Effect of isolation methodology on stem cell properties and multilineage differentiation potential of human dental pulp stem cells

2013 ◽  
Vol 353 (1) ◽  
pp. 65-78 ◽  
Author(s):  
P. Hilkens ◽  
P. Gervois ◽  
Y. Fanton ◽  
J. Vanormelingen ◽  
W. Martens ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Multilineage Differentiation ◽  
Differentiation Potential ◽  
Human Dental Pulp

scholarly journals Multilineage Differentiation Potential of Human Dental Pulp Stem Cells—Impact of 3D and Hypoxic Environment on Osteogenesis In Vitro

2020 ◽  
Vol 21 (17) ◽  
pp. 6172
Author(s):  
Anna Labedz-Maslowska ◽  
Natalia Bryniarska ◽  
Andrzej Kubiak ◽  
Tomasz Kaczmarzyk ◽  
Malgorzata Sekula-Stryjewska ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Osteogenic Potential ◽  
Stem Cell Population ◽  
Differentiation Potential ◽  
Human Dental Pulp

Human dental pulp harbours unique stem cell population exhibiting mesenchymal stem/stromal cell (MSC) characteristics. This study aimed to analyse the differentiation potential and other essential functional and morphological features of dental pulp stem cells (DPSCs) in comparison with Wharton’s jelly-derived MSCs from the umbilical cord (UC-MSCs), and to evaluate the osteogenic differentiation of DPSCs in 3D culture with a hypoxic microenvironment resembling the stem cell niche. Human DPSCs as well as UC-MSCs were isolated from primary human tissues and were subjected to a series of experiments. We established a multiantigenic profile of DPSCs with CD45−/CD14−/CD34−/CD29+/CD44+/CD73+/CD90+/CD105+/Stro-1+/HLA-DR− (using flow cytometry) and confirmed their tri-lineage osteogenic, chondrogenic, and adipogenic differentiation potential (using qRT-PCR and histochemical staining) in comparison with the UC-MSCs. The results also demonstrated the potency of DPSCs to differentiate into osteoblasts in vitro. Moreover, we showed that the DPSCs exhibit limited cardiomyogenic and endothelial differentiation potential. Decreased proliferation and metabolic activity as well as increased osteogenic differentiation of DPSCs in vitro, attributed to 3D cell encapsulation and low oxygen concentration, were also observed. DPSCs exhibiting elevated osteogenic potential may serve as potential candidates for a cell-based product for advanced therapy, particularly for bone repair. Novel tissue engineering approaches combining DPSCs, 3D biomaterial scaffolds, and other stimulating chemical factors may represent innovative strategies for pro-regenerative therapies.

scholarly journals Multilineage Differentiation Potential of Stem Cells Derived from Human Dental Pulp after Cryopreservation

2006 ◽  
Vol 0 (0) ◽  
pp. 060928122958008 ◽  
Author(s):  
Weibo Zhang ◽  
X. Frank Walboomers ◽  
Songtao Shi ◽  
Mingwen Fan ◽  
John A. Jansen
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Multilineage Differentiation ◽  
Differentiation Potential ◽  
Human Dental Pulp

Comparison of two digestion strategies on characteristics and differentiation potential of human dental pulp stem cells

2018 ◽  
Vol 93 ◽  
pp. 74-79 ◽  
Author(s):  
Maziar Ebrahimi Dastgurdi ◽  
Fatemeh Ejeian ◽  
Marzie Nematollahi ◽  
Ahmad Motaghi ◽  
Mohammad Hossein Nasr-Esfahani
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Differentiation Potential ◽  
Human Dental Pulp

scholarly journals The Effect of Mesoporous Bioactive Glass Nanoparticles/Graphene Oxide Composites on the Differentiation and Mineralization of Human Dental Pulp Stem Cells

Nanomaterials ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 620 ◽  
Author(s):  
Jae Hwa Ahn ◽  
In-Ryoung Kim ◽  
Yeon Kim ◽  
Dong-Hyun Kim ◽  
Soo-Byung Park ◽  
...  
Keyword(s):  
Stem Cells ◽  
Graphene Oxide ◽  
Bioactive Glass ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Mrna Levels ◽  
Alizarin Red ◽  
Differentiation Potential ◽  
Human Dental Pulp ◽  
Mesoporous Bioactive Glass

