Development of a short-term human full-thickness skin organ culture model in vitro under serum-free conditions

Abstract Background The repair of large-scale full-thickness skin defects represents a challenging obstacle in skin tissue engineering. To address the most important problem in skin defect repair, namely insufficient blood supply, this study aimed to find a method that could promote the formation of vascularized skin tissue. Method The phenotypes of ASCs and EPCs were identified respectively, and ASCs/EPCs were co-cultured in vitro to detect the expression of dermal and angiogenic genes. Furthermore, the co-culture system combined with dermal extracellular matrix hydrogel was used to repair the full-scale skin defects in rats.Result The co-culture of ASCs/EPCs could increase skin and angiogenesis-related gene expression in vitro. The results of in vivo animal experiments demonstrated that the ASCs/EPCs group could significantly accelerate the repair of skin defects by promoting the regeneration of vascularized skin.Conclusion It is feasible to replace traditional single seed cells with ASC/EPC co-culture system for vascularized skin regeneration. This system could ultimately enable clinicians to better repair the full-thickness skin defects and avoid donor site morbidity.

Download Full-text

Comparative analysis of the IFNG, IL17A, and IL22-regulated transcriptomes in reconstituted human epidermis (RHE), an in-vitro model of full-thickness skin

SPP Datasets ◽

10.1621/sntvfe7xef ◽

2020 ◽

Author(s):

M Suárez-Fariñas

Keyword(s):

Comparative Analysis ◽

In Vitro Model ◽

Human Epidermis ◽

Full Thickness ◽

Full Thickness Skin ◽

Thickness Skin ◽

Vitro Model

Download Full-text

The Australian Mohs Database: Short-Term Recipient-Site Complications in Full-Thickness Skin Grafts

Dermatologic Surgery ◽

10.1111/j.1524-4725.2006.32307.x ◽

2006 ◽

Vol 32 (11) ◽

pp. 1364-1368 ◽

Cited By ~ 7

Author(s):

IGAL LEIBOVITCH ◽

SHYAMALA C. HUILGOL ◽

SHAWN RICHARDS ◽

ROBERT PAVER ◽

DINESH SELVA

Keyword(s):

Skin Grafts ◽

Full Thickness ◽

Short Term ◽

Recipient Site ◽

Full Thickness Skin ◽

Thickness Skin

Download Full-text

Derivation of avian dermal LD50 values for dermal exposure models using in vitro percutaneous absorption of [14C]-atrazine through rat, mallard, and northern bobwhite full thickness skin

The Science of The Total Environment ◽

10.1016/j.scitotenv.2018.02.206 ◽

2018 ◽

Vol 630 ◽

pp. 517-525 ◽

Cited By ~ 2

Author(s):

Jonathan D. Maul ◽

Craig Blackstock ◽

Richard A. Brain

Keyword(s):

Percutaneous Absorption ◽

Dermal Exposure ◽

Northern Bobwhite ◽

Full Thickness ◽

Exposure Models ◽

Full Thickness Skin ◽

Thickness Skin ◽

In Vitro Percutaneous Absorption

Download Full-text

A First in Human Trial Implanting Microalgae Shows Safety of Photosynthetic Therapy for the Effective Treatment of Full Thickness Skin Wounds

Frontiers in Medicine ◽

10.3389/fmed.2021.772324 ◽

2021 ◽

Vol 8 ◽

Author(s):

Miguel Luis Obaíd ◽

Juan Pablo Camacho ◽

Marianne Brenet ◽

Rocío Corrales-Orovio ◽

Felipe Carvajal ◽

...

Keyword(s):

Clinical Trial ◽

Phase 1 ◽

Skin Wounds ◽

Full Thickness ◽

Clinical Trial Registration ◽

Full Thickness Skin ◽

Thickness Skin ◽

Photosynthetic Cells

Insufficient oxygen supply represents a relevant issue in several fields of human physiology and medicine. It has been suggested that the implantation of photosynthetic cells can provide oxygen to tissues in the absence of a vascular supply. This approach has been demonstrated to be successful in several in vitro and in vivo models; however, no data is available about their safety in human patients. Here, an early phase-1 clinical trial (ClinicalTrials.gov identifier: NCT03960164, https://clinicaltrials.gov/ct2/show/NCT03960164) is presented to evaluate the safety and feasibility of implanting photosynthetic scaffolds for dermal regeneration in eight patients with full-thickness skin wounds. Overall, this trial shows that the presence of the photosynthetic microalgae Chlamydomonas reinhardtii in the implanted scaffolds did not trigger any deleterious local or systemic immune responses in a 90 days follow-up, allowing full tissue regeneration in humans. The results presented here represent the first attempt to treat patients with photosynthetic cells, supporting the translation of photosynthetic therapies into clinics.Clinical Trial Registration:www.clinicaltrials.gov/ct2/show/NCT03960164, identifier: NCT03960164.

Download Full-text