Condensed tannin and anthocyanin production in Vitis vinifera cell suspension cultures

1996 ◽  
Vol 15 (10) ◽  
pp. 762-765 ◽  
Author(s):  
A. Decendit ◽  
J.M. M�rillon
Keyword(s):  
Vitis Vinifera ◽  
Cell Suspension ◽  
Condensed Tannin ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Anthocyanin Production

Regulation of polyphenol production in Vitis vinifera cell suspension cultures by sugars

1998 ◽  
Vol 17 (12) ◽  
pp. 946-950 ◽  
Author(s):  
F. Larronde ◽  
S. Krisa ◽  
A. Decendit ◽  
C. Chèze ◽  
G. Deffieux ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures

scholarly journals STUDYING ON GROWTH OF STRAWBERRY (FRAGARIA ANANASSA L.) CELL SUSPENSION CULTURES FOR ANTHOCYANIN PRODUCTION

2012 ◽  
Vol 15 (2) ◽  
pp. 69-77
Author(s):  
Tram Thi My Pham ◽  
Tien Thi Thuy Le
Keyword(s):  
Cell Suspension ◽  
Light Condition ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Differential Method ◽  
Ms Medium ◽  
Initial Inoculum ◽  
Anthocyanin Production ◽  
Better Than

Cell suspension cultures were initated from calli derived from in vitro strawberry leaves on MS medium (Murashige and Skoog, 1962) supplemented with 30 g/l sucrose, 1.0 mg/l 2,4-D and 0.3 mg/l kinetin. There were many factors affected on cell suspension cultures growth (it was found that …). Cell suspension cultures grew better on MS medium with 30 g/l sucrose. 1 g (fresh weight) of cells in 20 ml of medium was the best initial inoculum cell density for cell suspension cultures to grow. A shaking speed of 100 rpm on rotary shaker was suitable for the cells. The growth of cell suspension in dark was better than that under light condition. Anthocyanin in the cells was determined by pH differential method.

Biosynthese von Cyanidin in Zellsuspensionskulturen von Haplopappus gracilis / Biosynthesis of Cyanidin in Cell Suspension Cultures of Haplopappus gracilis

1971 ◽  
Vol 26 (6) ◽  
pp. 581-585 ◽  
Author(s):  
Hansjörg Fritsch ◽  
Klaus Hahlbrock ◽  
Hans Grisebach
Keyword(s):  
Cell Suspension ◽  
Phenylalanine Ammonia Lyase ◽  
Cell Suspension Cultures ◽  
Maximal Activity ◽  
Suspension Cultures ◽  
Feeding Time ◽  
Haplopappus Gracilis ◽  
Time Required ◽  
Anthocyanin Production

Earlier work with buckwheat seedlings showed that dihydrokaempferol (3,5,7,4′-tetrahydroxyflavanone) (1) is a good precursor for cyanidin and quercetin. Since cell suspension cultures have several advantages for the quantitative comparison of the incorporation of a radioactive precursor into a product, the role of 1 in the biosynthesis of cyanidin was reinvestigated with cell cultures of Haplopappus gracilis. These cultures show good production of cyanidin-glycosides in blue light in a medium containing sucrose, 2,4-D and Fe-EDTA. Maximum anthocyanin production and maximal activity of phenylalanine ammonia-lyase is reached about 60 — 70 hrs. after the onset of illumination. The incorporation of 1 and of phenylalanine and 4,2′,4′,6′-tetrahydroxychalcone into cyanidin was compared under identical conditions after 1, 3 and 6 hrs. feeding time. 1 prooved to be by far the best precursor for cyanidin. The feeding time required was much shorter and the incorporation rates were much higher than in experiments with seedlings.

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