Analysis of oxygen-dependent cytokine expression in human mesenchymal stem cells derived from umbilical cord

2013 ◽  
Vol 353 (1) ◽  
pp. 117-122 ◽  
Author(s):  
Maren Lönne ◽  
Antonina Lavrentieva ◽  
Johanna-Gabriela Walter ◽  
Cornelia Kasper
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Human Mesenchymal Stem Cells ◽  
Cytokine Expression

scholarly journals Mechanisms of Action of Human Mesenchymal Stem Cells in Tissue Repair Regeneration and their Implications

2017 ◽  
Vol 53 (02) ◽  
pp. 104-120 ◽  
Author(s):  
Manisha Singh ◽  
Suchi Gupta ◽  
Sonali Rawat ◽  
Swati Midha ◽  
Krishan Gopal Jain ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Tissue Repair ◽  
Molecular Mechanisms ◽  
Human Mesenchymal Stem Cells ◽  
Mechanisms Of Action ◽  
Maximum Output ◽  
Cell Replacement Therapy ◽  
Tissue Repair And Regeneration

ABSTRACTCell replacement therapy holds a promising future in the treatment of degenerative diseases related to neuronal, cardiac and bone tissues. In such kind of diseases, there is a progressive loss of specific types of cells. Currently the most upcoming and trusted cell candidate is Mesenchymal Stem Cells (MSCs) as these cells are easy to isolate from the tissue, easy to maintain and expand and no ethical concerns are linked. MSCs can be obtained from a number of sources like bone marrow, umbilical cord blood, umbilical cord, dental pulp, adipose tissues, etc. MSCs help in tissue repair and regeneration by various mechanisms of action like cell differentiation, immunomodulation, paracrine effect, etc. The future of regenerative medicine lies in tissue engineering and exploiting various properties to yield maximum output. In the current review article, we have targeted the repair and regeneration mechanisms of MSCs in neurodegenerative diseases, cardiac diseases and those related to bones. Yet there is a lot to understand, discover and then understand again about the molecular mechanisms of MSCs and then applying this knowledge in developing the therapy to get maximum repair and regeneration of concerned tissue and in turn the recovery of the patient.

Human mesenchymal stem cells from the umbilical cord matrix: Successful isolation and ex vivo expansion using serum-/xeno-free culture media

2013 ◽  
Vol 8 (4) ◽  
pp. 448-458 ◽  
Author(s):  
Irina N. Simões ◽  
Joana S. Boura ◽  
Francisco dos Santos ◽  
Pedro Z. Andrade ◽  
Carla M. P. Cardoso ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Culture Media ◽  
Ex Vivo ◽  
Human Mesenchymal Stem Cells ◽  
Ex Vivo Expansion ◽  
Successful Isolation ◽  
Umbilical Cord Matrix

scholarly journals Adult bone marrow is a rich source of human mesenchymal ‘stem’ cells but umbilical cord and mobilized adult blood are not

2003 ◽  
Vol 121 (2) ◽  
pp. 368-374 ◽  
Author(s):  
Sarah A. Wexler ◽  
Craig Donaldson ◽  
Patricia Denning-Kendall ◽  
Claire Rice ◽  
Ben Bradley ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Human Mesenchymal Stem Cells ◽  
Rich Source ◽  
Adult Bone Marrow ◽  
Adult Bone

scholarly journals Baculovirus Transduction of Mesenchymal Stem Cells Triggers the Toll-Like Receptor 3 Pathway

2009 ◽  
Vol 83 (20) ◽  
pp. 10548-10556 ◽  
Author(s):  
Guan-Yu Chen ◽  
Hsiao-Chiao Shiah ◽  
Hung-Ju Su ◽  
Chi-Yuan Chen ◽  
Yung-Jen Chuang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Viral Vectors ◽  
Viral Vector ◽  
Interleukin 1 ◽  
Human Mesenchymal Stem Cells ◽  
Cytokine Expression ◽  
Toll Like Receptor ◽  
Safety Concerns ◽  
Surface Marker Expression

