scholarly journals Characterization of morphology and resistance to Blumeria graminis of winter triticale monosomic addition lines with chromosome 2D of Aegilops tauschii

2016 ◽  
Vol 35 (10) ◽  
pp. 2125-2135 ◽  
Author(s):  
M. Majka ◽  
M. Kwiatek ◽  
J. Belter ◽  
H. Wiśniewska
Genome ◽  
1993 ◽  
Vol 36 (4) ◽  
pp. 750-761 ◽  
Author(s):  
A. S. Reddy ◽  
M. C. Kiefer-Meyer ◽  
M. Delseny

Two groups of tandemly repeated DNA fragments have been isolated and cloned from the Oryza officinalis genome (W 1278). These fragments have been sequenced and are 374 and 367 bp long. They were compared with an element previously isolated from another O. officinalis accession. They are 76 and 92% homologous to the latter and show 78% homology between themselves. The two types of elements are interspersed within large blocks of tandemly organized units. Their copy number is in the range of 200 000 units. This sequence was present only in wild rice accessions with a CC genome. However, this apparent specificity is even more restricted, since it was absent from some O. officinalis accessions and from the allotetraploid species of the CCDD type. Use of digestions with 4-bp restriction enzyme cutters and analysis on denaturing polyacrylamide gels revealed polymorphism that might be used to map the repeats in the genome as well as to establish the relationship between accessions. Finally, hybridization of the repeated sequence to DNA prepared from the eight available O. sativa – O. officinalis monosomic addition lines revealed that the elements are located on two alien chromosomes. This suggests that these elements have at least some restricted chromosome specificity.Key words: satellite DNA, rice, monosomic addition lines, RFLP, evolution.


2009 ◽  
Vol 59 (2) ◽  
pp. 109-118 ◽  
Author(s):  
Michiko Akaba ◽  
Yukio Kaneko ◽  
Youichi Ito ◽  
Yoshimi Nakata ◽  
Sang Woo Bang ◽  
...  

PLoS ONE ◽  
2013 ◽  
Vol 8 (7) ◽  
pp. e70483 ◽  
Author(s):  
Shulan Fu ◽  
Manyu Yang ◽  
Yunyan Fei ◽  
Feiquan Tan ◽  
Zhenglong Ren ◽  
...  

2003 ◽  
Vol 122 (5) ◽  
pp. 450-452 ◽  
Author(s):  
Y. Kaneko ◽  
S. W. Bang ◽  
J. Torii-Abe ◽  
R. B. Eduardo ◽  
Y. Matsuzawa ◽  
...  

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e54057 ◽  
Author(s):  
Shulan Fu ◽  
Chuanfei Sun ◽  
Manyu Yang ◽  
Yunyan Fei ◽  
Feiqun Tan ◽  
...  

Genome ◽  
2016 ◽  
Vol 59 (3) ◽  
pp. 159-165 ◽  
Author(s):  
Ling Qiu ◽  
Zong-xiang Tang ◽  
Meng Li ◽  
Shu-lan Fu

PCR-based rye (Secale cereale L.) chromosome-specific markers can contribute to the effective utilization of elite genes of rye in wheat (Triticum aestivum L.) breeding programs. In the present study, 578 new PCR-based rye-specific markers have been developed by using specific length amplified fragment sequencing (SLAF-seq) technology, and 76 markers displayed different polymorphism among rye Kustro, Imperial, and King II. A total of 427 and 387 markers were, respectively, located on individual chromosomes and chromosome arms of Kustro by using a set of wheat–rye monosomic addition lines and 13 monotelosomic addition lines, which were derived from T. aestivum L. ‘Mianyang11’ × S. cereale L. ‘Kustro’. In addition, two sets of wheat–rye disomic addition lines, which were derived from T. aestivum L. var. Chinese Spring × S. cereale L. var. Imperial and T. aestivum L. ‘Holdfast’ × S. cereale L. var. King II, were used to test the chromosomal specificity of the 427 markers. The chromosomal locations of 281 markers were consistent among the three sets of wheat–rye addition lines. The markers developed in this study can be used to identify a given segment of rye chromosomes in wheat background and accelerate the utilization of elite genes on rye chromosomes in wheat breeding programs.


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