Establishment of an immortalized cell line derived from the pupal ovary of Mythimna separata (Lepidoptera: Noctuidae) and identification of the cell source

2021 ◽  
Author(s):  
Yan Tong ◽  
Lu-Qiang Cheng ◽  
Xuan Li ◽  
Xu-Peng Yu ◽  
Rui-Hao Shu ◽  
...  
Keyword(s):  
Cell Line ◽  
Mythimna Separata ◽  
Immortalized Cell Line ◽  
Cell Source ◽  
Immortalized Cell ◽  
Lepidoptera Noctuidae

scholarly journals Isolation of Primary Mouse Pulmonary Microvascular Endothelial Cells and Generation of an Immortalized Cell Line to Obtain Sufficient Extracellular Vesicles

2021 ◽  
Vol 12 ◽  
Author(s):  
Xu Liu ◽  
Feiping Xia ◽  
Xiao Wu ◽  
Ying Tang ◽  
Lu Wang ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Cell Line ◽  
Extracellular Vesicles ◽  
Phenotypic Expression ◽  
Microvascular Endothelial Cells ◽  
Pulmonary Microvascular Endothelial Cells ◽  
Immortalized Cell Line ◽  
Primary Mouse ◽  
Microvascular Endothelial ◽  
Immortalized Cell

Pulmonary microvascular endothelial cells (PMECs) and the extracellular vesicles (EVs) derived from PMECs participate in maintaining pulmonary homeostasis and mediating the inflammatory response. However, obtaining a high-purity population of PMECs and their EVs from mouse is still notoriously difficult. Herein we provide a method to isolate primary mouse PMECs (pMPMECs) and to transduce SV40 lentivirus into pMPMECs to establish an immortalized cell line (iMPMECs), which provides sufficient quantities of EVs for further studies. pMPMECs and iMPMECs can be identified using morphologic criteria, a phenotypic expression profile (e.g., CD31, CD144, G. simplicifolia lectin binding), and functional properties (e.g., Dil-acetylated low-density protein uptake, Matrigel angiogenesis). Furthermore, pMPMEC–EVs and iMPMEC–EVs can be identified and compared. The characteristics of pMPMEC–EVs and iMPMEC–EVs are ascertained by transmission electron microscopy, nanoparticle tracking analysis, and specific protein markers. iMPMECs produce far more EVs than pMPMECs, while their particle size distribution is similar. Our detailed protocol to isolate and immortalize MPMECs will provide researchers with an in vitro model to investigate the specific roles of EVs in pulmonary physiology and diseases.

scholarly journals FRI0516 CHONDROGENIC EFFECT OF KARTOGENIN ON AN IMMORTALIZED CELL LINE DERIVED FROM MESENCHYMAL STROMAL CELLS ISOLATED FROM HUMAN BONE MARROW

2019 ◽  
Author(s):  
Carolina Guiance-Varela ◽  
Cristina Rodríguez-Pereira ◽  
Elena Fernandez-Burguera ◽  
Tamara Hermida Gómez ◽  
Noa Goyanes ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Line ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Immortalized Cell Line ◽  
Immortalized Cell

scholarly journals Chondrogenic effect of kartogenin on an immortalized cell line derived from mesenchymal stromal cells isolated from human bone marrow

2019 ◽  
Vol 27 ◽  
pp. S435-S436
Author(s):  
C. Guiance-Varela ◽  
C. Rodríguez-Pereira ◽  
E.F. Burguera ◽  
T. Hermida-Gómez ◽  
N. Goyanes ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Line ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Immortalized Cell Line ◽  
Immortalized Cell

An immortalized cell line expresses properties of activated microglial cells

1992 ◽  
Vol 31 (4) ◽  
pp. 616-621 ◽  
Author(s):  
V. Bocchini ◽  
R. Mazzolla ◽  
R. Barluzzi ◽  
E. Blasi ◽  
P. Sick ◽  
...  
Keyword(s):  
Cell Line ◽  
Microglial Cells ◽  
Immortalized Cell Line ◽  
Immortalized Cell
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