Improving the catalytic potential and substrate tolerance of Gibberella intermedia nitrilase by whole-cell immobilization

2014 ◽  
Vol 38 (1) ◽  
pp. 189-197 ◽  
Author(s):  
Heng Li ◽  
Tao Yang ◽  
Jin-Song Gong ◽  
Lei Xiong ◽  
Zhen-Ming Lu ◽  
...  
Keyword(s):  
Cell Immobilization ◽  
Whole Cell ◽  
Catalytic Potential ◽  
Substrate Tolerance ◽  
Gibberella Intermedia

scholarly journals Tuning a bi-enzymatic cascade reaction in Escherichia coli to facilitate NADPH regeneration for ε-caprolactone production

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Jinghui Xiong ◽  
Hefeng Chen ◽  
Ran Liu ◽  
Hao Yu ◽  
Min Zhuo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Binding Sites ◽  
Regeneration System ◽  
Nadph Regeneration ◽  
Whole Cell ◽  
Cyclohexanone Monooxygenase ◽  
Ribosome Binding ◽  
Ribosome Binding Sites ◽  
Whole Cell Biocatalyst ◽  
Substrate Tolerance

Abstractε-Caprolactone is a monomer of poly(ε-caprolactone) which has been widely used in tissue engineering due to its biodegradability and biocompatibility. To meet the massive demand for this monomer, an efficient whole-cell biocatalytic approach was constructed to boost the ε-caprolactone production using cyclohexanol as substrate. Combining an alcohol dehydrogenase (ADH) with a cyclohexanone monooxygenase (CHMO) in Escherichia coli, a self-sufficient NADPH-cofactor regeneration system was obtained. Furthermore, some improved variants with the better substrate tolerance and higher catalytic ability to ε-caprolactone production were designed by regulating the ribosome binding sites. The best mutant strain exhibited an ε-caprolactone yield of 0.80 mol/mol using 60 mM cyclohexanol as substrate, while the starting strain only got a conversion of 0.38 mol/mol when 20 mM cyclohexanol was supplemented. The engineered whole-cell biocatalyst was used in four sequential batches to achieve a production of 126 mM ε-caprolactone with a high molar yield of 0.78 mol/mol.

Enhancing catalytic stability and cadaverine tolerance by whole-cell immobilization and the addition of cell protectant during cadaverine production

2018 ◽  
Vol 102 (18) ◽  
pp. 7837-7847 ◽  
Author(s):  
Guoguang Wei ◽  
Weichao Ma ◽  
Alei Zhang ◽  
Xun Cao ◽  
Jinshan Shen ◽  
...  
Keyword(s):  
Cell Immobilization ◽  
Whole Cell ◽  
Catalytic Stability

Ethanol production by whole cell immobilization using lignocellulosic materials as solid matrix

1993 ◽  
Vol 75 (2) ◽  
pp. 132-137 ◽  
Author(s):  
Debabrata Das ◽  
Nandkishor R. Gaidhani ◽  
Krishna Murari ◽  
Paritosh Sen Gupta
Keyword(s):  
Ethanol Production ◽  
Cell Immobilization ◽  
Solid Matrix ◽  
Lignocellulosic Materials ◽  
Whole Cell
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