Use of dual section mRNA in situ hybridisation/immunohistochemistry to clarify gene expression patterns during the early stages of nephron development in the embryo and in the mature nephron of the adult mouse kidney

2008 ◽  
Vol 130 (5) ◽  
pp. 927-942 ◽  
Author(s):  
Kylie Georgas ◽  
Bree Rumballe ◽  
Lorine Wilkinson ◽  
Han Sheng Chiu ◽  
Emmanuelle Lesieur ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adult Mouse ◽  
Expression Patterns ◽  
In Situ Hybridisation ◽  
Mouse Kidney ◽  
Gene Expression Patterns ◽  
Early Stages ◽  
Nephron Development ◽  
Mrna In Situ Hybridisation

scholarly journals RNA In Situ Hybridization for Detecting Gene Expression Patterns in the Abdomens and Wings of Drosophila Species

2021 ◽  
Vol 4 (1) ◽  
pp. 20
Author(s):  
Mujeeb Shittu ◽  
Tessa Steenwinkel ◽  
William Dion ◽  
Nathan Ostlund ◽  
Komal Raja ◽  
...  
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Expression Patterns ◽  
Drosophila Species ◽  
Gene Expression Patterns ◽  
Probe Design ◽  
Tissue Preparation ◽  
Design And Synthesis ◽  
Rna In Situ Hybridization

RNA in situ hybridization (ISH) is used to visualize spatio-temporal gene expression patterns with broad applications in biology and biomedicine. Here we provide a protocol for mRNA ISH in developing pupal wings and abdomens for model and non-model Drosophila species. We describe best practices in pupal staging, tissue preparation, probe design and synthesis, imaging of gene expression patterns, and image-editing techniques. This protocol has been successfully used to investigate the roles of genes underlying the evolution of novel color patterns in non-model Drosophila species.

scholarly journals Differential gene expression patterns of the developing and adult mouse cornea compared to the lens and tendon

2008 ◽  
Vol 87 (3) ◽  
pp. 214-225 ◽  
Author(s):  
Feng Wu ◽  
Seakwoo Lee ◽  
Michael Schumacher ◽  
Albert Jun ◽  
Shukti Chakravarti
Keyword(s):  
Gene Expression ◽  
Differential Gene Expression ◽  
Adult Mouse ◽  
Expression Patterns ◽  
Gene Expression Patterns ◽  
Differential Gene
Sign in / Sign up

Export Citation Format

Share Document