Diversity of methanogenic archaeal communities in Japanese paddy field ecosystem, estimated by denaturing gradient gel electrophoresis

2010 ◽  
Vol 46 (4) ◽  
pp. 343-353 ◽  
Author(s):  
Takeshi Watanabe ◽  
Makoto Kimura ◽  
Susumu Asakawa
Keyword(s):  
Paddy Field ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Gradient Gel Electrophoresis ◽  
Field Ecosystem ◽  
Archaeal Communities

Temporal distribution of the archaeal community in the Changjiang Estuary hypoxia area and the adjacent East China Sea as determined by denaturing gradient gel electrophoresis and multivariate analysis

2011 ◽  
Vol 57 (6) ◽  
pp. 504-513 ◽  
Author(s):  
Min Liu ◽  
Tian Xiao ◽  
Ying Wu ◽  
Feng Zhou ◽  
Wuchang Zhang
Keyword(s):  
East China Sea ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Archaeal Community ◽  
Changjiang Estuary ◽  
East China ◽  
The Changjiang Estuary ◽  
China Sea ◽  
Gradient Gel Electrophoresis ◽  
Archaeal Communities

The archaeal community and the effects of environmental factors on microbial community distribution were investigated at five sampling sites in the Changjiang Estuary hypoxia area and the adjacent East China Sea in June, August, and October 2006. Profiles of the archaeal communities were generated by denaturing gradient gel electrophoresis of 16S rRNA genes followed by DNA sequence analysis, and the results were analyzed by multivariate statistical analysis. Denaturing gradient gel electrophoresis band patterns were analyzed by cluster analysis to assess temporal changes in the genetic diversity of the archaeal communities. Most of the October samples grouped together separately from those of June and August. Analysis of DNA sequences revealed that the dominant archaeal groups in the Changjiang Estuary hypoxia area and the adjacent East China Sea were affiliated with Euryarchaeota (mainly marine group II) and Crenarchaeota. The effects of environmental factors on the archaeal community distribution were analyzed by the ordination technique of canonical correspondence analysis. Salinity had a significant effect on the archaeal community composition.

scholarly journals Archaeal communities in two disparate deteriorated ancient wall paintings: detection, identification and temporal monitoring by denaturing gradient gel electrophoresis

2001 ◽  
Vol 37 (1) ◽  
pp. 45-54 ◽  
Author(s):  
Guadalupe Piñar ◽  
Cesareo Saiz-Jimenez ◽  
Claudia Schabereiter-Gurtner ◽  
Maria Teresa Blanco-Varela ◽  
Werner Lubitz ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Wall Paintings ◽  
Gradient Gel Electrophoresis ◽  
Archaeal Communities

Phylogenetic study on a bacterial community in the floodwater of a Japanese paddy field estimated by sequencing 16S rDNA fragments after denaturing gradient gel electrophoresis

2005 ◽  
Vol 42 (4) ◽  
pp. 362-365 ◽  
Author(s):  
Taketoshi Shibagaki-Shimizu ◽  
Natsuko Nakayama ◽  
Yasunori Nakajima ◽  
Kazuo Matsuya ◽  
Makoto Kimura ◽  
...  
Keyword(s):  
Bacterial Community ◽  
16S Rdna ◽  
Paddy Field ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Phylogenetic Study ◽  
Gradient Gel Electrophoresis

Allelopathic and Medicinal plant. 26. Millettia speciosa

2020 ◽  
Vol 51 (2) ◽  
pp. 125-146
Author(s):  
Nasiruddin Nasiruddin ◽  
Yu Zhangxin ◽  
Ting Zhao Chen Guangying ◽  
Minghui Ji
Keyword(s):  
Community Structure ◽  
Medicinal Plant ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Wiener Index ◽  
Pseudomonas Spp ◽  
Evenness Index ◽  
Gradient Gel Electrophoresis ◽  
Rhizosphere Pseudomonas

We grew cucumber in pots in greenhouse for 9-successive cropping cycles and analyzed the rhizosphere Pseudomonas spp. community structure and abundance by PCR-denaturing gradient gel electrophoresis and quantitative PCR. Results showed that continuous monocropping changed the cucumber rhizosphere Pseudomonas spp. community. The number of DGGE bands, Shannon-Wiener index and Evenness index decreased during the 3rd cropping and thereafter, increased up to the 7th cropping, however, however, afterwards they decreased again. The abundance of Pseudomonas spp. increased up to the 5th successive cropping and then decreased gradually. These findings indicated that the structure and abundance of Pseudomonas spp. community changed with long-term cucumber monocropping, which might be linked to soil sickness caused by its continuous monocropping.

Sign in / Sign up

Export Citation Format

Share Document