Molecular Characterization of the Host-Adapted Pathogen Verticillium longisporum on the Basis of a Group-I Intron Found in the Nuclear SSU-rRNA Gene

2001 ◽  
Vol 42 (3) ◽  
pp. 217-224 ◽  
Author(s):  
Vassiliki K. Karapapa ◽  
Milton A. Typas
Keyword(s):  
Molecular Characterization ◽  
Group I Intron ◽  
Rrna Gene ◽  
Ssu Rrna ◽  
Ssu Rrna Gene ◽  
Verticillium Longisporum ◽  
Group I

scholarly journals Prevalence and molecular characterization of Cryptosporidium spp. in Père David’s deer (Elaphurus davidianus) in Jiangsu, China

2020 ◽  
Vol 29 (2) ◽  
Author(s):  
Si-Yang Huang ◽  
Yi-Min Fan ◽  
Yi Yang ◽  
Yi-Jun Ren ◽  
Jing-Zhi Gong ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Small Subunit ◽  
Basic Knowledge ◽  
Rrna Gene ◽  
Ssu Rrna ◽  
Ssu Rrna Gene ◽  
Cryptosporidium Spp ◽  
Père David’S Deer ◽  
Rrna Gene Sequencing

Abstract Cryptosporidium is a zoonotic parasite that causes diarrhea in a broad range of animals, including deer. Little is known about the prevalence and genotype of Cryptosporidium spp. in Père David’s deer. In this study, 137 fecal samples from Père David’s deer were collected between July 2017 and August 2018 in the Dafeng Reserve and analyzed for Cryptosporidium spp. by nested-PCR based on the small subunit ribosomal RNA (SSU rRNA) gene, followed by sequence analyses to determine the species. The 60 kDa glycoprotein (gp60) gene was used to characterize Cryptosporidium spp. Among 137 samples, 2 (1.46%) were positive for Cryptosporidium spp. according to SSU rRNA gene sequencing results. Both samples belonged to the Cryptosporidium deer genotype, with two nucleotide deletions and one nucleotide substitution. The prevalence data and molecular characterization of this study provide basic knowledge for controlling and preventing Cryptosporidium infections in Père David’s deer in this area.

scholarly journals “Cryptic” group-I introns in the nuclear SSU-rRNA gene of Verticillium dahliae

2013 ◽  
Vol 60 (3) ◽  
pp. 135-148 ◽  
Author(s):  
Ioannis A. Papaioannou ◽  
Chrysoula D. Dimopoulou ◽  
Milton A. Typas
Keyword(s):  
Verticillium Dahliae ◽  
Rrna Gene ◽  
Group I Introns ◽  
Ssu Rrna ◽  
Ssu Rrna Gene ◽  
Group I

scholarly journals Prevalence and Genetic Characterization of Cryptosporidium Infection in Java Sparrows (Lonchura oryzivora) in Northern China

2017 ◽  
Vol 2017 ◽  
pp. 1-4 ◽  
Author(s):  
Qiu-Xia Yao ◽  
Xiao-Xuan Zhang ◽  
Kai Chen ◽  
Jian-Gang Ma ◽  
Wen-Bin Zheng ◽  
...  
Keyword(s):  
Pcr Amplification ◽  
Northern China ◽  
Small Subunit ◽  
Rrna Gene ◽  
Ssu Rrna ◽  
Ssu Rrna Gene ◽  
Wide Range ◽  
Baseline Information ◽  
And Control

Cryptosporidiosis is a cosmopolitan parasitosis that affects a wide range of hosts including birds. As information concerning Cryptosporidium in birds is limited, the present study examined the prevalence and genotypes of Cryptosporidium in Java sparrows in Beijing and Shangqiu, northern China. Three hundred and fifty fecal samples were collected from Java sparrows (Lonchura oryzivora, 225 white Java sparrows and 125 gray Java sparrows) in Beijing and Shangqiu in October 2015, and the samples were examined by PCR amplification of the small subunit ribosomal RNA (SSU rRNA) gene. The overall Cryptosporidium prevalence is 13.42% (47/350), with 16.44% (37/225) in white Java sparrows and 8.00% (10/125) in gray Java sparrows. Cryptosporidium prevalence was 9.82% (16/163) in Java sparrows from Beijing and 16.58% (31/187) in Java sparrows from Shangqiu. The prevalence of Cryptosporidium in females and males was 40.63% (26/64) and 7.34% (21/286), respectively. The Cryptosporidium prevalence in Java sparrows of different ages varied from 10.47% to 16.33%. Sequence analysis of the SSU rRNA gene revealed that all the samples represented C. baileyi. This is the first report of Cryptosporidium in gray Java sparrows in China, which extend the host range for C. baileyi. These results provide baseline information for further studies of molecular epidemiology and control of Cryptosporidium infection in poultry in China.

