Porcine ovarian cortex-derived putative stem cells can differentiate into endothelial cells in vitro

AbstractEndothelial cells (ECs), the primary component of the vasculature, play a crucial role in neovascularization. However, the number of endogenous ECs is inadequate for both experimental purposes and clinical applications. Porcine ovarian putative stem cells (poPSCs), although not pluripotent, are characterized by great plasticity. Therefore, this study aimed to investigate whether poPSCs have the potential to differentiate into cells of endothelial lineage. poPSCs were immunomagnetically isolated from postnatal pig ovaries based on the presence of SSEA-4 protein. Expression of mesenchymal stem cells (MSCs) markers after pre-culture, both at the level of mRNA: ITGB1, THY, and ENG and corresponding protein: CD29, CD90, and CD105 were significantly higher compared to the control ovarian cortex cells. To differentiate poPSCs into ECs, inducing medium containing vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), insulin-like growth factor (IGF), epidermal growth factor (EGF), ascorbic acid, and heparin was applied. After 14 days, poPSC differentiation into ECs was confirmed by immunofluorescence staining for vascular endothelial cadherin (VECad) and vascular endothelial growth factor receptor-2 (VEGFR-2). Semi-quantitative WB analysis of these proteins confirmed their high abundance. Additionally, qRT-PCR showed that mRNA expression of corresponding marker genes: CDH5, KDR was significantly higher compared with undifferentiated poPSCs. Finally, EC functional status was confirmed by the migration test that revealed that they were capable of positive chemotaxis, while tube formation assay demonstrated their ability to develop capillary networks. In conclusion, our results provided evidence that poPSCs may constitute the MSC population in the ovary and confirmed that they might be a potential source of ECs for tissue engineering.

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Human antigen R promotes angiogenesis of endothelial cells cultured with adipose stem cells derived exosomes via overexpression of vascular endothelial growth factor in vitro

Adipocyte ◽

10.1080/21623945.2021.1982577 ◽

2021 ◽

Vol 10 (1) ◽

pp. 475-482

Author(s):

Guo Li ◽

Youbai Chen ◽

Yudi Han ◽

Tian Ma ◽

Yan Han

Keyword(s):

Stem Cells ◽

Vascular Endothelial Growth Factor ◽

Endothelial Cells ◽

Growth Factor ◽

Adipose Stem Cells ◽

Vascular Endothelial ◽

Human Antigen R ◽

Endothelial Growth Factor ◽

Cells Cultured

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Heparin‐modified alginate microspheres enhance neovessel formation in hiPSC ‐derived endothelial cells and heterocellular in vitro models by controlled release of vascular endothelial growth factor

Journal of Biomedical Materials Research Part A ◽

10.1002/jbm.a.37168 ◽

2021 ◽

Author(s):

Fabiola Munarin ◽

Carly Kabelac ◽

Kareen L.K. Coulombe

Keyword(s):

Vascular Endothelial Growth Factor ◽

Endothelial Cells ◽

Controlled Release ◽

Growth Factor ◽

In Vitro Models ◽

Vascular Endothelial ◽

Endothelial Growth Factor

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Vascular endothelial growth factor-stimulated cerebral microvascular endothelial cells mediate the recruitment of neural stem cells to the neurovascular niche

Brain Research ◽

10.1016/j.brainres.2009.02.065 ◽

2009 ◽

Vol 1268 ◽

pp. 24-37 ◽

Cited By ~ 59

Author(s):

Nils O. Schmidt ◽

Dennis Koeder ◽

Markus Messing ◽

Franz-Josef Mueller ◽

Karen S. Aboody ◽

...

Keyword(s):

Stem Cells ◽

Vascular Endothelial Growth Factor ◽

Endothelial Cells ◽

Growth Factor ◽

Neural Stem Cells ◽

Microvascular Endothelial Cells ◽

Vascular Endothelial ◽

Microvascular Endothelial ◽

Neurovascular Niche ◽

Endothelial Growth Factor

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Transgenic Overexpression of Vascular Endothelial Growth Factor-B Isoforms by Endothelial Cells Potentiates Postnatal Vessel Growth In Vivo and In Vitro

Circulation Research ◽

10.1161/01.res.0000182631.33638.77 ◽

2005 ◽

Vol 97 (6) ◽

Cited By ~ 38

Author(s):

Arne W. Mould ◽

Sonia A. Greco ◽

Marian M. Cahill ◽

Ian D. Tonks ◽

Daniela Bellomo ◽

...

