Biochar Phosphate Fertilizer Loaded with Urea Preserves Available Nitrogen Longer than Conventional Urea

Biochar, a carbon-rich material obtained by pyrolysis of organic wastes, is an attractive matrix for loading nutrients and producing enhanced efficiency fertilizers. In this study, poultry litter (PL) was enriched with phosphoric acid (H3PO4) and MgO to produce a biochar-based fertilizer (PLB), which was loaded with urea in a 4:5 ratio (PLB:urea, w/w) to generate a 15–15% N–P slow-release fertilizer (PLB–N) to be used in a single application to soil. A greenhouse experiment was carried out in which a common bean was cultivated followed by maize to evaluate the agronomic efficiency and the residual effect of fertilization with PLB–N in Ultisol. Six treatments were tested, including four doses of N (100, 150, 200, and 250 mg kg−1) via PLB–N in a single application, a control with triple superphosphate (TSP—applied once) and urea (split three times), and a control without N-P fertilization. The greatest effect of PLB–N was the residual effect of fertilization, in which maize showed a linear response to the N doses applied via PLB–N but showed no response to conventional TSP + urea fertilization. Biochar has the potential as a loading matrix to preserve N availability and increase residual effects and N-use efficiency by plants.

Effect of 50 Years of No-Tillage, Stubble Retention, and Nitrogen Fertilization on Soil Respiration, Easily Extractable Glomalin, and Nitrogen Mineralization

In subtropical regions, we have an incomplete understanding of how long-term tillage, stubble, and nitrogen (N) fertilizer management affects soil biological functioning. We examined a subtropical site managed for 50 years using varying tillage (conventional till (CT) and no-till (NT)), stubble management (stubble burning (SB) and stubble retention (SR)), and N fertilization (0 (N0), 30 (N30), and 90 (N90) kg ha−1 y−1) to assess their impact on soil microbial respiration, easily extractable glomalin-related soil protein (EEGRSP), and N mineralization. A significant three-way tillage × stubble × N fertilizer interaction was observed for soil respiration, with NT+SB+N0 treatments generally releasing the highest amounts of CO2 over the incubation period (1135 mg/kg), and NT+SR+N0 treatments releasing the lowest (528 mg/kg). In contrast, a significant stubble × N interaction was observed for both EEGRSP and N mineralization, with the highest concentrations of both EEGRSP (2.66 ± 0.86 g kg−1) and N mineralization (30.7 mg/kg) observed in SR+N90 treatments. Furthermore, N mineralization was also positively correlated with EEGRSP (R2 = 0.76, p < 0.001), indicating that EEGRSP can potentially be used as an index of soil N availability. Overall, this study has shown that SR and N fertilization have a positive impact on soil biological functioning.

Reducing Tillage Affects Long-Term Yields but Not Grain Quality of Maize, Soybeans, Oats, and Wheat Produced in Three Contrasting Farming Systems

Reducing tillage has been widely promoted to reduce soil erosion, maintain soil health, and sustain long-term food production. The effects of reducing tillage on crop nutritional quality in organic and conventional systems, however, has not been widely explored. One possible driver of crop nutritional quality might be the changing soil nitrogen (N) availability associated with reduced tillage in various management systems. To test how reducing tillage affects crop nutritional quality under contrasting conventional and organic farming systems with varied N inputs, we measured nutritional quality (protein, fat, starch, ash, net energy, total digestible nutrients, and concentrations of Ca, K, Mg, P, and S) of maize, wheat, oats, and soybeans harvested from a long-term trial comprised of three farming systems under two tillage regimes: a conventional grain system (CNV); a low-input organic grain system (LEG); and an organic, manure-based grain + forage system (MNR) under conventional full-tillage (FT) and reduced-till (RT) management. Although maize and wheat yields were 10–13% lower under RT management, grain quality metrics including protein, fat, starch, energy, and mineral concentrations were not significantly affected by reducing tillage. Differences in nutrient quality were more marked between farming systems: protein levels in maize were highest in the MNR system (8.1%); protein levels in soybeans were highest in the LEG system (40.4%); levels of protein (12.9%), ash (2.0%), and sulfur (1430 ppm) in wheat were highest in the CNV system, and oat quality was largely consistent between the LEG and MNR systems. As grain quality did not significantly respond to reducing tillage, other management decisions that affect nutrient availability appear to have a greater effect on nutrient quality.

