Use of solvent mixtures for total lipid extraction of Chlorella vulgaris and gas chromatography FAME analysis

2017 ◽  
Vol 40 (9) ◽  
pp. 1363-1373 ◽  
Author(s):  
Narges Moradi-kheibari ◽  
Hossein Ahmadzadeh ◽  
Majid Hosseini
Keyword(s):  
Gas Chromatography ◽  
Total Lipid ◽  
Chlorella Vulgaris ◽  
Lipid Extraction ◽  
Solvent Mixtures ◽  
Total Lipid Extraction ◽  
Fame Analysis

scholarly journals Comparison between several methods of total lipid extraction from Chlorella vulgaris biomass

2015 ◽  
Vol 22 ◽  
pp. 95-99 ◽  
Author(s):  
Raquel Rezende dos Santos ◽  
Daniel Mendonça Moreira ◽  
Claudete Norie Kunigami ◽  
Donato Alexandre Gomes Aranda ◽  
Cláudia Maria Luz Lapa Teixeira
Keyword(s):  
Total Lipid ◽  
Chlorella Vulgaris ◽  
Lipid Extraction ◽  
Total Lipid Extraction

scholarly journals The BUME method: a novel automated chloroform-free 96-well total lipid extraction method for blood plasma

2012 ◽  
Vol 53 (8) ◽  
pp. 1690-1700 ◽  
Author(s):  
Lars Löfgren ◽  
Marcus Ståhlman ◽  
Gun-Britt Forsberg ◽  
Sinikka Saarinen ◽  
Ralf Nilsson ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Total Lipid ◽  
Extraction Method ◽  
Lipid Extraction ◽  
Total Lipid Extraction

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

1959 ◽  
Vol 37 (1) ◽  
pp. 911-917 ◽  
Author(s):  
E. G. Bligh ◽  
W. J. Dyer
Keyword(s):  
Rapid Method ◽  
Total Lipid ◽  
Lipid Extraction ◽  
Fish Muscle ◽  
Lipid Extract ◽  
Total Lipid Extraction ◽  
Extraction And Purification ◽  
Chloroform Layer ◽  
Frozen Fish ◽  
Entire Procedure

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

Differentiation Between Natural Hydrocarbons and Low Level Diesel Oil Contamination in Cooked Lobster Meat

1975 ◽  
Vol 32 (2) ◽  
pp. 316-320 ◽  
Author(s):  
M. Paradis ◽  
R. G. Ackman
Keyword(s):  
Gas Chromatography ◽  
Column Chromatography ◽  
Lipid Extraction ◽  
Diesel Oil ◽  
Extraction Column ◽  
Oil Contamination ◽  
Low Level ◽  
Total Lipid Extraction ◽  
Temperature Programmed ◽  
Normal Background

A combination of total lipid extraction, column chromatography, and temperature-programmed gas chromatography was required to demonstrate disputed low level diesel oil contamination in cooked lobster meat. A specific diesel oil contaminant was indicated but identifiable components were not greatly in excess of the same compounds which were also found to be a normal background in organoleptically acceptable canned lobster meat.

scholarly journals Rapid Quantification of Yeast Lipid using Microwave-Assisted Total Lipid Extraction and HPLC-CAD

2013 ◽  
Vol 85 (10) ◽  
pp. 4912-4919 ◽  
Author(s):  
Sakda Khoomrung ◽  
Pramote Chumnanpuen ◽  
Suwanee Jansa-Ard ◽  
Marcus Ståhlman ◽  
Intawat Nookaew ◽  
...  
Keyword(s):  
Total Lipid ◽  
Lipid Extraction ◽  
Total Lipid Extraction ◽  
Microwave Assisted ◽  
Rapid Quantification ◽  
Yeast Lipid

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

1959 ◽  
Vol 37 (8) ◽  
pp. 911-917 ◽  
Author(s):  
E. G. Bligh ◽  
W. J. Dyer
Keyword(s):  
Rapid Method ◽  
Total Lipid ◽  
Lipid Extraction ◽  
Fish Muscle ◽  
Lipid Extract ◽  
Total Lipid Extraction ◽  
Extraction And Purification ◽  
Chloroform Layer ◽  
Frozen Fish ◽  
Entire Procedure

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

Sign in / Sign up

Export Citation Format

Share Document