scholarly journals The In Vitro Hydrolysis of Diisopropyl Fluorophosphate During Penetration Through Human Full-Thickness Skin and Isolated Epidermis

1985 ◽  
Vol 85 (4) ◽  
pp. 335-339 ◽  
Author(s):  
Marie Lodén
Keyword(s):  
Full Thickness ◽  
Diisopropyl Fluorophosphate ◽  
Full Thickness Skin ◽  
In Vitro Hydrolysis ◽  
Thickness Skin ◽  
Hydrolysis Of

scholarly journals The Co-Culture of ASCs and EPCs Promotes Wound Healing by Promoting Angiogenesis in Full Thickness Skin Defects

2020 ◽  
Author(s):  
Shuang Lin ◽  
Xiaoning He ◽  
Yuanjia He
Keyword(s):  
Culture System ◽  
Large Scale ◽  
Donor Site ◽  
Skin Tissue ◽  
Skin Defect ◽  
Full Thickness ◽  
Skin Defects ◽  
Full Thickness Skin ◽  
Thickness Skin

Abstract Background The repair of large-scale full-thickness skin defects represents a challenging obstacle in skin tissue engineering. To address the most important problem in skin defect repair, namely insufficient blood supply, this study aimed to find a method that could promote the formation of vascularized skin tissue. Method The phenotypes of ASCs and EPCs were identified respectively, and ASCs/EPCs were co-cultured in vitro to detect the expression of dermal and angiogenic genes. Furthermore, the co-culture system combined with dermal extracellular matrix hydrogel was used to repair the full-scale skin defects in rats.Result The co-culture of ASCs/EPCs could increase skin and angiogenesis-related gene expression in vitro. The results of in vivo animal experiments demonstrated that the ASCs/EPCs group could significantly accelerate the repair of skin defects by promoting the regeneration of vascularized skin.Conclusion It is feasible to replace traditional single seed cells with ASC/EPC co-culture system for vascularized skin regeneration. This system could ultimately enable clinicians to better repair the full-thickness skin defects and avoid donor site morbidity.

scholarly journals A First in Human Trial Implanting Microalgae Shows Safety of Photosynthetic Therapy for the Effective Treatment of Full Thickness Skin Wounds

2021 ◽  
Vol 8 ◽  
Author(s):  
Miguel Luis Obaíd ◽  
Juan Pablo Camacho ◽  
Marianne Brenet ◽  
Rocío Corrales-Orovio ◽  
Felipe Carvajal ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Phase 1 ◽  
Skin Wounds ◽  
Full Thickness ◽  
Clinical Trial Registration ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Photosynthetic Cells

Insufficient oxygen supply represents a relevant issue in several fields of human physiology and medicine. It has been suggested that the implantation of photosynthetic cells can provide oxygen to tissues in the absence of a vascular supply. This approach has been demonstrated to be successful in several in vitro and in vivo models; however, no data is available about their safety in human patients. Here, an early phase-1 clinical trial (ClinicalTrials.gov identifier: NCT03960164, https://clinicaltrials.gov/ct2/show/NCT03960164) is presented to evaluate the safety and feasibility of implanting photosynthetic scaffolds for dermal regeneration in eight patients with full-thickness skin wounds. Overall, this trial shows that the presence of the photosynthetic microalgae Chlamydomonas reinhardtii in the implanted scaffolds did not trigger any deleterious local or systemic immune responses in a 90 days follow-up, allowing full tissue regeneration in humans. The results presented here represent the first attempt to treat patients with photosynthetic cells, supporting the translation of photosynthetic therapies into clinics.Clinical Trial Registration:www.clinicaltrials.gov/ct2/show/NCT03960164, identifier: NCT03960164.

scholarly journals Development of an Aged Full-Thickness Skin Model Using Flexible Skin-on-a-Chip Subjected to Mechanical Stimulus Reflecting the Circadian Rhythm

2021 ◽  
Vol 22 (23) ◽  
pp. 12788
Author(s):  
Subin Jeong ◽  
Jisue Kim ◽  
Hye Mi Jeon ◽  
Kyunghee Kim ◽  
Gun Yong Sung
Keyword(s):  
Circadian Rhythm ◽  
Accelerated Aging ◽  
Skin Equivalent ◽  
Full Thickness ◽  
Skin Model ◽  
Flexible Skin ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Aging Skin

The skin is subject to both intrinsic aging caused by metabolic processes in the body and extrinsic aging caused by exposure to environmental factors. Intrinsic aging is an important obstacle to in vitro experimentation as its long-term progression is difficult to replicate. Here, we accelerated aging of a full-thickness skin equivalent by applying periodic mechanical stimulation, replicating the circadian rhythm for 28 days. This aging skin model was developed by culturing a full-thickness, three-dimensional skin equivalent with human fibroblasts and keratinocytes to produce flexible skin-on-a-chip. Accelerated aging associated with periodic compressive stress was evidenced by reductions in the epidermal layer thickness, contraction rate, and secretion of Myb. Increases in β-galactosidase gene expression and secretion of reactive oxygen species and transforming growth factor-β1 were also observed. This in vitro aging skin model is expected to greatly accelerate drug development for skin diseases and cosmetics that cannot be tested on animals.

