Characterization of lipid droplets from a Taxus media cell suspension and their potential involvement in trafficking and secretion of paclitaxel

2022 ◽  
Author(s):  
Abdulsamie Hanano ◽  
Edgar Perez-Matas ◽  
Mouhnad Shaban ◽  
Rosa M. Cusido ◽  
Denis J. Murphy
Keyword(s):  
Cell Suspension ◽  
Lipid Droplets ◽  
Taxus Media

Production and characterization of recombinant human acid α-glucosidase in transgenic rice cell suspension culture

2016 ◽  
Vol 226 ◽  
pp. 44-53 ◽  
Author(s):  
Jae-Wan Jung ◽  
Nan-Sun Kim ◽  
Seon-Hui Jang ◽  
Yun-Ji Shin ◽  
Moon-Sik Yang
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Transgenic Rice ◽  
Cell Suspension Culture ◽  
Rice Cell Suspension Culture ◽  
Human Acid

Production and characterization of human CTLA4Ig expressed in transgenic rice cell suspension cultures

2007 ◽  
Vol 51 (2) ◽  
pp. 293-302 ◽  
Author(s):  
Song-Jae Lee ◽  
Cheon-Ik Park ◽  
Mi-Young Park ◽  
Hahn-Sun Jung ◽  
Wuk-Sang Ryu ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Transgenic Rice ◽  
Cell Suspension Cultures ◽  
Suspension Cultures

Characterization of emulsions and lipolysis of dietary lipids in the human stomach

1994 ◽  
Vol 266 (3) ◽  
pp. G372-G381 ◽  
Author(s):  
M. Armand ◽  
P. Borel ◽  
C. Dubois ◽  
M. Senft ◽  
J. Peyrot ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Droplets ◽  
Present Finding ◽  
Droplet Diameter ◽  
Human Stomach ◽  
Dietary Lipids ◽  
Gastric Lipase ◽  
A Minor ◽  
First Time

Fasting subjects were intragastrically intubated and received a coarsely emulsified test meal. Gastric aspirates were collected after 1, 2, 3, and 4 h. During digestion in the stomach, unemulsified lipids (> or = 100 microns) represented a minor fraction. A significant amount of the large 70- to 100-microns lipid droplets disappeared, and fine 1- to 10-microns droplets were generated. The median lipid droplet diameter significantly decreased (21.9 vs. 52.9 microns) after 1 h and kept intermediate values for longer periods of time. The emulsion surface area was 100-120 m2/l and was basically provided by 1- to 100-microns droplets. Lipolysis catalyzed by gastric lipase primarily occurred within the first hour of digestion (11.9%). Smaller droplets were enriched in triglyceride lipolytic products. The free fatty acid concentrations were in the range of 5.6-8.2 mM over 1-4 h. The present finding demonstrates for the first time that in the human stomach most dietary lipids are present in the form of emulsified droplets, in the range of 20-40 microns, and that gastric lipolysis can help to increase emulsification in the stomach.

Establishment of cell suspension cultures of yew (Taxus × media Rehd. and assessment of their genomic stability

2005 ◽  
Vol 41 (3) ◽  
pp. 338-343 ◽  
Author(s):  
Špela Baebler ◽  
Matjaž Hren ◽  
Marjana Camloh ◽  
Maja Ravnikar ◽  
Borut Bohanec ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Genomic Stability ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Taxus Media

Characterization of Volatile Constituents from Photomixotrophic Cell Suspension Cultures of Ruta graveolens

1984 ◽  
Vol 39 (6) ◽  
pp. 525-530 ◽  
Author(s):  
Friednch Drawert ◽  
Ralf G. Berger ◽  
Rolf Godelmann ◽  
Susanne Collin ◽  
Wolfgang Barz
Keyword(s):  
Acetic Acid ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Callus Cultures ◽  
Suspension Cultures ◽  
Ruta Graveolens ◽  
Straight Chain ◽  
Methylbutyric Acid ◽  
Aliphatic Esters

Photomixotrophic cell suspension cultures of Ruta graveolens were qualitatively and quantita­tively analyzed by gaschromatography and mass spectroscopy for volatile compounds. The terpenoid hydrocarbons geijerene and pregeijerene, the C9-C13 methylketones and a series of aliphatic esters, respectively, were found as main constituents. The esters consisted of acetic acid, 2-methylbutyric acid and 3-methylbutyric acid which were esterified with straight chain or branched C8-C11 alcohols. The data are discussed in comparison to previous studies on callus cultures.

scholarly journals Quantitative imaging of lipid droplets in single cells

The Analyst ◽  
2019 ◽  
Vol 144 (3) ◽  
pp. 753-765 ◽  
Author(s):  
Anushka Gupta ◽  
Gabriel F. Dorlhiac ◽  
Aaron M. Streets
Keyword(s):  
Lipid Droplets ◽  
Single Cells ◽  
Quantitative Imaging ◽  
Cell Level ◽  
Spatial Characterization ◽  
Coherent Raman Scattering ◽  
Coherent Raman ◽  
Computational Image Analysis ◽  
Non Destructive

Non-destructive spatial characterization of lipid droplets using coherent Raman scattering microscopy and computational image analysis algorithms at the single-cell level.

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