Foliar and root uptake of N deriving from simulated atmospheric N depositions in potted apple (Malus domestica) trees

A significant human-driven increment of the available reactive nitrogen (Nr) forms has occurred during the past century at the global scale, which in turn has increased the amount of Nr deposition. Grafted apple trees (Gala / M.9 strain T337) were used in a pot experiment conducted in semicontrolled conditions, where the 15N-labelling technique allowed to trace the fate of N from ammonium nitrate (15NH4 15NO3, isotopic enrichment: 10.3 atoms %) distributed at three increasing rates (N1, N2, N4, where N2 is the double of N1 and N4 is the double of N2) either to soil or to canopy (foliar application) to simulate atmospheric N depositions. At the end of the experiment, plants were destructively sampled, and N derived from depositions (Ndfd), total N, and biomass of above and belowground organs were determined. Uptake rates ranged from 21% to 57% and the Ndfd recovery was higher for soil than for foliar application. Foliar-supplied plants showed a higher Ndfd in leaves and shoots than soil-supplied ones, while the latter showed a higher Ndfd in roots than the former. Moreover, total N in trunk, shoot axes and leaves increased with the N rates up to the level N2, with no further increase in N4. Increasing tree N availability, regardless the supply mode, increased the shoot:root N content. The fact that the N uptake rate was rather stable at increasing N rates suggests that if N from atmospheric depositions becomes increasingly available at the canopy or soil level, it will actively contribute to apple tree nutrition and account for a significant fraction of the apple tree N needs.

Use of Digestate as Organic Amendment and Source of Nitrogen to Vegetable Crops

Anaerobic digestion is a valuable process to use livestock effluents to produce green energy and a by-product called digestate with fertilising value. This work aimed at evaluating the fertilising value of the solid fraction (SF) of a digestate as an organic amendment and as a source of nitrogen to crops replacing mineral N. A field experiment was done with two consecutive vegetable crops. The treatments were: a control without fertilisation; Ni85 mineral fertilisation with 85 kg ha−1 of mineral N; fertiliser with digestate at an increasing nitrogen application rate (kg N ha−1): DG-N85 DG-N170, DG-N170+85, DG-N170+170; fertilisation with digestate together with Ni: DG-N85+Ni60, DG-N170+Ni60, DG-N170+Ni25. The results showed a soil organic amendment effect of the SF with a beneficial effect on SOM, soil pH and exchangeable bases. The SF was able to replace part of the mineral N fertilisation. The low mineralisation of the stable organic matter together with some immobilisation of mineral N from SF caused low N availability. The fertilisation planning should consider the SF ratio between the organic N (NO) and total N (TKN). Low NO:TKN ratios (≈0.65) needed lower Ni addition to maintaining the biomass production similar to the mineral fertilisation.

Foliar Application of Protein Hydrolysates on Baby-Leaf Spinach Grown at Different N Levels

Surpluses of N are associated with environmental and health problems. To optimise N use and reduce nitrate accumulation in leafy species like spinach, the application of biostimulants is suggested. An experiment in controlled conditions (growth chamber/soilless) evaluated baby-spinach responses to two protein hydrolysates (PHs) from plant (legume, Trainer®) and animal (meat, Isabion®) sources, combined with three N rates: 2 (N2, deficient), 8 (N8, sub-optimal), and 14 (N14, optimal) mM of N. Biometrical and morphological traits of shoots and roots as well as the physio-metabolic (gas exchange, N assimilation, and NUtE), physical, mineral, and antioxidant profiles of leaves were assessed. The legume-PH boosts growth and yield only at the highest N conditions, while there was no effect at lower N rates. The legume-PH modulates root architecture and chlorophylls has positive responses only at optimal N availability, such as an increase in N uptake, leaf expansion, and photosynthetic activity at the canopy level. The PHs do not improve NUtE, leaf colour, consistency, cations, or antioxidants. Neither do PHs have any effect on reducing nitrate accumulation. Legume-PH improves N assimilation only at optimal N availability, while meat-PH does not, reaching the highest nitrate value at the highest N rate (2677 mg kg−1 fw), even if this value is under the EC limits for fresh spinach.

