First molecular evidence of the simultaneous human infection with two species of Echinococcus granulosus sensu lato: Echinococcus granulosus sensu stricto and Echinococcus canadensis

Abstract Echinococcus granulosus sensu stricto is regarded to have the highest zoonotic potential of all Echinococcus taxa. Globally, human infection due to this species constitutes over 88.44% of the total cystic echinococcosis (CE) burden. Here, we report a CE infection in a Nigerian camel caused by E. granulosus G1 genotype. To the best of our knowledge, this report is the first encounter of the G1 genotype in the West Africa sub-region where the G6 genotype is reportedly prevalent, suggesting that the epidemiology of this highly zoonotic group could have a wider host range and distribution in the sub-region, and emphasizes the need for further investigation into the genetic diversity of Echinococcus spp. in Nigeria and across the sub-region.

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A multiplex PCR for differential detection of Echinococcus granulosus sensu stricto, Echinococcus multilocularis and Echinococcus canadensis in China

Infectious Diseases of Poverty ◽

10.1186/s40249-019-0580-2 ◽

2019 ◽

Vol 8 (1) ◽

Cited By ~ 2

Author(s):

Jing-Ye Shang ◽

Guang-Jia Zhang ◽

Sha Liao ◽

Yan Huang ◽

Wen-Jie Yu ◽

...

Keyword(s):

Multiplex Pcr ◽

Echinococcus Granulosus ◽

Echinococcus Multilocularis ◽

Sensu Stricto ◽

Differential Detection ◽

Echinococcus Canadensis ◽

Echinococcus Granulosus Sensu Stricto

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Molecular characterization of Echinococcus granulosus sensu stricto and Echinococcus canadensis in humans and livestock from Algeria

Parasitology Research ◽

10.1007/s00436-016-4994-5 ◽

2016 ◽

Vol 115 (6) ◽

pp. 2423-2431 ◽

Cited By ~ 12

Author(s):

Houria Zait ◽

Mokhtaria Kouidri ◽

Florence Elisabeth Grenouillet ◽

Gérald Umhang ◽

Laurence Millon ◽

...

Keyword(s):

Molecular Characterization ◽

Echinococcus Granulosus ◽

Sensu Stricto ◽

Echinococcus Canadensis ◽

Echinococcus Granulosus Sensu Stricto

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Echinococcus canadensis (G7) and Echinococcus granulosus sensu stricto (G1) in swine of southern Brazil

Veterinary Parasitology ◽

10.1016/j.vetpar.2014.01.023 ◽

2014 ◽

Vol 202 (3-4) ◽

pp. 335-338 ◽

Cited By ~ 13

Author(s):

D.U. Monteiro ◽

S.A. Botton ◽

A.A. Tonin ◽

M.I. Azevedo ◽

D.A.S. Graichen ◽

...

Keyword(s):

Echinococcus Granulosus ◽

Sensu Stricto ◽

Southern Brazil ◽

Echinococcus Canadensis ◽

Echinococcus Granulosus Sensu Stricto

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Echinococcus granulosus sensu stricto, Echinococcus ortleppi; and E. intermedius (G7) are present in Bolivia

Parasitology ◽

10.1017/s0031182020000529 ◽

2020 ◽

Vol 147 (9) ◽

pp. 949-956 ◽

Cited By ~ 2

Author(s):

V. Ali ◽

E. Martinez ◽

P. Duran ◽

M. A. Seláez ◽

M. Barragan ◽

...

