Expression analysis of a high-affinity nitrate transporter isolated from Arabidopsis thaliana by differential display

Planta ◽  
1999 ◽  
Vol 207 (3) ◽  
pp. 461-469 ◽  
Author(s):  
Sophie Filleur ◽  
Françoise Daniel-Vedele
Botany ◽  
2011 ◽  
Vol 89 (9) ◽  
pp. 647-653 ◽  
Author(s):  
Shengjie Bao ◽  
Lijun An ◽  
Sha Su ◽  
Zhongjing Zhou ◽  
Yinbo Gan

Nitrate transporter AtNRT2.1 is the key component of the inducible high-affinity nitrate transport system in Arabidopsis thaliana . AtNRT2.1 is primarily expressed in roots and known to be mainly involved under fluctuating nitrogen supply conditions. It is still unknown whether AtNRT2.1 is involved in plant responses to other nutrient fluctuations. In this study, we found that the expression of AtNRT2.1 was also upregulated by phosphate and sulfate resupply, which may indicate a novel role in regulating phosphate and sulfate responses. Our study also demonstrated that expression of the major Pi transporter (Pht1) family member, AtPHT1;2, was suppressed by nitrate starvation and induced by nitrate resupply and sulfate starvation in comparison to the continuous nutrient treatment. Moreover, this study also showed that expression of sulfur transporter SULTR1;1 and AtNRT2.1 was suppressed by complete nutrient starvation and induced by complete nutrient resupply. These novel results provide strong evidence that there is crosstalk among the nitrate, sulfate, and phosphate transporters in regulating different nutrient fluctuations in Arabidopsis roots.


1999 ◽  
Vol 17 (5) ◽  
pp. 563-568 ◽  
Author(s):  
Degen Zhuo ◽  
Mamoru Okamoto ◽  
J. John Vidmar ◽  
Anthony D. M. Glass

2009 ◽  
Vol 59 (3) ◽  
pp. 237-243 ◽  
Author(s):  
Hisato Katayama ◽  
Mari Mori ◽  
Yoko Kawamura ◽  
Toshinori Tanaka ◽  
Masashi Mori ◽  
...  

2007 ◽  
Vol 104 (47) ◽  
pp. 18807-18812 ◽  
Author(s):  
H. Tomatsu ◽  
J. Takano ◽  
H. Takahashi ◽  
A. Watanabe-Takahashi ◽  
N. Shibagaki ◽  
...  

2021 ◽  
Author(s):  
M. AYDIN AKBUDAK ◽  
Ertugrul Filiz ◽  
Durmus Cetin

High-affinity nitrate transporter 2 (NRT2) proteins have vital roles in nitrate (NO3-) uptake and translocation in plants. The gene families coding NRT2 proteins have been identified and functionally characterized in many plant species. However, no systematic identification of NRT2 family members have been reported in tomato (Solanum lycopersicum). There is also little known about their expression profiles under environmental stresses. Accordingly, the present study aimed to identify NRT2 gene family in the tomato genome; then, investigate them in detail through bioinformatics, physiological and expression analyses. As a result, four novel NRT2 genes were identified in the tomato genome, all of which contain the same domain belonging to the Major Facilitator Superfamily (PF07690). The co-expression network of SlNRT genes revealed that they were co-expressed with several other genes in many different molecular pathways including transport, photosynthesis, fatty acid metabolism and amino acid catabolism. Programming many crucial physiological and metabolic pathways, various numbers of phosphorylation sites were predicted in the NRT2 proteins.


2021 ◽  
Vol 22 (23) ◽  
pp. 13036
Author(s):  
Normig M. Zoghbi-Rodríguez ◽  
Samuel David Gamboa-Tuz ◽  
Alejandro Pereira-Santana ◽  
Luis C. Rodríguez-Zapata ◽  
Lorenzo Felipe Sánchez-Teyer ◽  
...  

Nitrate transporter 2 (NRT2) and NRT3 or nitrate-assimilation-related 2 (NAR2) proteins families form a two-component, high-affinity nitrate transport system, which is essential for the acquisition of nitrate from soils with low N availability. An extensive phylogenomic analysis across land plants for these families has not been performed. In this study, we performed a microsynteny and orthology analysis on the NRT2 and NRT3 genes families across 132 plants (Sensu lato) to decipher their evolutionary history. We identified significant differences in the number of sequences per taxonomic group and different genomic contexts within the NRT2 family that might have contributed to N acquisition by the plants. We hypothesized that the greater losses of NRT2 sequences correlate with specialized ecological adaptations, such as aquatic, epiphytic, and carnivory lifestyles. We also detected expansion on the NRT2 family in specific lineages that could be a source of key innovations for colonizing contrasting niches in N availability. Microsyntenic analysis on NRT3 family showed a deep conservation on land plants, suggesting a high evolutionary constraint to preserve their function. Our study provides novel information that could be used as guide for functional characterization of these gene families across plant lineages.


2005 ◽  
Vol 58 (1) ◽  
pp. 41-51 ◽  
Author(s):  
Anna Paola Casazza ◽  
Silvia Rossini ◽  
Mario G. Rosso ◽  
Carlo Soave

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