Keyword(s):

Vascular Endothelial Growth Factor ◽

Endothelial Cells ◽

Growth Factor ◽

Vascular Endothelial ◽

Factor B ◽

Vessel Growth ◽

Endothelial Growth Factor

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Isolation and Characterisation of Lymphatic Endothelial Cells from Lung Tissues Affected by Lymphangioleiomyomatosis

10.21203/rs.3.rs-155950/v1 ◽

2021 ◽

Author(s):

Koichi Nishino ◽

Yasuhiro Yoshimatsu ◽

Tomoki Muramatsu ◽

Yasuhito Sekimoto ◽

Keiko Mitani ◽

...

Keyword(s):

Vascular Endothelial Growth Factor ◽

Endothelial Cells ◽

Growth Factor ◽

Normal Lung ◽

Vascular Endothelial ◽

Lymphatic Endothelial Cells ◽

And Migration ◽

Endothelial Growth Factor ◽

Proliferation And Migration

Abstract Lymphangioleiomyomatosis (LAM) is a rare pulmonary disease characterised by the proliferation of smooth muscle-like cells (LAM cells), and an abundance of lymphatic vessels in LAM lesions. Studies reported that vascular endothelial growth factor-D (VEGF-D) secreted by LAM cells contributes to LAM-associated lymphangiogenesis, however, the precise mechanisms of lymphangiogenesis and characteristics of lymphatic endothelial cells (LECs) in LAM lesions have not yet been elucidated. In this study, human primary-cultured LECs were obtained both from LAM-affected lung tissues (LAM-LECs) and normal lung tissues (control LECs) using fluorescence-activated cell sorting (FACS). We found that LAM-LECs had significantly higher ability of proliferation and migration compared to control LECs. VEGF-D significantly promoted migration of LECs but not proliferation of LECs in vitro. cDNA microarray and FACS analysis revealed the expression of vascular endothelial growth factor receptor (VEGFR)-3 and integrin α9 were elevated in LAM-LECs. Inhibition of VEGFR-3 suppressed proliferation and migration of LECs, and blockade of integrin α9 reduced VEGF-D-induced migration of LECs. Our data uncovered the distinct features of LAM-associated LECs, increased proliferation and migration, which may be due to higher expression of VEGFR-3 and integrin α9. Furthermore, we also found VEGF-D/VEGFR-3 and VEGF-D/ integrin α9 signaling play an important role in LAM-associated lymphangiogenesis.

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Vascular Endothelial Growth Factor Inhibits the Development of Dendritic Cells and Dramatically Affects the Differentiation of Multiple Hematopoietic Lineages In Vivo

Blood ◽

10.1182/blood.v92.11.4150 ◽

1998 ◽

Vol 92 (11) ◽

pp. 4150-4166 ◽

Cited By ~ 624

Author(s):

Dmitry Gabrilovich ◽

Tadao Ishida ◽

Tsunehiro Oyama ◽

Sophia Ran ◽

Vladimir Kravtsov ◽

...

Keyword(s):

Stem Cells ◽

Vascular Endothelial Growth Factor ◽

Dendritic Cells ◽

Growth Factor ◽

System Control ◽

Vascular Endothelial ◽

Almost All ◽

Endothelial Growth Factor

Abstract Defective function of dendritic cells (DC) in cancer has been recently described and may represent one of the mechanisms of tumor evasion from immune system control. We have previously shown in vitro that vascular endothelial growth factor (VEGF), produced by almost all tumors, is one of the tumor-derived factors responsible for the defective function of these cells. In this study, we investigated whether in vivo infusion of recombinant VEGF could reproduce the observed DC dysfunction. Continuous VEGF infusion, at rates as low as 50 ng/h (resulting in serum VEGF concentrations of 120 to 160 pg/mL), resulted in a dramatic inhibition of dendritic cell development, associated with an increase in the production of B cells and immature Gr-1+ myeloid cells. Infusion of VEGF was associated with inhibition of the activity of the transcription factor NF-κB in bone marrow progenitor cells. Experiments in vitro showed that VEGF itself, and not factors released by VEGF-activated endothelial cells, affected polypotent stem cells resulting in the observed abnormal hematopoiesis. These data suggest that VEGF, at pathologically relevant concentrations in vivo, may exert effects on pluripotent stem cells that result in blocked DC development as well as affect many other hematopoietic lineages.

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