Expression patterns of nitrate, phosphate, and sulfate transporters in Arabidopsis roots exposed to different nutritional regimes

Nitrate transporter AtNRT2.1 is the key component of the inducible high-affinity nitrate transport system in Arabidopsis thaliana . AtNRT2.1 is primarily expressed in roots and known to be mainly involved under fluctuating nitrogen supply conditions. It is still unknown whether AtNRT2.1 is involved in plant responses to other nutrient fluctuations. In this study, we found that the expression of AtNRT2.1 was also upregulated by phosphate and sulfate resupply, which may indicate a novel role in regulating phosphate and sulfate responses. Our study also demonstrated that expression of the major Pi transporter (Pht1) family member, AtPHT1;2, was suppressed by nitrate starvation and induced by nitrate resupply and sulfate starvation in comparison to the continuous nutrient treatment. Moreover, this study also showed that expression of sulfur transporter SULTR1;1 and AtNRT2.1 was suppressed by complete nutrient starvation and induced by complete nutrient resupply. These novel results provide strong evidence that there is crosstalk among the nitrate, sulfate, and phosphate transporters in regulating different nutrient fluctuations in Arabidopsis roots.

Download Full-text

The Mitochondrial Phosphate Transporters Modulate Plant Responses to Salt Stress via Affecting ATP and Gibberellin Metabolism in Arabidopsis thaliana

PLoS ONE ◽

10.1371/journal.pone.0043530 ◽

2012 ◽

Vol 7 (8) ◽

pp. e43530 ◽

Cited By ~ 47

Author(s):

Wei Zhu ◽

Qing Miao ◽

Dan Sun ◽

Guodong Yang ◽

Changai Wu ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Salt Stress ◽

Plant Responses ◽

Phosphate Transporters ◽

Gibberellin Metabolism

Download Full-text

Phylogenomic and Microsynteny Analysis Provides Evidence of Genome Arrangements of High-Affinity Nitrate Transporter Gene Families of Plants

International Journal of Molecular Sciences ◽

10.3390/ijms222313036 ◽

2021 ◽

Vol 22 (23) ◽

pp. 13036

Author(s):

Normig M. Zoghbi-Rodríguez ◽

Samuel David Gamboa-Tuz ◽

Alejandro Pereira-Santana ◽

Luis C. Rodríguez-Zapata ◽

Lorenzo Felipe Sánchez-Teyer ◽

...

Keyword(s):

Functional Characterization ◽

Nitrate Assimilation ◽

Gene Families ◽

Land Plants ◽

Nitrate Transport ◽

Phylogenomic Analysis ◽

Evolutionary Constraint ◽

N Availability ◽

Nitrate Transporter ◽

High Affinity

Nitrate transporter 2 (NRT2) and NRT3 or nitrate-assimilation-related 2 (NAR2) proteins families form a two-component, high-affinity nitrate transport system, which is essential for the acquisition of nitrate from soils with low N availability. An extensive phylogenomic analysis across land plants for these families has not been performed. In this study, we performed a microsynteny and orthology analysis on the NRT2 and NRT3 genes families across 132 plants (Sensu lato) to decipher their evolutionary history. We identified significant differences in the number of sequences per taxonomic group and different genomic contexts within the NRT2 family that might have contributed to N acquisition by the plants. We hypothesized that the greater losses of NRT2 sequences correlate with specialized ecological adaptations, such as aquatic, epiphytic, and carnivory lifestyles. We also detected expansion on the NRT2 family in specific lineages that could be a source of key innovations for colonizing contrasting niches in N availability. Microsyntenic analysis on NRT3 family showed a deep conservation on land plants, suggesting a high evolutionary constraint to preserve their function. Our study provides novel information that could be used as guide for functional characterization of these gene families across plant lineages.

Download Full-text

Arabidopsis thaliana High-Affinity Phosphate Transporters Exhibit Multiple Levels of Posttranslational Regulation

The Plant Cell ◽

10.1105/tpc.110.081067 ◽

2011 ◽

Vol 23 (4) ◽

pp. 1523-1535 ◽

Cited By ~ 105

Author(s):

Vincent Bayle ◽

Jean-François Arrighi ◽

Audrey Creff ◽

Claude Nespoulous ◽

Jérôme Vialaret ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Posttranslational Regulation ◽

Phosphate Transporters ◽

High Affinity ◽

Multiple Levels

Download Full-text

Regulation of a putative high‐affinity nitrate transporter ( Nrt2;1At ) in roots of Arabidopsis thaliana

The Plant Journal ◽

10.1046/j.1365-313x.1999.00396.x ◽

1999 ◽

Vol 17 (5) ◽

pp. 563-568 ◽

Cited By ~ 169

Author(s):

Degen Zhuo ◽

Mamoru Okamoto ◽

J. John Vidmar ◽

Anthony D. M. Glass

Keyword(s):

Arabidopsis Thaliana ◽

Nitrate Transporter ◽

High Affinity

Download Full-text

Expression analysis of a high-affinity nitrate transporter isolated from Arabidopsis thaliana by differential display

