Enhancement of adenosine production by Bacillus subtilis CGMCC 4484 through metabolic flux analysis and simplified feeding strategies

2013 ◽  
Vol 36 (12) ◽  
pp. 1851-1859 ◽  
Author(s):  
Xiaochun Chen ◽  
Chunwei Zhang ◽  
Jian Cheng ◽  
Xinchi Shi ◽  
Lei Li ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Feeding Strategies ◽  
Flux Analysis

13C-metabolic flux analysis in heterologous cellulase production by Bacillus subtilis genome-reduced strain

2014 ◽  
Vol 179 ◽  
pp. 42-49 ◽  
Author(s):  
Yoshihiro Toya ◽  
Takashi Hirasawa ◽  
Takuya Morimoto ◽  
Kenta Masuda ◽  
Yasushi Kageyama ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Cellulase Production ◽  
Flux Analysis ◽  
13C Metabolic Flux Analysis ◽  
Subtilis Genome

Combining metabolic flux analysis and adaptive evolution to enhance lipase production in Bacillus subtilis

2019 ◽  
Vol 46 (8) ◽  
pp. 1091-1101 ◽  
Author(s):  
Kai Yuan ◽  
Ping Song ◽  
Shuang Li ◽  
Song Gao ◽  
Jianping Wen ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Adaptive Evolution ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Lipase Production ◽  
Flux Analysis

De novo engineering and metabolic flux analysis of inosine biosynthesis in Bacillus subtilis

2011 ◽  
Vol 33 (8) ◽  
pp. 1575-1580 ◽  
Author(s):  
Haojian Li ◽  
Guoqiang Zhang ◽  
Aihua Deng ◽  
Ning Chen ◽  
Tingyi Wen
Keyword(s):  
Bacillus Subtilis ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
De Novo ◽  
Flux Analysis

Two-stage oxygen supply strategy for enhanced lipase production by Bacillus subtilis based on metabolic flux analysis

2013 ◽  
Vol 71 ◽  
pp. 1-10 ◽  
Author(s):  
Ping Song ◽  
Chen Chen ◽  
Qianqian Tian ◽  
Ming Lin ◽  
He Huang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Metabolic Flux Analysis ◽  
Oxygen Supply ◽  
Metabolic Flux ◽  
Lipase Production ◽  
Flux Analysis ◽  
Two Stage

scholarly journals Bacillus subtilis Metabolism and Energetics in Carbon-Limited and Excess-Carbon Chemostat Culture

2001 ◽  
Vol 183 (24) ◽  
pp. 7308-7317 ◽  
Author(s):  
Michael Dauner ◽  
Tazio Storni ◽  
Uwe Sauer
Keyword(s):  
Bacillus Subtilis ◽  
Total Energy ◽  
Energy Flux ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Tca Cycle ◽  
Overflow Metabolism ◽  
Flux Analysis ◽  
Total Energy Flux ◽  
Excess Glucose

ABSTRACT The energetic efficiency of microbial growth is significantly reduced in cultures growing under glucose excess compared to cultures growing under glucose limitation, but the magnitude to which different energy-dissipating processes contribute to the reduced efficiency is currently not well understood. We introduce here a new concept for balancing the total cellular energy flux that is based on the conversion of energy and carbon fluxes into energy equivalents, and we apply this concept to glucose-, ammonia-, and phosphate-limited chemostat cultures of riboflavin-producing Bacillus subtilis. Based on [U-13C6]glucose-labeling experiments and metabolic flux analysis, the total energy flux in slow-growing, glucose-limited B. subtilis is almost exclusively partitioned in maintenance metabolism and biomass formation. In excess-glucose cultures, in contrast, uncoupling of anabolism and catabolism is primarily achieved by overflow metabolism, while two quantified futile enzyme cycles and metabolic shifts to energetically less efficient pathways are negligible. In most cultures, about 20% of the total energy flux could not be assigned to a particular energy-consuming process and thus are probably dissipated by processes such as ion leakage that are not being considered at present. In contrast to glucose- or ammonia-limited cultures, metabolic flux analysis revealed low tricarboxylic acid (TCA) cycle fluxes in phosphate-limited B. subtilis, which is consistent with CcpA-dependent catabolite repression of the cycle and/or transcriptional activation of genes involved in overflow metabolism in the presence of excess glucose. ATP-dependent control of in vivo enzyme activity appears to be irrelevant for the observed differences in TCA cycle fluxes.

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