Transposition of the piggyBac element in embryos of Drosophila melanogaster, Aedes aegypti and Trichoplusia ni

1999 ◽  
Vol 261 (4-5) ◽  
pp. 803-810 ◽  
Author(s):  
N. Lobo ◽  
X. Li ◽  
M. J. Fraser
Keyword(s):  
Drosophila Melanogaster ◽  
Aedes Aegypti ◽  
Trichoplusia Ni

Structure, Expression, and Hormonal Control of Genes from the Mosquito, Aedes aegypti, Which Encode Proteins Similar to the Vitelline Membrane Proteins of Drosophila melanogaster

1993 ◽  
Vol 155 (2) ◽  
pp. 558-568 ◽  
Author(s):  
Yonggu Lin ◽  
Martha T. Hamblin ◽  
Marten J. Edwards ◽  
Carolina Barillas-Mury ◽  
Michael R. Kanost ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Membrane Proteins ◽  
Aedes Aegypti ◽  
Hormonal Control ◽  
Vitelline Membrane

Recombinant expression of Drosophila melanogaster α-l-fucosidase in Trichoplusia ni cells

2011 ◽  
Vol 57 (9) ◽  
pp. 1205-1211 ◽  
Author(s):  
Yipeng Xu ◽  
Jari Intra ◽  
Chuan-xi Zhang ◽  
Maria Enrica Pasini
Keyword(s):  
Drosophila Melanogaster ◽  
Trichoplusia Ni ◽  
Recombinant Expression

Transgenic expression of the Aedes aegypti CYP9J28 confers pyrethroid resistance in Drosophila melanogaster

2012 ◽  
Vol 104 (2) ◽  
pp. 132-135 ◽  
Author(s):  
N. Pavlidi ◽  
M. Monastirioti ◽  
P. Daborn ◽  
I. Livadaras ◽  
T. Van Leeuwen ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Aedes Aegypti ◽  
Pyrethroid Resistance ◽  
Transgenic Expression

scholarly journals A wMel Wolbachia variant in Aedes aegypti from field-collected Drosophila melanogaster with increased phenotypic stability under heat stress

2022 ◽  
Author(s):  
Xinyue Gu ◽  
Perran A Ross ◽  
Julio Rodriguez-Andres ◽  
Katie L. Robinson ◽  
Qiong Yang ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Stress ◽  
Aedes Aegypti ◽  
Cytoplasmic Incompatibility ◽  
Vector Competence ◽  
Temperature Tolerance ◽  
Seasonal Temperature ◽  
Maternal Transmission ◽  
Tropical Regions ◽  
Host Fitness

Mosquito-borne diseases such as dengue, Zika and chikungunya remain a major cause of morbidity and mortality across tropical regions. Population replacement strategies involving the wMel strain of Wolbachia are being used widely to control mosquito-borne diseases transmitted by Aedes aegypti. However, these strategies may be influenced by environmental temperature because wMel is vulnerable to heat stress. wMel infections in their native host Drosophila melanogaster are genetically diverse, but few transinfections of wMel variants have been generated in Ae. aegypti mosquitoes. Here we successfully transferred a wMel variant (termed wMelM) originating from a field-collected D. melanogaster population from Victoria, Australia into Ae. aegypti. The new wMelM variant (clade I) is genetically distinct from the original wMel transinfection (clade III) generated over ten years ago, and there are no genomic differences between wMelM in its original and transinfected host. We compared wMelM with wMel in its effects on host fitness, temperature tolerance, Wolbachia density, vector competence, cytoplasmic incompatibility and maternal transmission under heat stress in a controlled background. wMelM showed a higher heat tolerance than wMel, with stronger cytoplasmic incompatibility and maternal transmission when eggs were exposed to heat stress, likely due to higher overall densities within the mosquito. Both wMel variants had minimal host fitness costs, complete cytoplasmic incompatibility and maternal transmission, and dengue virus blocking under standard laboratory conditions. Our results highlight phenotypic differences between closely related Wolbachia variants. wMelM shows potential as an alternative strain to wMel in dengue control programs in areas with strong seasonal temperature fluctuations.

