Cuticular Analysis - a method to distinguish the leaves of Pinus sylvestris L. (Scots Pine) from those of Pinus mugo s. str. (Dwarf Mountain-pine)

2002 ◽  
Vol 11 (3) ◽  
pp. 241-246 ◽  
Author(s):  
Dana Stružková

Author(s):  
H. F. Kelko ◽  
M. A. Slesarenka ◽  
R. I. Karaneuski ◽  
U. I. Torchyk

The influence of various concentrations of chemical mutagens on the seed germination, the growth and manifestation of decorative traits in the seed offspring of mountain pine (Pinus mugo Turra), Scots pine (Pinus sylvesrtis L.), and Scots pine “witch’s broom” (“WB”) is assessed. A 0.1 % aqueous solution of colchicine, as well as 0.1, 0.5, and 1.0 % aqueous solutions of dimethyl sulfate (DMS) was used as mutagens. The species-specificity of the influence of chemical mutagens on the seed germination was established. Thus, the treatment of mountain pine seeds with aqueous solutions of colchicine and DMS had some stimulating influence on their germination. On the contrary, the germination of the Scots pine seeds and the seeds collected from the Scots pine “WB” decreased with increasing the DMS concentration in the solution. Among the offspring obtained using chemical mutagens, specimens were identified with changes in the growth strength towards dwarfism, the branching features of which manifested themselves in the form of apical dominance violation and shoot formation growth, with changes in the color of needles, including winter color changes of different-intensity color from green to yellow, bronze or bronze yellow. As a result of the research, 363 specimens with atypical traits were selected for further breeding work.



Forests ◽  
2020 ◽  
Vol 11 (10) ◽  
pp. 1086
Author(s):  
Karolina Sobierajska ◽  
Witold Wachowiak ◽  
Julia Zaborowska ◽  
Bartosz Łabiszak ◽  
Błażej Wójkiewicz ◽  
...  

Scots pine (Pinus sylvestris L.) and the taxa from the P. mugo complex can hybridize in the contact zones and produce fertile hybrids. A unique example of an early Holocene relict population of P. sylvestris and P. uliginosa (a taxon from the P. mugo complex) growing on the tops of Jurassic sandstone rocks is located in Błędne Skały (Sudetes). Phenotypically, there are trees resembling P. sylvestris, P. uliginosa and intermediate forms between them. We expected that some of P. sylvestris and/or P. uliginosa-like trees could be in fact cryptic hybrids resembling one of the parental phenotypes. To address this question, we examined randomly sampled individuals, using a set of plastid (cpDNA), nuclear (nDNA) and mitochondrial (mtDNA) markers as well as biometric characteristics of needles and cones. The results were compared to the same measurements of allopatric reference populations of the P. sylvestris and the P. mugo complex (Pinus mugo s.s, P. uncinata and P. uliginosa). We detected cpDNA barcodes of the P. mugo complex in most individuals with the P. sylvestris phenotype, while we did not detect cpDNA diagnostic of P. sylvestris within P. uliginosa-like trees. These results indicate the presence of cryptic hybrids of the P. sylvestris phenotype. We found only three typical P. sylvestris individuals that were clustered with the species reference populations based on needle and cone characteristics. Most trees showed intermediate characteristics between P. sylvestris and P. uliginosa-like trees, indicating intensive and probably long-lasting hybridization of the taxa at this area and subsequent gene erosion of parental species.



2020 ◽  
Vol 19 (2) ◽  
pp. 75-85
Author(s):  
Sławomir Świerczyński ◽  
Marcin Kolasiński ◽  
Aleksander Stachowiak ◽  
Magdalena Rybus-Zając

The experimental studies were conducted from 2015–2017 years in two cycles. The aim of the research was to determine a better grafting time and a choice of an appropriate rootstock for the propagation of two cultivars of mountain pine (Pinus mugo Turra). The influence of the grafted cultivar and rootstock used on the level of chloroplast pigments in the needles was also checked. The studies concerned two cultivars of mountain pine (Pinus mugo) ‘Grześ’ and ‘Zundert’, which were grafted on four different rootstocks: Pinus contorta (Dougl. ex Loud.), Pinus nigra (Arn.), Pinus sylvestris (L.) and Pinus mugo subsp. uncinata (Ramond Domin) in two dates: 20 January and 15 March. The highest percentage of graft success was obtained for the two cultivars on Pinus uncinata and Pinus contorta rootstocks. A later term of grafting procedure affected bigger effectiveness of grafting of the two studied cultivars, except for the graft success on Pinus sylvestris rootstock. The highest increments of side shoots and lengths of main stems for ‘Grześ’ cultivar were obtained on Pinus nigra, and for ‘Zundert’ the influence of the rootstock on the above mentioned parameters was not evident. No significant differences in the number of increments in the first and second year of studies were observed. The highest level of chlorophyll A and B was found in the needles of ‘Grześ’ cultivar, independently from the rootstock used. Among tested rootstocks, only Pinus nigra had a significant impact on a higher content of chlorophyll B in the needles of the studied cultivars of mountain pine trees.



1996 ◽  
Vol 97 (4) ◽  
pp. 764-771 ◽  
Author(s):  
Q. Wang ◽  
C. H. A. Little ◽  
T. Moritz ◽  
P C. Oden
Keyword(s):  






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