Physical mapping and microsynteny of Brassica rapa ssp. pekinensis genome corresponding to a 222 kbp gene-rich region of Arabidopsis chromosome 4 and partially duplicated on chromosome 5

The major difference between annual and biennial cultivars of oilseed Brassica napus and B. rapa is conferred by genes controlling vernalization-responsive flowering time. These genes were compared between the species by aligning the map positions of flowering time quantitative trait loci (QTLs) detected in a segregating population of each species. The results suggest that two major QTLs identified in B. rapa correspond to two major QTLs identified in B. napus. Since B. rapa is one of the hypothesized diploid parents of the amphidiploid B. napus, the vernalization requirement of B. napus probably originated from B. rapa. Brassica genes also were compared to flowering time genes in Arabidopsis thaliana by mapping RFLP loci with the same probes in both B. napus and Arabidopsis. The region containing one pair of Brassica QTLs was collinear with the top of chromosome 5 in A. thaliana where flowering time genes FLC, FY and CO are located. The region containing the second pair of QTLs showed fractured collinearity with several regions of the Arabidopsis genome, including the top of chromosome 4 where FRI is located. Thus, these Brassica genes may correspond to two genes (FLC and FRI) that regulate flowering time in the latest flowering ecotypes of Arabidopsis.

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Identification and Physical Localization of Useful Genes and Markers to a Major Gene-Rich Region on Wheat Group1SChromosomes

Genetics ◽

10.1093/genetics/157.4.1735 ◽

2001 ◽

Vol 157 (4) ◽

pp. 1735-1747 ◽

Cited By ~ 5

Author(s):

Devinder Sandhu ◽

Julie A Champoux ◽

Svetlana N Bondareva ◽

Kulvinder S Gill

Keyword(s):

Dna Analysis ◽

Major Gene ◽

Rflp Markers ◽

Rich Region ◽

Wheat Group ◽

Marker Loci ◽

Group 1 Chromosomes ◽

Gene Rich Region ◽

Homeologous Group ◽

Group 1

AbstractThe short arm of Triticeae homeologous group 1 chromosomes is known to contain many agronomically important genes. The objectives of this study were to physically localize gene-containing regions of the group 1 short arm, enrich these regions with markers, and study the distribution of genes and recombination. We focused on the major gene-rich region (“1S0.8 region”) and identified 75 useful genes along with 93 RFLP markers by comparing 35 different maps of Poaceae species. The RFLP markers were tested by gel blot DNA analysis of wheat group 1 nullisomic-tetrasomic lines, ditelosomic lines, and four single-break deletion lines for chromosome arm 1BS. Seventy-three of the 93 markers mapped to group 1 and detected 91 loci on chromosome 1B. Fifty-one of these markers mapped to two major gene-rich regions physically encompassing 14% of the short arm. Forty-one marker loci mapped to the 1S0.8 region and 10 to 1S0.5 region. Two cDNA markers mapped in the centromeric region and the remaining 24 loci were on the long arm. About 82% of short arm recombination was observed in the 1S0.8 region and 17% in the 1S0.5 region. Less than 1% recombination was observed for the remaining 85% of the physical arm length.

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Chromosomal localization of seven HSA3q13→q23 NotI linking clones on chicken microchromosomes: orthology of GGA14 and GGA15 to a gene-rich region of HSA3

Cytogenetic and Genome Research ◽

10.1159/000086381 ◽

2005 ◽

Vol 111 (2) ◽

pp. 128-133 ◽

Cited By ~ 1

Author(s):

A.A. Sazanov ◽

A.L. Sazanova ◽

V.A. Stekol’nikova ◽

A.A. Kozyreva ◽

M.N. Romanov ◽

...

Keyword(s):

Chromosomal Localization ◽

Rich Region ◽

Gene Rich Region

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The highly recombinogenic bz locus lies in an unusually gene-rich region of the maize genome

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.141221898 ◽

2001 ◽

Vol 98 (15) ◽

pp. 8903-8908 ◽

Cited By ~ 67

Author(s):

H. Fu ◽

W. Park ◽

X. Yan ◽

Z. Zheng ◽

B. Shen ◽

...