Planta ◽

10.1007/s004250050505 ◽

1999 ◽

Vol 207 (3) ◽

pp. 461-469 ◽

Cited By ~ 76

Author(s):

Sophie Filleur ◽

Françoise Daniel-Vedele

Keyword(s):

Arabidopsis Thaliana ◽

Expression Analysis ◽

Differential Display ◽

Nitrate Transporter ◽

High Affinity

Download Full-text

Faculty Opinions recommendation of An Arabidopsis thaliana high-affinity molybdate transporter required for efficient uptake of molybdate from soil.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1095962.555453 ◽

2008 ◽

Author(s):

Ulf-Ingo Flugge

Keyword(s):

Arabidopsis Thaliana ◽

High Affinity ◽

Efficient Uptake

Download Full-text

Characterization of Interactions between the Soybean Salt-Stress Responsive Membrane-Intrinsic Proteins GmPIP1 and GmPIP2

Agronomy ◽

10.3390/agronomy11071312 ◽

2021 ◽

Vol 11 (7) ◽

pp. 1312

Author(s):

Jia Liu ◽

Weicong Qi ◽

Haiying Lu ◽

Hongbo Shao ◽

Dayong Zhang

Keyword(s):

Plasma Membrane ◽

Salt Stress ◽

Salt Tolerance ◽

Expression Profiles ◽

Expression Patterns ◽

Gene Expression Profiles ◽

Early Gene ◽

Plant Responses ◽

Constitutive Overexpression ◽

Important Trait

Salt tolerance is an important trait in soybean cultivation and breeding. Plant responses to salt stress include physiological and biochemical changes that affect the movement of water across the plasma membrane. Plasma membrane intrinsic proteins (PIPs) localize to the plasma membrane and regulate the water and solutes flow. In this study, quantitative real-time PCR and yeast two-hybridization were engaged to analyze the early gene expression profiles and interactions of a set of soybean PIPs (GmPIPs) in response to salt stress. A total of 20 GmPIPs-encoding genes had varied expression profiles after salt stress. Among them, 13 genes exhibited a downregulated expression pattern, including GmPIP1;6, the constitutive overexpression of which could improve soybean salt tolerance, and its close homologs GmPIP1;7 and 1;5. Three genes showed upregulated patterns, including the GmPIP1;6 close homolog GmPIP1;4, when four genes with earlier increased and then decreased expression patterns. GmPIP1;5 and GmPIP1;6 could both physically interact strongly with GmPIP2;2, GmPIP2;4, GmPIP2;6, GmPIP2;8, GmPIP2;9, GmPIP2;11, and GmPIP2;13. Definite interactions between GmPIP1;6 and GmPIP1;7 were detected and GmPIP2;9 performed homo-interaction. The interactions of GmPIP1;5 with GmPIP2;11 and 2;13, GmPIP1;6 with GmPIP2;9, 2;11 and GmPIP2;13, and GmPIP2;9 with itself were strengthened upon salt stress rather than osmotic stress. Taken together, we inferred that GmPIP1 type and GmPIP2 type could associate with each other to synergistically function in the plant cell; a salt-stress environment could promote part of their interactions. This result provided new clues to further understand the soybean PIP–isoform interactions, which lead to potentially functional homo- and heterotetramers for salt tolerance.

Download Full-text

Two Expansin Genes, AtEXPA4 and AtEXPB5, Are Redundantly Required for Pollen Tube Growth and AtEXPA4 Is Involved in Primary Root Elongation in Arabidopsis thaliana

Genes ◽

10.3390/genes12020249 ◽

2021 ◽

Vol 12 (2) ◽

pp. 249

Author(s):

Weimiao Liu ◽

Liai Xu ◽

Hui Lin ◽

Jiashu Cao

Keyword(s):

Arabidopsis Thaliana ◽

Pollen Tube ◽

Pollen Tube Growth ◽

Cell Walls ◽

Root Elongation ◽

Expression Patterns ◽

Primary Root ◽

Pollen Grains ◽

Tube Growth ◽

Cell Wall Binding

The growth of plant cells is inseparable from relaxation and expansion of cell walls. Expansins are a class of cell wall binding proteins, which play important roles in the relaxation of cell walls. Although there are many members in expansin gene family, the functions of most expansin genes in plant growth and development are still poorly understood. In this study, the functions of two expansin genes, AtEXPA4 and AtEXPB5 were characterized in Arabidopsis thaliana. AtEXPA4 and AtEXPB5 displayed consistent expression patterns in mature pollen grains and pollen tubes, but AtEXPA4 also showed a high expression level in primary roots. Two single mutants, atexpa4 and atexpb5, showed normal reproductive development, whereas atexpa4atexpb5 double mutant was defective in pollen tube growth. Moreover, AtEXPA4 overexpression enhanced primary root elongation, on the contrary, knocking out AtEXPA4 made the growth of primary root slower. Our results indicated that AtEXPA4 and AtEXPB5 were redundantly involved in pollen tube growth and AtEXPA4 was required for primary root elongation.

Download Full-text