A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

1959 ◽  
Vol 37 (8) ◽  
pp. 911-917 ◽  
Author(s):  
E. G. Bligh ◽  
W. J. Dyer
Keyword(s):  
Rapid Method ◽  
Total Lipid ◽  
Lipid Extraction ◽  
Fish Muscle ◽  
Lipid Extract ◽  
Total Lipid Extraction ◽  
Extraction And Purification ◽  
Chloroform Layer ◽  
Frozen Fish ◽  
Entire Procedure

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

1959 ◽  
Vol 37 (1) ◽  
pp. 911-917 ◽  
Author(s):  
E. G. Bligh ◽  
W. J. Dyer
Keyword(s):  
Rapid Method ◽  
Total Lipid ◽  
Lipid Extraction ◽  
Fish Muscle ◽  
Lipid Extract ◽  
Total Lipid Extraction ◽  
Extraction And Purification ◽  
Chloroform Layer ◽  
Frozen Fish ◽  
Entire Procedure

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

scholarly journals The BUME method: a novel automated chloroform-free 96-well total lipid extraction method for blood plasma

2012 ◽  
Vol 53 (8) ◽  
pp. 1690-1700 ◽  
Author(s):  
Lars Löfgren ◽  
Marcus Ståhlman ◽  
Gun-Britt Forsberg ◽  
Sinikka Saarinen ◽  
Ralf Nilsson ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Total Lipid ◽  
Extraction Method ◽  
Lipid Extraction ◽  
Total Lipid Extraction

scholarly journals Comparison between several methods of total lipid extraction from Chlorella vulgaris biomass

2015 ◽  
Vol 22 ◽  
pp. 95-99 ◽  
Author(s):  
Raquel Rezende dos Santos ◽  
Daniel Mendonça Moreira ◽  
Claudete Norie Kunigami ◽  
Donato Alexandre Gomes Aranda ◽  
Cláudia Maria Luz Lapa Teixeira
Keyword(s):  
Total Lipid ◽  
Chlorella Vulgaris ◽  
Lipid Extraction ◽  
Total Lipid Extraction

scholarly journals Rapid Quantification of Yeast Lipid using Microwave-Assisted Total Lipid Extraction and HPLC-CAD

2013 ◽  
Vol 85 (10) ◽  
pp. 4912-4919 ◽  
Author(s):  
Sakda Khoomrung ◽  
Pramote Chumnanpuen ◽  
Suwanee Jansa-Ard ◽  
Marcus Ståhlman ◽  
Intawat Nookaew ◽  
...  
Keyword(s):  
Total Lipid ◽  
Lipid Extraction ◽  
Total Lipid Extraction ◽  
Microwave Assisted ◽  
Rapid Quantification ◽  
Yeast Lipid

Semimicro Method for Determining Total Lipids in Fish Meal

1969 ◽  
Vol 52 (4) ◽  
pp. 688-692
Author(s):  
Mary E Ambrose ◽  
Barbara J Roche ◽  
George M Knobl
Keyword(s):  
Rapid Method ◽  
Extraction Method ◽  
Fish Meal ◽  
Total Lipids ◽  
Chloroform Layer ◽  
Aoac Method ◽  
Entire Procedure

Abstract A simple and rapid method lias been developed for determining lipids in fish meal. Lipids are extracted with chloroform and methanol; the entire procedure requires no more than 10 min. An aliquot of the chloroform layer is dried at 50°C under nitrogen and then equilibrated 90 min in a desiccator. Twelve samples can be completed in a single day, compared to the three days required to complete an analysis by AOAC method 22.037. Results of the extraction method average 99.0% of those obtained by method 22.037.

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