Keyword(s):

Detailed Analysis ◽

Echinococcus Granulosus ◽

Cystic Echinococcosis ◽

Sensu Stricto ◽

Control Measures ◽

Santa Cruz ◽

La Paz ◽

Echinococcus Granulosus Sensu Stricto ◽

Faecal Samples ◽

Echinococcus Granulosus Sensu Lato

AbstractCystic echinococcosis (CE) is a zoonotic disease caused by a complex of species known as Echinococcus granulosus sensu lato. CE is endemic in Argentina, Chile, Peru, Uruguay and the South part of Brazil. In contrast, little is known regarding the presence of CE in Bolivia. In this study, 35 cysts isolated from livestock (mostly from the Department of La Paz) and 3 from humans (La Paz, Oruro and Potosi) were genetically characterized analysing the sequence of the cox1 gene (1609 bp). In total, 30 cysts (from La Paz, Cochabamba and Beni) were characterized as E. granulosus sensu stricto (3 fertile and 4 non-fertile cysts from sheep, 8 fertile and 12 non-fertile cysts from cattle and 3 fertile cysts from humans). A detailed analysis of the cox1 haplotypes of E. granulosus s.s. is included. Echinococcus ortleppi (G5) was found in 5 fertile cysts from cattle (from La Paz and Cochabamba). Echinococcus intermedius (G7) was identified in 3 fertile cysts from pigs (from Santa Cruz). Additionally, E. granulosus s.s. was detected in 4 dog faecal samples, while E. ortleppi was present in other two dog faecal samples. The implications of these preliminary results in the future implementation of control measures are discussed.

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Molecular identification of cystic echinococcosis in humans and pigs reveals the presence of both Echinococcus granulosus sensu stricto and Echinococcus canadensis G6/G7 in the hyperendemic focus of the Republic of Moldova

Parasitology Research ◽

10.1007/s00436-019-06432-5 ◽

2019 ◽

Vol 118 (10) ◽

pp. 2857-2861

Author(s):

Gérald Umhang ◽

Oleg Chihai ◽

Vanessa Bastid ◽

Florence Elisabeth Grenouillet ◽

Dumitru Erhan ◽

...

Keyword(s):

Echinococcus Granulosus ◽

Molecular Identification ◽

Cystic Echinococcosis ◽

Sensu Stricto ◽

Echinococcus Canadensis ◽

Republic Of Moldova ◽

Echinococcus Granulosus Sensu Stricto ◽

The Republic

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Identification of Echinococcus granulosus Genotypes G1 and G3 by SNPs Genotyping Assays

Pathogens ◽

10.3390/pathogens10020125 ◽

2021 ◽

Vol 10 (2) ◽

pp. 125

Author(s):

Piero Bonelli ◽

Silvia Dei Giudici ◽

Angela Peruzzu ◽

Lorena Mura ◽

Cinzia Santucciu ◽

...

Keyword(s):

Echinococcus Granulosus ◽

Animal Species ◽

Cost Effective ◽

Sensu Stricto ◽

Sybr Green ◽

Hydatid Cysts ◽

Genotype Identification ◽

Host Selectivity ◽

Nad5 Gene ◽

Echinococcus Granulosus Sensu Lato

Echinococcus granulosus sensu lato (s.l.) is the causative agent of cystic echinococcosis in animals and humans. Different E. granulosuss.l. genotypes exhibit great diversity in their life cycle, host selectivity and pathogenicity. For this reason, the study of genetic variation within Echinococcus species is of importance for their epidemiological implication. We employed two SNP genotyping technologies to distinguish G1 and G3 E. granulosus sensu stricto (s.s.). genotypes. The genotypes of DNA samples (n = 28) extracted from hydatid cysts of different animal species were identified by amplification and sequencing of a fragment of the mitochondrial nad5 gene. Two SYBR green and three TaqMan real time PCR assays were developed for targeting of three nad5 informative positions (SNP758, 1123, and 1380) known to be able to discriminate G1 from G3. Genotyping by SYBR Green PCR based on cycle threshold (Ct) with melting temperature (Tm) analysis and performed on SNP1123 and SNP1380 failed to identify one DNA sample. TaqMan assays for SNP758, 1123 and 1380 effectively confirmed genotype identification obtained by Sanger sequencing. Our results demonstrated that the combination of the three Taqman assays developed in this study represents a valuable and cost effective tool alternative to DNA sequencing for E. granulosus s.s. genotyping.

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