A continuous membrane-less anaerobic fluidized bed microbial fuel cell was used to investigate the effects of fluidization parameters on the electro-genesis capacity of the microbial fuel cell. The experimental results indicated that active carbon particles could significantly decrease the start-up time and increase the output voltage of the fluidized bed microbial fuel cell. At steady state operating conditions, the microbial fuel cell output voltages were 700~900 mV and 600~700 mV for with and without the active carbon particles, respectively. The fluidization behavior of the active carbon particles in the microbial fuel cell reactor is one of the key parameters that influences the generation of electricity. The experimental results indicated that to obtain the optimal electric production performance, an appropriate flow velocity should be determined. The fluidized bed microbial fuel cell could be operated in large-scale wastewater treatment processes with high Chemical Oxygen Demand removal efficiencies that reached 93%.