scholarly journals Characterization of a single group I intron in the 18S rRNA gene of the pathogenic fungusHistoplasma capsulatum

1998 ◽  
Vol 36 (4) ◽  
pp. 205-212 ◽  
Author(s):  
Brent A. Lasker ◽  
Gwen W. Smith ◽  
George S. Kobayashi ◽  
Anne M. Whitney ◽  
Leonard W. Mayer
Keyword(s):  
18S Rrna ◽  
Group I Intron ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Single Group ◽  
Group I

Characterization of a single group I intron in the 18S rRNA gene of the pathogenic fungus Histoplasma capsulatum

1998 ◽  
Vol 36 (4) ◽  
pp. 205-212
Author(s):  
BRENT A. LASKER ◽  
GWEN W. SMITH ◽  
GEORGE S. KOBAYASHI ◽  
ANNE M. WHITNEY ◽  
LEONARD W. MAYER
Keyword(s):  
18S Rrna ◽  
Histoplasma Capsulatum ◽  
Pathogenic Fungus ◽  
Group I Intron ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Single Group ◽  
Group I

Multiple origins of fungal group I introns located in the same position of nuclear SSU rRNA gene

1998 ◽  
Vol 46 (4) ◽  
pp. 442-448 ◽  
Author(s):  
Hiromi Nishida ◽  
Yoshito Tajiri ◽  
Junta Sugiyama
Keyword(s):  
Rrna Gene ◽  
Group I Introns ◽  
Ssu Rrna ◽  
Ssu Rrna Gene ◽  
Multiple Origins ◽  
Group I

scholarly journals Molecular Characterization of Blastocystis sp. in Camelus bactrianus in Northwestern China

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3016
Author(s):  
Xin Yang ◽  
Yunhui Li ◽  
Yuxin Wang ◽  
Junwei Wang ◽  
Peng Lai ◽  
...  
Keyword(s):  
Northwestern China ◽  
Rrna Gene ◽  
Ssu Rrna ◽  
Infection Rates ◽  
Ssu Rrna Gene ◽  
Worldwide Distribution ◽  
Total Prevalence ◽  
Faecal Samples ◽  
Blastocystis Sp

Blastocystis sp. is an important zoonotic protist in humans and various animals with worldwide distribution. However, there have been no data on the occurrence of Blastocystis sp. in C. bactrianus, an important economic animal in northwestern China. In the present study, a PCR-sequencing tool based on the SSU rRNA gene was applied to investigate the prevalence and genetic diversity of Blastocystis sp. in 638 faecal samples from C. bactrianus in 21 sampling sites within three main breeding areas (Gansu, Inner Mongolia and Xinjiang) in northwestern China. The total prevalence of Blastocystis sp. was 21.8% (139/638) in C. bactrianus, with the infection rates of 29.5% (18/61), 50.0% (14/28) and 19.5% (107/549) for animals aged <2 years, 2–6 years and >6 years, respectively. Significant differences in prevalence were detected among C. bactrianus from three geographic areas (χ2 = 19.972, df = 2, p < 0.001) and all sampling sites (χ2 = 104.154, df = 20, p < 0.001). A total of 16 of 21 sampling sites were positive for Blastocystis sp., with the prevalence ranging from 7.7% to 70.6%. Sequence analysis of the SSU rRNA gene identified eight subtypes in C. bactrianus in the present study, including seven animal adapted subtypes (ST10, ST14, ST21, ST24, ST25, ST26 and ST30) and one potentially novel subtype, with ST10 being the dominant one. To the best of our knowledge, this study provides the first insight for the occurrence and genetic make-up of Blastocystis sp. in C. bactrianus and contributes to the understanding of the transmission of Blastocystis infection in C. bactrianus in China.

Characterization of a single group I intron in the 18S rRNA gene of the pathogenic fungus Histoplasma capsulatum

2008 ◽  
Vol 36 (4) ◽  
pp. 205-212
Author(s):  
BRENT A. LASKER ◽  
GWEN W. SMITH ◽  
GEORGE S. KOBAYASHI ◽  
ANNE M. WHITNEY ◽  
LEONARD W. MAYER
Keyword(s):  
18S Rrna ◽  
Histoplasma Capsulatum ◽  
Pathogenic Fungus ◽  
Group I Intron ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Single Group ◽  
Group I
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