ABSTRACT Human mesenchymal stem cells (hMSCs) can be genetically modified with viral vectors and hold promise as a cell source for regenerative medicine, yet how hMSCs respond to viral vector transduction remains poorly understood, leaving the safety concerns unaddressed. Here, we explored the responses of hMSCs against an emerging DNA viral vector, baculovirus (BV), and discovered that BV transduction perturbed the transcription of 816 genes associated with five signaling pathways. Surprisingly, Toll-like receptor-3 (TLR3), a receptor that generally recognizes double-stranded RNA, was apparently upregulated by BV transduction, as confirmed by microarray, PCR array, flow cytometry, and confocal microscopy. Cytokine array data showed that BV transduction triggered robust secretion of interleukin-6 (IL-6) and IL-8 but not of other inflammatory cytokines and beta interferon (IFN-β). BV transduction activated the signaling molecules (e.g., Toll/interleukin-1 receptor domain-containing adaptor-inducing IFN-β, NF-κB, and IFN regulatory factor 3) downstream of TLR3, while silencing the TLR3 gene with small interfering RNA considerably abolished cytokine expression and promoted cell migration. These data demonstrate, for the first time, that a DNA viral vector can activate the TLR3 pathway in hMSCs and lead to a cytokine expression profile distinct from that in immune cells. These findings underscore the importance of evaluating whether the TLR3 signaling cascade plays roles in the immune response provoked by other DNA vectors (e.g., adenovirus). Nonetheless, BV transduction barely disturbed surface marker expression and induced only transient and mild cytokine responses, thereby easing the safety concerns of using BV for hMSCs engineering.

Co-culture of Umbilical Cord Blood CD34+Cells with Human Mesenchymal Stem Cells

2006 ◽  
Vol 0 (0) ◽  
pp. 060913044658049
Author(s):  
Yue Zhang ◽  
Chou Chai ◽  
Xue-Song Jiang ◽  
Swee-Hin Teoh ◽  
Kam W. Leong
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Human Mesenchymal Stem Cells ◽  
Cd34 Cells ◽  
Cord Blood Cd34

Inducing hepatogenic differentiation of human mesenchymal stem cells derived from umbilical cord blood

2014 ◽  
Vol 39 (1) ◽  
pp. 6
Author(s):  
DareenA Mohamed ◽  
MoustafaZ Moustafa ◽  
GhadaM Balah ◽  
EnasA Elzamarany ◽  
NahlaA Nosair
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Human Mesenchymal Stem Cells ◽  
Hepatogenic Differentiation

scholarly journals Comparative Analysis of Human Mesenchymal Stem Cells from Umbilical Cord, Dental Pulp, and Menstrual Blood as Sources for Cell Therapy

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Huaijuan Ren ◽  
Yunxia Sang ◽  
Fengli Zhang ◽  
Zhaoqing Liu ◽  
Nianmin Qi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factor ◽  
Cell Therapy ◽  
Umbilical Cord ◽  
Dental Pulp ◽  
Keratinocyte Growth Factor ◽  
Human Mesenchymal Stem Cells ◽  
Menstrual Blood ◽  
Tissue Repair And Regeneration

Although mesenchymal stem cells (MSCs) based therapy has been considered as a promising tool for tissue repair and regeneration, the optimal cell source remains unknown. Umbilical cord (UC), dental pulp (DP), and menstrual blood (MB) are easily accessible sources, which make them attractive candidates for MSCs. The goal of this study was to compare the biological characteristics, including morphology, proliferation, antiapoptosis, multilineage differentiation capacity, and immunophenotype of UC-, DP-, and MB-MSCs in order to provide a theoretical basis for clinical selection and application of these cells. As a result, all UC-, DP-, and MB-MSCs have self-renewal capacity and multipotentiality. However, the UC-MSCs seemed to have higher cell proliferation ability, while DP-MSCs may have significant advantages for osteogenic differentiation, lower cell apoptosis, and senescence. These differences may be associated with the different expression level of cytokines, including vascular endothelial growth factor, fibroblast growth factor, keratinocyte growth factor, and hepatocyte growth factor in each of the MSCs. Comprehensively, our results suggest DP-MSCs may be a desired source for clinical applications of cell therapy.

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