scholarly journals Co-culture of ASCs/EPCs and dermal extracellular matrix hydrogel enhances the repair of full-thickness skin wound by promoting angiogenesis

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Shuang Lin ◽  
Xiaoning He ◽  
Yuanjia He
Keyword(s):  
Extracellular Matrix ◽  
Culture System ◽  
Large Scale ◽  
Skin Tissue ◽  
Skin Defect ◽  
Full Thickness ◽  
Skin Defects ◽  
Full Thickness Skin ◽  
Thickness Skin

Abstract Background The repair of large-scale full-thickness skin defects represents a challenging obstacle in skin tissue engineering. To address the most important problem in skin defect repair, namely insufficient blood supply, this study aimed to find a method that could promote the formation of vascularized skin tissue. Method The phenotypes of ASCs and EPCs were identified respectively, and ASCs/EPCs were co-cultured in vitro to detect the expression of dermal and angiogenic genes. Furthermore, the co-culture system combined with dermal extracellular matrix hydrogel was used to repair the full-scale skin defects in rats. Result The co-culture of ASCs/EPCs could increase skin- and angiogenesis-related gene expression in vitro. The results of in vivo animal experiments demonstrated that the ASCs/EPCs group could significantly accelerate the repair of skin defects by promoting the regeneration of vascularized skin. Conclusion It is feasible to replace traditional single-seed cells with the ASC/EPC co-culture system for vascularized skin regeneration. This system could ultimately enable clinicians to better repair the full-thickness skin defects and avoid donor site morbidity.

scholarly journals In vitro 3D Full-Thickness Skin-Equivalent Tissue Model Using Silk and Collagen Biomaterials

2012 ◽  
Vol 12 (12) ◽  
pp. 1627-1636 ◽  
Author(s):  
Evangelia Bellas ◽  
Miri Seiberg ◽  
Jonathan Garlick ◽  
David L. Kaplan
Keyword(s):  
Skin Equivalent ◽  
Full Thickness ◽  
Tissue Model ◽  
Full Thickness Skin ◽  
Thickness Skin

scholarly journals Heparinized silk fibroin hydrogels loading FGF1 promote the wound healing in rats with full-thickness skin excision

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Sirong He ◽  
Dan Shi ◽  
Zhigang Han ◽  
Zhaoming Dong ◽  
Yajun Xie ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silk Fibroin ◽  
Wound Repair ◽  
Histological Evaluation ◽  
Full Thickness ◽  
Acidic Fibroblast Growth Factor ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Skin Excision

Abstract Background Silk fibroin hydrogel, derived from Bombyx mori cocoons, has been shown to have potential effects on wound healing due to its excellent biocompatibility and less immunogenic and biodegradable properties. Many studies suggest silk fibroin as a promising material of wound dressing and it can support the adhesion and proliferation of a variety of human cells in vitro. However, lack of translational evidence has hampered its clinical applications for skin repair. Herein, a heparin-immobilized fibroin hydrogel was fabricated to deliver FGF1 (human acidic fibroblast growth factor 1) on top of wound in rats with full-thickness skin excision by performing comprehensive preclinical studies to fully evaluate its safety and effectiveness. The wound-healing efficiency of developed fibroin hydrogels was evaluated in full-thickness wound model of rats, compared with the chitosan used clinically. Results The water absorption, swelling ratio, accumulative FGF1 releasing rate and biodegradation ratio of fabricated hydrogels were measured. The regenerated fibroin hydrogels with good water uptake properties rapidly swelled to a 17.3-fold maximum swelling behavior over 12 h and a total amount of 40.48 ± 1.28% hydrogels was lost within 15 days. Furthermore, accumulative releasing data suggested that heparinized hydrogels possessed effective release behavior of FGF1. Then full-thickness skin excision was created in rats and left untreated or covered with heparinized fibroin hydrogels-immobilized recombinant human FGF1. The histological evaluation using hematoxylin and eosin (HE) and Masson’s trichrome (MT) staining was performed to observe the dermic formation and collagen deposition on the wound-healing site. To evaluate the wound-healing mechanisms induced by fibroin hydrogel treatment, wound-healing scratch and cell proliferation assay were performed. it was found that both fibroin hydrogels and FGF1 can facilitate the migration of fibroblast L929 cells proliferation and migration. Conclusion This study provides systematic preclinical evidence that the silk fibroin promotes wound healing as a wound-healing dressing, thereby establishing a foundation toward its further application for new treatment options of wound repair and regeneration.

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