Keyword(s):

Maize Genome ◽

Rich Region ◽

Gene Rich Region

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A genetic linkage map of rat Chromosome 5 reveals extensive linkage conservation with mouse Chromosome 4

Mammalian Genome ◽

10.1007/bf00360549 ◽

1994 ◽

Vol 5 (4) ◽

pp. 222-224 ◽

Cited By ~ 6

Author(s):

E. Kobayashi ◽

M. Tachibana ◽

H. Ikadai ◽

T. Kunieda

Keyword(s):

Linkage Map ◽

Mouse Chromosome ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

Chromosome 4 ◽

Chromosome 5 ◽

Linkage Conservation

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An altered body plan is conferred on Arabidopsis plants carrying dominant alleles of two genes

Development ◽

10.1242/dev.122.8.2395 ◽

1996 ◽

Vol 122 (8) ◽

pp. 2395-2403 ◽

Cited By ~ 4

Author(s):

B. Grbic ◽

A.B. Bleecker

Keyword(s):

Shoot Apical Meristem ◽

Apical Meristem ◽

Reproductive Development ◽

Body Plan ◽

Chromosome 4 ◽

Chromosome 5 ◽

Present Evidence ◽

Primary Shoot ◽

Axillary Meristems

In this paper, we describe a late-flowering ecotype of Arabidopsis, Sy-0, in which the axillary meristems maintain a prolonged vegetative phase, even though the primary shoot apical meristem has already converted to reproductive development. This novel heterochronic shift in the development of axillary meristems results in the formation of aerial rosettes of leaves at the nodes of the primary shoot axis. We present evidence that the aerial-rosette phenotype arises due to the interaction between dominant alleles of two genes: ART, aerial rosette gene (on chromosome 5) and EAR, enhancer of aerial rosette (on chromosome 4): EAR has been tentatively identified as a new allele of the FRI locus. The possible role of these two genes in the conversion of shoot apical meristems to reproductive development is discussed.

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Rps 8 Maps to a Resistance Gene Rich Region on Soybean Molecular Linkage Group F

Crop Science ◽

10.2135/cropsci2004.04-0024 ◽

2006 ◽

Vol 46 (1) ◽

pp. 168-173 ◽

Cited By ~ 56

Author(s):

Stuart G. Gordon ◽

Steven K. St. Martin ◽

Anne E. Dorrance

Keyword(s):

Linkage Group ◽

Resistance Gene ◽

Rich Region ◽

Molecular Linkage Group ◽

Gene Rich Region

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Chromosomal rearrangements in rock wallabies, Petrogale (Marsupialia: Macropodidae). III. G-banding analysis of Petrogale inornata and P. penicillata

Genome ◽

10.1139/g90-120 ◽

1990 ◽

Vol 33 (6) ◽

pp. 798-802 ◽

Cited By ~ 11

Author(s):

M. D. B. Eldridge ◽

R. L. Close ◽

P. G. Johnston

Keyword(s):

Acrocentric Chromosome ◽

Chromosomal Rearrangements ◽

Paracentric Inversion ◽

Chromosome 3 ◽

Chromosome 4 ◽

Chromosome 5 ◽

Cultured Fibroblasts ◽

Submetacentric Chromosome ◽

Ancestral Chromosome ◽

Banding Analysis

The karyotypes of Petrogale inornata and the two currently recognised races of Petrogale penicillata were examined using G-banding from cultured fibroblasts. Petrogale inornata (2n = 22) was found to retain plesiomorphic chromosomes 3 and 4 but possessed an apomorphic inverted chromosome 5 (5i). This 5i appears identical with the 5i found in two other Queensland taxa, Petrogale assimilis and Petrogale godmani, and can be derived from the ancestral chromosome 5 by an extensive paracentric inversion or a centromeric transposition. Petrogale penicillata penicillata (2n = 22) and Petrogale penicillata herberti (2n = 22) both possess the synapomorphic acrocentric chromosome 3, which appears to differ from the plesiomorphic 3 by a small centromeric transposition. Petrogale p. penicillata was also found to be characterised by an apomorphic acrocentric chromosome 4, while P. p. herberti was characterised by an autapomorphic submetacentric chromosome 4. Both apomorphic chromosomes 4 can be related to the plesiomorphic chromosome 4 by centromeric transpositions. Thus although P. inornata is chromosomally distinct it is more closely related to other north Queensland taxa than it is to either P. p. penicillata or P. p. herberti.Key words: chromosomal rearrangements, G-banding, Marsupialia, Petrogale, Macropodidae.

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