Corneal biopsy for diagnosis of recalcitrant microbial keratitis

2018 ◽  
Vol 256 (8) ◽  
pp. 1527-1533 ◽  
Author(s):  
Dana Robaei ◽  
U-Teng Chan ◽  
Pauline Khoo ◽  
Svetlana Cherepanoff ◽  
Yi-Chiao Li ◽  
...  
Keyword(s):  
Microbial Keratitis ◽  
Corneal Biopsy

Corneal biopsy in microbial keratitis

1991 ◽  
Vol 19 (3) ◽  
pp. 193-196 ◽  
Author(s):  
G. Whitehouse ◽  
K. Reid ◽  
B. Hudson ◽  
V.A. Lennox ◽  
M.A. Lawless
Keyword(s):  
Microbial Keratitis ◽  
Corneal Biopsy

Corneal biopsy in the management of progressive microbial keratitis

2000 ◽  
Vol 129 (5) ◽  
pp. 571-576 ◽  
Author(s):  
George Alexandrakis ◽  
Robert Haimovici ◽  
Darlene Miller ◽  
Eduardo C Alfonso
Keyword(s):  
Microbial Keratitis ◽  
Corneal Biopsy

scholarly journals Organism Isolation in Corneal Ulcer- Utility of Different Techniques

2012 ◽  
Vol 52 (185) ◽  
Author(s):  
M Thapa ◽  
A K Sharma ◽  
D N Shah ◽  
S KC ◽  
S Shrestha
Keyword(s):  
Corneal Ulcer ◽  
Descriptive Study ◽  
Microbial Keratitis ◽  
Cross Sectional ◽  
Corneal Ulcers ◽  
Isolation Techniques ◽  
Corneal Scraping ◽  
Microbial Agents ◽  
Culture Negative ◽  
Corneal Biopsy

Introduction: Various techniques are available for isolation of microorganism in cases of microbial keratitis including conventional methods of scraping, re-scraping inoculation in different media and corneal biopsy. Methods: This was a cross sectional descriptive study conducted from February 2002 to July 2003. A total of 161 eyes of corneal ulcers were evaluated with scraping inoculation. Re-scraping and corneal biopsy were performed in those cases which did not grow any organism in the first scraping or which did not respond to initial treatment. Results: Microorganisms were isolated in 116 patients (72.04%) after first scraping. S pneumoniae was seen in 50(42%), Fusarium in 21(18%) and Aspergillus in 16(14%) cases. Re-scraping was done in 22 culture negative and non improving cases of which 14 (63.63%) showed isolation of organisms. After re-scraping, Fusarium and Aspergillus were isolated in four patients each (18% each) followed by S. pneumoniae in 3 (14%), S. aureus in 2 (9%), and Penicillium in 1(5%) case.Corneal biopsy was done in 10 cases with isolation of microbial agents in 7 (70%) with Aspergillus and S. aureus in three each, and S. epidermidis in 1(10%). Conclusions: Apart from conventional scraping, other isolation techniques should be considered in the treatment of non-responding and culture negative cases of corneal ulcers. Compared to bacteria, fungi are difficult to isolate in the first scraping and requires more frequent repeat scraping and corneal biopsy. Keywords: Corneal biopsy, corneal scraping, corneal ulcer, microbial keratitis, organismal isolation.

Diagnosis of Microbial Keratitis Causatives by Polymerase Chain Reaction

2013 ◽  
Vol 16 (3) ◽  
pp. 183-190
Author(s):  
Rasha S. Ameen ◽  
◽  
Faiz I. Al-Shakarchi ◽  
Abdulwahid B. A. Al-Shaibani ◽  
◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Microbial Keratitis ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals A ten-year report of microbial keratitis in pediatric population under five years in a tertiary eye center

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Mohammad Soleimani ◽  
Seyed Ali Tabatabaei ◽  
S. Saeed Mohammadi ◽  
Niloufar Valipour ◽  
Arash Mirzaei
Keyword(s):  
Pediatric Population ◽  
Cross Sectional Study ◽  
Eye Drops ◽  
Microbial Keratitis ◽  
Cyanoacrylate Glue ◽  
Cross Sectional ◽  
Under Five ◽  
Fluoroquinolone Antibiotics ◽  
Empiric Antibiotic ◽  
Corneal Scraping

Abstract Purpose To report characteristics of microbial keratitis in pediatric patients under five years. Methods Patients with infectious keratitis under the age of 5 years were included in this retrospective cross-sectional study for ten years. All patients were admitted and corneal scraping was performed in 81 children. Fortified empiric antibiotic eye drops including cefazolin (50 mg/cc) and amikacin (20 mg/cc) were started and the antibiotic regimen was continued or changed according to culture results. In the case of fungal keratitis, topical voriconazole (10 mg/cc) or natamycin (50 mg/cc) and topical chloramphenicol (5 mg/cc) were started. A tectonic procedure was done when corneal thinning or perforation was present. Results Ninety-Three Patients between 1 to 60 months with a mean age of 33 ± 18 months old with corneal ulcer were included in the study. The most common risk factor was trauma (40.9%) followed by contact lens use (8.6%). Cultures were negative for microbial growth in 28 (30.1%) patients. The most common pathogens were S. epidermidis (10.8%) and P. aeruginosa (10.8%). Fluoroquinolone antibiotics (ciprofloxacin; 93.8% sensitivity) were the most potent antibiotic against bacterial pathogens. Forty-one patients underwent tectonic procedures, which the most common ones were cyanoacrylate glue 18.3% followed by keratoplasty 16.1%. Conclusion This study emphasizes the role of trauma as the primary cause and S. epidermidis as the most frequent microorganism in pediatric keratitis; according to antibiogram results and poor cooperation of patients under five years, monotherapy with fluoroquinolones could be a good regimen in small non-central lesions without thinning.

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