successfully employed this protocol to stain insect brains with up to 4 different probe sets simultaneously (hairpins conjugated with Alexa Fluor 488, 546, 496, and 647).

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