Nitrogen Fertiliser Immobilisation and Uptake in the Rhizospheres of Wheat and Canola

Immobilisation of fertiliser nitrogen (N) by soil microorganisms can reduce N availability to crops, decreasing growth and yield. To date, few studies have focussed on the effect of different plant species on immobilisation of fertiliser N. Canola (Brassica napus) is known to influence the soil microbiome and increase mineral N in soil for future crops compared with cereals. We tested the hypothesis that canola can reduce immobilisation of fertiliser N by influencing the composition of the rhizosphere microbiome. To investigate this, we conducted a glasshouse soil column experiment comparing N fertiliser uptake between canola and wheat (Triticum aestivium) and partitioning of fertiliser N between plants and microorganisms. Plants were grown in soil to which high C:N ratio wheat residues and 15N-labelled urea fertiliser were applied. There was no difference between wheat and canola in fertiliser N uptake despite differences in fungal community composition and the carbon metabolising enzyme alpha-glucosidase in the rhizosphere. Canola obtained more soil-derived N than wheat. There was no significant difference in the rhizosphere bacterial communities present between wheat and canola and unplanted controls. Our results highlight the capacity of canola to increase mineralisation of soil N compared with wheat although the study could not describe the microbial community which facilitated this increase.

Phylogenomic and Microsynteny Analysis Provides Evidence of Genome Arrangements of High-Affinity Nitrate Transporter Gene Families of Plants

Nitrate transporter 2 (NRT2) and NRT3 or nitrate-assimilation-related 2 (NAR2) proteins families form a two-component, high-affinity nitrate transport system, which is essential for the acquisition of nitrate from soils with low N availability. An extensive phylogenomic analysis across land plants for these families has not been performed. In this study, we performed a microsynteny and orthology analysis on the NRT2 and NRT3 genes families across 132 plants (Sensu lato) to decipher their evolutionary history. We identified significant differences in the number of sequences per taxonomic group and different genomic contexts within the NRT2 family that might have contributed to N acquisition by the plants. We hypothesized that the greater losses of NRT2 sequences correlate with specialized ecological adaptations, such as aquatic, epiphytic, and carnivory lifestyles. We also detected expansion on the NRT2 family in specific lineages that could be a source of key innovations for colonizing contrasting niches in N availability. Microsyntenic analysis on NRT3 family showed a deep conservation on land plants, suggesting a high evolutionary constraint to preserve their function. Our study provides novel information that could be used as guide for functional characterization of these gene families across plant lineages.

Biotechnological approaches to develop nitrogen-fixing cereals: A review

Agricultural yields are often limited by nitrogen (N) availability, especially in countries of the developing world, whereas in industrialized nations the application of chemical N fertilizers has reached unsustainable levels that have resulted in severe environmental consequences. Finding alternatives to inorganic fertilizers is critical for sustainable and secure food production. Although gaseous nitrogen (N2) is abundant in the atmosphere, it cannot be assimilated by most living organisms. Only a selected group of microorganisms termed diazotrophs, have evolved the ability to reduce N2 to generate NH3 in a process known as biological nitrogen fixation (BNF) catalysed by nitrogenase, an oxygen-sensitive enzyme complex. This ability presents an opportunity to improve the nutrition of crop plants, through the introduction into cereal crops of either the N fixing bacteria or the nitrogenase enzyme responsible for N fixation. This review explores three potential approaches to obtain N-fixing cereals: (a) engineering the nitrogenase enzyme to function in plant cells; (b) engineering the legume symbiosis into cereals; and (c) engineering cereals with the capability to associate with N-fixing bacteria.