The purpose of this study was to investigate the effects of mesoporous bioactive glass nanoparticle (MBN)/graphene oxide (GO) composites on the mineralization ability and differentiation potential of human dental pulp stem cells (hDPSCs). MBN/GO composites were synthesized using the sol-gel method and colloidal processing to enhance the bioactivity and mechanical properties of MBN. Characterization using FESEM, XRD, FTIR, and Raman spectrometry showed that the composites were successfully synthesized. hDPSCs were then cultured directly on the MBN/GO (40:1 and 20:1) composites in vitro. MBN/GO promoted the proliferation and alkaline phosphatase (ALP) activity of hDPSCs. In addition, qRT-PCR showed that MBN/GO regulated the mRNA levels of odontogenic markers (dentin sialophosphoprotein (DSPP), dentine matrix protein 1 (DMP-1), ALP, matrix extracellular phosphoglycoprotein (MEPE), bone morphogenetic protein 2 (BMP-2), and runt-related transcription factor 2 (RUNX-2)). The mRNA levels of DSPP and DMP-1, two odontogenesis-specific markers, were considerably upregulated in hDPSCs in response to growth on the MBN/GO composites. Western blot analysis revealed similar results. Alizarin red S staining was subsequently performed to further investigate MBN/GO-induced mineralization of hDPSCs. It was revealed that MBN/GO composites promote odontogenic differentiation via the Wnt/β-catenin signaling pathway. Collectively, the results of the present study suggest that MBN/GO composites may promote the differentiation of hDPSCs into odontoblast-like cells, and potentially induce dentin formation.

scholarly journals PD-1 is required to maintain stem cell properties in human dental pulp stem cells

2018 ◽  
Vol 25 (7) ◽  
pp. 1350-1360 ◽  
Author(s):  
Yao Liu ◽  
Huan Jing ◽  
Xiaoxing Kou ◽  
Chider Chen ◽  
Dawei Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp

Stem Cell Properties of Human Dental Pulp Stem Cells

2002 ◽  
Vol 81 (8) ◽  
pp. 531-535 ◽  
Author(s):  
S. Gronthos ◽  
J. Brahim ◽  
W. Li ◽  
L.W. Fisher ◽  
N. Cherman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp

scholarly journals Isolation of Human Dental Pulp Stem Cells and Their Characteristics Before and After Cryopreservation

2021 ◽  
Vol 31 (1) ◽  
pp. 58-69
Author(s):  
Svitlana Mazur ◽  
◽  
Olena Rogulska ◽  
Olena Revenko ◽  
Nataliya Volkova ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Third Molar ◽  
Tooth Germ ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Dental Pulp Stem Cells ◽  
Slow Cooling ◽  
Differentiation Potential ◽  
Before And After ◽  
Human Dental Pulp

Dental pulp stem cells (DPSCs) from human third molar tooth germ (wisdom tooth) were isolated using a collagenasebased enzymatic method, the obtained cells were analyzed as for morphology in monolayer culture, immunophenotype, proliferation and differentiation potential before and after cryopreservation. In this study, we showed that based on morphological features, surface markers profile and differentiation potential, the isolated DPSCs corresponded to multipotent mesenchymal stromal cells. DPSCs cryopreservation by slow cooling (1 °С / min) down to –80°C with subsequent immersion into liquid nitrogen in cryoprotectant-free culture medium led to cell death. Cryopreservation using the same protocol in the presence of 10% dimethyl sulfoxide (DMSO) and 20% serum ensured (82 ± 6)% cell viability; while metabolic and proliferative activity, as well as the ability to differentiate into the osteo- and adipogenic lineages of cryopreserved DPSCs were similar to their non-cryopreserved counterparts.

scholarly journals Effects of Helioxanthin Derivative-Treated Human Dental Pulp Stem Cells on Fracture Healing

2020 ◽  
Vol 21 (23) ◽  
pp. 9158
Author(s):  
Daiki Yamakawa ◽  
Yoko Kawase-Koga ◽  
Yasuyuki Fujii ◽  
Yuki Kanno ◽  
Marika Sato ◽  
...  
Keyword(s):  
Stem Cells ◽  
Fracture Healing ◽  
Efficient Method ◽  
Dental Pulp ◽  
Fracture Site ◽  
Dental Pulp Stem Cells ◽  
Differentiation Potential ◽  
Normal Medium ◽  
Human Dental Pulp

Bone defects affect patients functionally and psychologically and can decrease quality of life. To resolve these problems, a simple and efficient method of bone regeneration is required. Human dental pulp stem cells (DPSCs) have high proliferative ability and multilineage differentiation potential. In our previous study, we reported a highly efficient method to induce osteogenic differentiation using DPSC sheets treated with a helioxanthin derivative (4-(4-methoxyphenyl)pyrido[40,30:4,5]thieno[2,3-b]pyridine-2-carboxamide (TH)) in a mouse calvarial defect model. However, the localization of the DPSCs after transplantation remains unknown. Therefore, in this study, we investigated the localization of transplanted DPSCs in a mouse fracture model. DPSCs were collected from six healthy patients aged 18–29 years, cultured in normal medium (NM), osteogenic medium (OM), or OM with TH, and fabricated them into cell sheets. To evaluate the efficacy of fracture healing using DPSCs treated with OM+TH, and to clarify the localization of the transplanted DPSC sheets in vivo, we transplanted OM+TH-treated DPSC sheets labeled with PKH26 into mouse tibiae fractures. We demonstrated that transplanted OM+TH-treated DPSCs sheets were localized to the fracture site and facilitated bone formation. These results indicated that transplanted OM+TH-treated DPSCs were localized at fracture sites and directly promoted fracture healing.

Sign in / Sign up

Export Citation Format

Share Document