Bursicon homodimers induce prophylactic innate immunity in two model insects: Drosophila melanogaster and Aedes aegypti

2016 ◽  
Author(s):  
◽  
Hongwei Zhang
Keyword(s):  
Innate Immunity ◽  
Drosophila Melanogaster ◽  
Aedes Aegypti ◽  
University Of Missouri ◽  
The University

[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.]

scholarly journals IPB7 transposase behavior in Drosophila melanogaster and Aedes aegypti

2013 ◽  
Vol 43 (10) ◽  
pp. 899-906 ◽  
Author(s):  
Jennifer A. Wright ◽  
Ryan C. Smith ◽  
Xianghong Li ◽  
Nancy L. Craig ◽  
Peter W. Atkinson
Keyword(s):  
Drosophila Melanogaster ◽  
Aedes Aegypti

scholarly journals The Aedes aegypti Domino Ortholog p400 Regulates Antiviral Exogenous Small Interfering RNA Pathway Activity and ago-2 Expression

mSphere ◽  
2020 ◽  
Vol 5 (2) ◽  
Author(s):  
Melanie McFarlane ◽  
Floriane Almire ◽  
Joy Kean ◽  
Claire L. Donald ◽  
Alma McDonald ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Antiviral Activity ◽  
Aedes Aegypti ◽  
Small Interfering Rna ◽  
Antiviral Response ◽  
Pathway Activity ◽  
Content Type ◽  
Argonaute 2 ◽  
Interfering Rna ◽  
Sirna Pathway

ABSTRACT Arboviruses are pathogens of humans and animals. A better understanding of the interactions between these pathogens and the arthropod vectors, such as mosquitoes, that transmit them is necessary to develop novel control measures. A major antiviral pathway in the mosquito vector is the exogenous small interfering RNA (exo-siRNA) pathway, which is induced by arbovirus-derived double-stranded RNA in infected cells. Although recent work has shown the key role played by Argonaute-2 (Ago-2) and Dicer-2 (Dcr-2) in this pathway, the regulatory mechanisms that govern these pathways have not been studied in mosquitoes. Here, we show that the Domino ortholog p400 has antiviral activity against the alphavirus Semliki Forest virus (Togaviridae) both in Aedes aegypti-derived cells and in vivo. Antiviral activity of p400 was also demonstrated against chikungunya virus (Togaviridae) and Bunyamwera virus (Peribunyaviridae) but not Zika virus (Flaviviridae). p400 was found to be expressed across mosquito tissues and regulated ago-2 but not dcr-2 transcript levels in A. aegypti mosquitoes. These findings provide novel insights into the regulation of an important aedine exo-siRNA pathway effector protein, Ago-2, by the Domino ortholog p400. They add functional insights to previous observations of this protein’s antiviral and RNA interference regulatory activities in Drosophila melanogaster. IMPORTANCE Female Aedes aegypti mosquitoes are vectors of human-infecting arthropod-borne viruses (arboviruses). In recent decades, the incidence of arthropod-borne viral infections has grown dramatically. Vector competence is influenced by many factors, including the mosquito’s antiviral defenses. The exogenous small interfering RNA (siRNA) pathway is a major antiviral response restricting arboviruses in mosquitoes. While the roles of the effectors of this pathway, Argonaute-2 and Dicer-2 are well characterized, nothing is known about its regulation in mosquitoes. In this study, we demonstrate that A. aegypti p400, whose ortholog Domino in Drosophila melanogaster is a chromatin-remodeling ATPase member of the Tip60 complex, regulates siRNA pathway activity and controls ago-2 expression levels. In addition, we found p400 to have antiviral activity against different arboviruses. Therefore, our study provides new insights into the regulation of the antiviral response in A. aegypti mosquitoes.

The phototoxicity of some 1,3-butadienes and related thiophenes against larvae of the mosquito Aedes aegypti and of the fruit fly Drosophila melanogaster

1983 ◽  
Vol 4 (04) ◽  
pp. 377-381 ◽  
Author(s):  
Jacques Kagan ◽  
Jean-Pierre Beny ◽  
Ging Chan ◽  
Som N. Dhawan ◽  
Jeffery A. Jaworski ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Aedes Aegypti ◽  
Fruit Fly
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