Localisation of bullous pemphigoid antigen 180 (BP180) in cultured human keratinocytes: functionally relevant modification by calcium

2006 ◽  
Vol 298 (6) ◽  
pp. 283-290 ◽  
Author(s):  
Enno Schmidt ◽  
Barbara Wehr ◽  
Katharina Wolf ◽  
Cassian Sitaru ◽  
Eva -B. Bröcker ◽  
...  
Keyword(s):  
Bullous Pemphigoid ◽  
Human Keratinocytes ◽  
Bullous Pemphigoid Antigen

scholarly journals Localization of Bullous Pemphigoid Antigen (BPA) in Isolated Human Keratinocytes

1985 ◽  
Vol 85 (3) ◽  
pp. 187-190 ◽  
Author(s):  
Marcelle Regnier ◽  
Pierre Vaigot ◽  
Serge Michel ◽  
Michel Prunieras
Keyword(s):  
Bullous Pemphigoid ◽  
Human Keratinocytes ◽  
Bullous Pemphigoid Antigen

scholarly journals Distinct functions for integrins alpha 3 beta 1 in focal adhesions and alpha 6 beta 4/bullous pemphigoid antigen in a new stable anchoring contact (SAC) of keratinocytes: relation to hemidesmosomes.

1990 ◽  
Vol 111 (6) ◽  
pp. 3141-3154 ◽  
Author(s):  
W G Carter ◽  
P Kaur ◽  
S G Gil ◽  
P J Gahr ◽  
E A Wayner
Keyword(s):  
Bullous Pemphigoid ◽  
Dominant Role ◽  
Focal Adhesions ◽  
Human Keratinocytes ◽  
Isolation Procedure ◽  
Basement Membrane Zone ◽  
Basal Cells ◽  
Bullous Pemphigoid Antigen ◽  
Insoluble Complex ◽  
Affinity Isolation

Basal cells of stratified epidermis are anchored to the basement membrane zone (BMZ) of skin via hemidesmosomes. We previously identified integrin alpha 3 beta 1, in focal adhesions (FAs), of cultured human keratinocytes (HFKs) as a mediator of HFK adhesion to secreted BMZ-like extracellular matrix (ECM; Carter, W.G., E.A. Wayner, T.S. Bouchard, and P. Kaur. 1990. J. Cell Biol. 110: 1387-1404). Here, we have examined the relation of integrins alpha 6 beta 4 and alpha 3 beta 1, to bullous pemphigoid antigen (BPA), a component of hemidesmosomes. We conclude that alpha 6 beta 4 in HFKs localizes in a new stable anchoring contact (SAC) that cooperates with alpha 3 beta 1-FAs to mediate adhesion to ECM, based on the following. (a) Comparison of secreted ECM, with exogenous laminin, fibronectin and collagen identified ECM as the preferred ligand for HFK adhesion and spreading and for formation of both alpha 6 beta 4-SACs and alpha 3 beta 1-FAs. (b) Inhibition of HFK adhesion with combined anti-alpha 3 beta 1 (P1B5) and anti-alpha 6 beta 4 (GoH3) antibodies indicated that both receptors were functional in adhesion to ECM while alpha 3 beta 1 played a dominant role in spreading. (c) alpha 6 beta 4 colocalized with BPA in SACs that were proximal to but excluded from FAs. Both alpha 6 beta 4-SACs and alpha 3 beta 1-FAs were in contact with the adhesion surface as indicated by antibody exclusion and interference reflection microscopy. (d) In contrast to alpha 3 beta 1-FAs, alpha 6 beta 4-SACs were present only in nonmotile cells, not associated with stress fibers, and were relatively stable to detergents and urea, suggesting a nonmotile, or anchoring function for SACs and motility functions for alpha 3 beta 1-FAs. (e) alpha 6 beta 4 formed a detergent-insoluble complex with exogenous ECM in an affinity isolation procedure, confirming the ability of an unidentified ECM ligand to interact with alpha 6 beta 4. (f) We suggest that alpha 6 beta 4/BPA-SACs in culture restrict migration of HFKs on ECM while alpha 3 beta 1-FAs form dynamic adhesions in spreading and migrating cells. alpha 6 beta 4/BPA-SACs in culture bear functional and compositional similarities to hemidesmosomes in skin.

Enhancement of bullous pemphigoid antigen gene expression by γ-interferon in cultured human keratinocytes

1990 ◽  
Vol 1 (5) ◽  
pp. 383
Author(s):  
Yasuyuki Sugita ◽  
Tetsuo Nagatani ◽  
Zenro Ikezawa ◽  
Kazuo Nomura ◽  
Hiroshi Nakajima
Keyword(s):  
Gene Expression ◽  
Bullous Pemphigoid ◽  
Human Keratinocytes ◽  
Antigen Gene ◽  
Bullous Pemphigoid Antigen ◽  
Γ Interferon

scholarly journals Comparison of molecularly cloned bullous pemphigoid antigen to desmoplakin I confirms that they define a new family of cell adhesion junction plaque proteins

1991 ◽  
Vol 266 (19) ◽  
pp. 12555-12559
Author(s):  
T. Tanaka ◽  
D.A. Parry ◽  
V. Klaus-Kovtun ◽  
P.M. Steinert ◽  
J.R. Stanley
Keyword(s):  
Cell Adhesion ◽  
Bullous Pemphigoid ◽  
Bullous Pemphigoid Antigen ◽  
New Family

Activation of bullous pemphigoid antigen gene in mouse ear epidermis by ultraviolet radiation

1998 ◽  
Vol 16 ◽  
pp. S228
Author(s):  
Ken-ichi Kayashima ◽  
Takehiko Koji ◽  
Masayuki Nozawa ◽  
Tomomichi Ono ◽  
Paul K. Nakane
Keyword(s):  
Ultraviolet Radiation ◽  
Bullous Pemphigoid ◽  
Antigen Gene ◽  
Bullous Pemphigoid Antigen ◽  
Mouse Ear
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