scholarly journals The BUME method: a novel automated chloroform-free 96-well total lipid extraction method for blood plasma

2012 ◽  
Vol 53 (8) ◽  
pp. 1690-1700 ◽  
Author(s):  
Lars Löfgren ◽  
Marcus Ståhlman ◽  
Gun-Britt Forsberg ◽  
Sinikka Saarinen ◽  
Ralf Nilsson ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Total Lipid ◽  
Extraction Method ◽  
Lipid Extraction ◽  
Total Lipid Extraction

scholarly journals Evaluation of the results of the QUASIMEME lipid intercomparison: the Bligh & Dyer total lipid extraction method

1996 ◽  
Vol 32 (8-9) ◽  
pp. 674-680 ◽  
Author(s):  
Patrick Roose ◽  
Foppe Smedes
Keyword(s):  
Total Lipid ◽  
Extraction Method ◽  
Lipid Extraction ◽  
Total Lipid Extraction

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

1959 ◽  
Vol 37 (1) ◽  
pp. 911-917 ◽  
Author(s):  
E. G. Bligh ◽  
W. J. Dyer
Keyword(s):  
Rapid Method ◽  
Total Lipid ◽  
Lipid Extraction ◽  
Fish Muscle ◽  
Lipid Extract ◽  
Total Lipid Extraction ◽  
Extraction And Purification ◽  
Chloroform Layer ◽  
Frozen Fish ◽  
Entire Procedure

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

scholarly journals Comparison between several methods of total lipid extraction from Chlorella vulgaris biomass

2015 ◽  
Vol 22 ◽  
pp. 95-99 ◽  
Author(s):  
Raquel Rezende dos Santos ◽  
Daniel Mendonça Moreira ◽  
Claudete Norie Kunigami ◽  
Donato Alexandre Gomes Aranda ◽  
Cláudia Maria Luz Lapa Teixeira
Keyword(s):  
Total Lipid ◽  
Chlorella Vulgaris ◽  
Lipid Extraction ◽  
Total Lipid Extraction

scholarly journals The BUME method: a new rapid and simple chloroform-free method for total lipid extraction of animal tissue

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Lars Löfgren ◽  
Gun-Britt Forsberg ◽  
Marcus Ståhlman
Keyword(s):  
Total Lipid ◽  
Lipid Extraction ◽  
Animal Tissue ◽  
Total Lipid Extraction

Use of solvent mixtures for total lipid extraction of Chlorella vulgaris and gas chromatography FAME analysis

2017 ◽  
Vol 40 (9) ◽  
pp. 1363-1373 ◽  
Author(s):  
Narges Moradi-kheibari ◽  
Hossein Ahmadzadeh ◽  
Majid Hosseini
Keyword(s):  
Gas Chromatography ◽  
Total Lipid ◽  
Chlorella Vulgaris ◽  
Lipid Extraction ◽  
Solvent Mixtures ◽  
Total Lipid Extraction ◽  
Fame Analysis

scholarly journals Optimization of a novel lipid extraction process from microalgae

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xiaojie Ren ◽  
Chao Wei ◽  
Qi Yan ◽  
Xin Shan ◽  
Mengyun Wu ◽  
...  
Keyword(s):  
Water Treatment ◽  
Organic Solvents ◽  
Total Lipid ◽  
Extraction Method ◽  
Treatment Time ◽  
Lipid Extraction ◽  
Dry Weight ◽  
Extraction Solvent ◽  
Lipid Yield ◽  
Chloroform Methanol

AbstractPrevious study found that the solvent extraction efficiency of lipid in microalgae could be greatly improved by washing algae cells before the second time extraction. Based on the "organic solvents–water–organic solvents" method, this research further studied the effect of four solvent systems (acetone, chloroform/methanol, chloroform/methanol/water, dichloromethane/methanol), two types of water treatment (vortex and ultrasonic), three water treatment time gradient (0 s, 30 s, 120 s) on the lipid extraction at three different microalgae growth stages (3rd day, 5th day, 9th day). The results show that the combination of water treatment type, treatment time and solvent is very important to the efficiency of lipid extraction. The total lipid extracted was generally increased by 10–30% after water treatment. Especially under the condition of 120 s vortex water treatment with dichloromethane/methanol as extraction solvent, the total lipid extracted increased by 61.14%. In addition, microalgae cells at different culture stages had different sensitivity to water treatment. In this study, under the combination of chloroform/methanol/water as extraction solvent and vortex water treatment for 120 s, the highest lipid yield was obtained on the ninth day of cell culture, which accounts 47.88% of the cell dry weight (478 mg/g cell dry weight). The changes of cell morphology and structure after water treatment were studied by scanning electron microscope, and it was found that water treatment could seriously destroy the cell membrane damaged by solvent, thus promoting the release of lipids. This study further optimizes the "solvent–water–solvent" lipid extraction method, which neither produces impurities nor damages the lipid quality, and can reduce the amount of organic solvent applied in the classical lipid extraction method with the same lipid yield, so it has a broad application prospect.

scholarly journals Rapid Quantification of Yeast Lipid using Microwave-Assisted Total Lipid Extraction and HPLC-CAD

2013 ◽  
Vol 85 (10) ◽  
pp. 4912-4919 ◽  
Author(s):  
Sakda Khoomrung ◽  
Pramote Chumnanpuen ◽  
Suwanee Jansa-Ard ◽  
Marcus Ståhlman ◽  
Intawat Nookaew ◽  
...  
Keyword(s):  
Total Lipid ◽  
Lipid Extraction ◽  
Total Lipid Extraction ◽  
Microwave Assisted ◽  
Rapid Quantification ◽  
Yeast Lipid

Total Lipid Extraction of Homogenized and Intact Lean Fish Muscles Using Pressurized Fluid Extraction and Batch Extraction Techniques

2005 ◽  
Vol 53 (14) ◽  
pp. 5506-5512 ◽  
Author(s):  
Giorgis Isaac ◽  
Monica Waldebäck ◽  
Ulla Eriksson ◽  
Göran Odham ◽  
Karin E. Markides
Keyword(s):  
Total Lipid ◽  
Lipid Extraction ◽  
Extraction Techniques ◽  
Fluid Extraction ◽  
Batch Extraction ◽  
Total Lipid Extraction ◽  
Fish Muscles ◽  
Lean Fish ◽  
Pressurized Fluid Extraction

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

1959 ◽  
Vol 37 (8) ◽  
pp. 911-917 ◽  
Author(s):  
E. G. Bligh ◽  
W. J. Dyer
Keyword(s):  
Rapid Method ◽  
Total Lipid ◽  
Lipid Extraction ◽  
Fish Muscle ◽  
Lipid Extract ◽  
Total Lipid Extraction ◽  
Extraction And Purification ◽  
Chloroform Layer ◽  
Frozen Fish ◽  
Entire Procedure

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

scholarly journals Total Lipid Extraction

2011 ◽  
Author(s):  
Wei Zou ◽  
Wei Zou
Keyword(s):  
Total Lipid ◽  
Lipid Extraction ◽  
Total Lipid Extraction
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