Biocontrol Potential of Trichoderma afroharzianum TM24 Against Grey Mould on Tomato Plants

Author(s):  
Juan Zhao ◽  
Ting Liu ◽  
Dianpeng Zhang ◽  
Huiling Wu ◽  
Taotao Zhang ◽  
...  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Qassim Esmaeel ◽  
Cédric Jacquard ◽  
Lisa Sanchez ◽  
Christophe Clément ◽  
Essaid Ait Barka

Abstract Plant-associated Burkholderia spp. have been shown to offer a promising alternative method that may address concerns with ecological issue associated with pesticide overuse in agriculture. However to date, little work has studied the role of Burkholderia species as biocontrol agents for grapevine pathogens. To this end, two Burkholderia strains, BE17 and BE24 isolated from the maize rhizosphere in France, were investigated to determine their biocontrol potential and their ability to induce systemic resistance against grey mould disease in grapevine. Results showed the capacity of both strains to inhibit spore germination and mycelium growth of Botrytis cinerea. Experimental inoculation with BE17 and BE24 showed a significant protection of bacterized-plantlets against grey mould compared to the non-bacterized control. BE17 and BE24-bacterized plants accumulated more reactive oxygen species and an increased callose deposition was observed in leaves of bacterized plantlets compared to the control plantlets. In bacterized plants, gene expression analysis subsequent to B. cinerea challenge showed that strains BE17 and BE24 significantly increased the relative transcript level of pathogenesis-related (PR) proteins PR5 and PR10, two markers involved in the Salicylic acid (SA)-signaling pathway. Furthermore, in silico analysis of strains revealed the presence of genes involved in plant growth promotion and biocontrol highlighting the attractiveness of these strains for sustainable agricultural applications.


2019 ◽  
Vol 156 (1) ◽  
pp. 237-243 ◽  
Author(s):  
Jolanta Kowalska ◽  
Józef Tyburski ◽  
Joanna Krzymińska ◽  
Magdalena Jakubowska

AbstractIn this study, the effects of organic powder of Cinnamomum zeylanicum on the development of Botrytis cinerea and its influence on tomato plants were evaluated. The cinnamon bark powder and its water suspensions and filtrates were used at 0.5 and 1% rates. After 6 days of the start of an in vitro experiment the mycelium growth was inhibited by both 0.5 and 1% cinnamon water filtrates - to a greater degree in the case of the higher concentration, by 54.4 and 81.4%, respectively. Spraying with cinnamon water filtrates positively influenced the growth of plants both in the greenhouse and the field. Antifungal activity of cinnamon was proved in the greenhouse tests - the disease symptoms of grey mould on infected tomato plants decreased. The fresh weight of non-inoculated tomato plants treated with cinnamon filtrates was significantly higher than control plants (17.17 g compared to 12.83 g) showing a stimulating effect of cinnamon filtrates. In the case of inoculated plants due to treatment, their weight increased from 7.83 to 10.50 g. In the field experiment, tomato plants sprayed six times with cinnamon were better developed than the control plants. The most significant effect was observed for Hamlet variety - the mean number of leaves was higher by 27.3% and the mean number of branches by 19.7% compared to the untreated control plants. Thus it was proved that cinnamon powder has potential to inhibit B. cinerea growth and also has a stimulating effect for tomato plants.


Author(s):  
Manigundan Kaari ◽  
Jerrine Joseph ◽  
Radhakrishnan Manikkam ◽  
Ayswarya Sreenivasan ◽  
Gopikrishnan Venugopal ◽  
...  

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 817
Author(s):  
Pablo Vargas ◽  
Lien Bosmans ◽  
Stefan Van Kerckhove ◽  
Bart Van Calenberge ◽  
Jos M. Raaijmakers ◽  
...  

Hairy root disease (HRD) caused by rhizogenic Agrobacterium biovar 1 strains affect tomato, cucumber, eggplant, and bell pepper grown in hydroponic greenhouses and can cause considerable yield losses worldwide. Recently, Paenibacillus xylanexedens strains (ST15.15/027 and AD117) with antagonistic activity against rhizogenic agrobacteria were identified. In this study, we present results of greenhouse trials of two consecutive growing seasons (2019 and 2020) to examine the potential of these two biocontrol organisms (BCOs) under practical conditions. BCO-treatment at a 107 colony forming units (CFU)/mL density resulted in a considerable reduction of the HRD infestation rate, confirming the biocontrol potential of the two P. xylanexedens strains. Results revealed that a single BCO strain (ST15.15/027) performed equally well as the mixed inoculum of both strains. The same level of biocontrol activity was even achieved when the BCO inoculum density was reduced to 105 CFU/mL. qPCR analysis further showed that Paenibacillus was still present in rockwool substrate near the end of both trials, indicating that they persist well in a rockwool environment and that application at the start of the trial is sufficient to protect tomato plants until the end of the trial. Altogether, these results are highly valuable for further optimization and exploitation of P. xylanexedens as a biocontrol product for the control of HRD in hydroponic greenhouses.


2016 ◽  
Vol 32 (5) ◽  
pp. 469-480 ◽  
Author(s):  
Jeum Kyu Hong ◽  
Hyeon Ji Kim ◽  
Heesoo Jung ◽  
Hye Ji Yang ◽  
Do Hoon Kim ◽  
...  

2015 ◽  
Vol 5 (1) ◽  
pp. 513-522
Author(s):  
Jean-Claude N'ZI ◽  
Lassina FONDIO ◽  
Mako Francois De Paul N’GBESSO ◽  
Andé Hortense DJIDJI ◽  
Christophe KOUAME

Thirty accessions of tomato including twenty eight introduced accessions from The World Vegetable Center-AVRDC and as controls, two commercial varieties Mongal and Calinago, were assessed for agronomic performances at the Experimentation and Production Station of Angud dou of the National Agronomic Research Centre (CNRA) located in the South of Cote d Ivoire. The trial was arranged in a randomized block with three replications. The following parameters were determined at vegetative development stage: plant height at flowering stage, susceptibility of accessions to diseases, day to 50% flowering and day of first harvest, production duration, fruit length, fruit diameter, total number of fruits, number of fruits per plant, potential yield, net yield and fruit damage rate. Results showed that the commercial variety Mongal, with a potential yield of 15.9 and a net yield of 13.1 t ha-1, was the most productive. All the introduced accessions from AVRDC recorded the lowest potential yields from 2.2 to 9.7 t ha-1, and net yields from 1.7 to 8.6 t ha-1. In addition, accessions WVCT8, FMTT847 and WVCT13 were severely infested by bacterial wilt. The reduction of the net yield of tomato accessions resulted in the high fruit damage rates. For the future tomato breeding work, it would be appropriate to introduce into the trials bacterial diseases tolerant varieties. Moreover, some studies could be undertaken to determine the nature of the bacteria involved in the plant wilting and to find out the causal agent of the tomato plants burning at the fructification stage reducing the harvest duration.


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