scholarly journals Circulating Endothelial Cells and Endothelial Progenitor Cells in Obstructive Sleep Apnea

Lung ◽  
2008 ◽  
Vol 186 (3) ◽  
pp. 145-150 ◽  
Author(s):  
Kevin Martin ◽  
Michael Stanchina ◽  
Nicola Kouttab ◽  
Elizabeth O. Harrington ◽  
Sharon Rounds
Keyword(s):  
Endothelial Cells ◽  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Circulating Endothelial Cells ◽  
Endothelial Progenitor ◽  
Obstructive Sleep

scholarly journals The Association of Nephroblastoma Overexpressed (NOV) and Endothelial Progenitor Cells with Oxidative Stress in Obstructive Sleep Apnea

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Eddie W. Fakhouri ◽  
Jeremy A. Weingarten ◽  
Shailendra P. Singh ◽  
Purvi Shah ◽  
Stephen J. Peterson
Keyword(s):  
Oxidative Stress ◽  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Subsequent Development ◽  
Circulating Endothelial Cells ◽  
Endothelial Progenitor ◽  
Obstructive Sleep ◽  
Age And Sex

Objective. Obstructive sleep apnea (OSA) is a sleep disorder characterized by intermittent hypoxia, chronic inflammation, and oxidative stress and is associated with cardiometabolic disease. Several biological substrates have been associated with OSA such as nephroblastoma overexpressed (NOV), endothelial progenitor cells (EPC), and circulating endothelial cells (CEC). Few studies have looked at the association of NOV with OSA while the EPC/CEC relationships with OSA are unclear. In this study, we hypothesize that (1) NOV is associated with the severity of OSA independent of BMI, identifying a protein that may play a role in the biogenesis of OSA complications, and (2) EPCs and CECs are also associated with the severity of OSA and are biomarkers of endothelial dysfunction in OSA. Methods. 61 subjects underwent overnight polysomnography (PSG), clinical evaluation, and blood analysis for NOV, EPC, CEC, interleukin 6 (IL-6), and other potential biomarkers. Results. NOV and EPCs were independently associated with the oxygen desaturation index (ODI) after adjusting for potential confounders including body mass index (BMI), age, and sex (NOV p = 0.032 ; EPC p = 0.001 ). EPC was also independently associated with AHI after adjusting for BMI, age, and sex ( p = 0.017 ). IL-6 was independently associated with AHI, but not with ODI. Conclusion. NOV and EPC levels correlate with the degree of OSA independent of BMI, indicating that these biomarkers could potentially further elucidate the relationship between OSA patients and their risk of the subsequent development of cardiovascular disease.

scholarly journals Altered profile of circulating endothelial progenitor cells in obstructive sleep apnea

2012 ◽  
Vol 17 (3) ◽  
pp. 937-942 ◽  
Author(s):  
Macy Mei-Sze Lui ◽  
Hung-Fat Tse ◽  
Judith Choi-Wo Mak ◽  
Jamie Chung-Mei Lam ◽  
David Chi-Leung Lam ◽  
...  
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Endothelial Progenitor ◽  
Circulating Endothelial Progenitor Cells ◽  
Obstructive Sleep

Presence of endothelial progenitor cells, distinct from mature endothelial cells, within human CD146+ blood cells

2005 ◽  
Vol 94 (12) ◽  
pp. 1270-1279 ◽  
Author(s):  
Bruno Delorme ◽  
Agnès Basire ◽  
Carla Gentile ◽  
Florence Sabatier ◽  
Frédéric Monsonis ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Network Formation ◽  
Mononuclear Cells ◽  
Circulating Endothelial Cells ◽  
Endothelial Progenitor ◽  
Color Flow ◽  
Immunodeficient Mice ◽  
Circulating Cells

SummaryCD146 is an adhesion molecule present on endothelial cells throughout the vascular tree. CD146 is also expressed by circulating endothelial cells (CECs) widely considered to be mature endothelial cells detached from injured vessels. The discovery of circulating endothelial progenitor cells (EPCs) originating from bone marrow prompted us to investigate whether CD146 circulating cells could also contains EPCs. We tested this hypothesis using an approach combining elimination of CECs by an adhesion step, followed by immunomagnetic sorting of remaining CD146+ cells from the non adherent fraction of cord blood mononuclear cells. When cultured under endothelial-promoting conditions, these cells differentiated as late outgrowth endothelial colonies: they grew as a cobblestone monolayer, were uniformly positive for endothelial markers and did not express leukocyte antigens. They highly proliferated and were expanded in long-term culture without alterations of their phenotypic and functional properties (DiI-ac-LDL uptake, wound repair, capillary-like network formation, and TNFα response). Moreover, these cells colonized a Matrigel plug in immunodeficient mice (NOD/SCID). Finally, using 4-color flow cytometry analysis of purified CD34+ cells, we clearly discriminated, CD146+ EPCs (CD146+ CD34+ CD45+ CD133+ or CD117+), and CD146+ CECs (CD146+ CD34+, CD45− CD133− or CD117−), both in cord and adult peripheral blood. The relative proportions of the two CD146+ subsets varied in patients with myocardial infarction as compared to healthy subjects. Our study establishes that, beside CECs, CD146+ circulating cells contain a subpopulation of EPCs with potential use in proangiogenic therapy. In addition, the dual measurement of CD146+ CECs and CD146+ EPCs offers a promising tool for monitoring vascular injury/regeneration processes in clinical situations.

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