Regulatory volume decrease in northern fur seal (Callorhinus ursinus) red blood cells

2006 ◽  
Vol 16 (1) ◽  
pp. 61-63
Author(s):  
H. Ochiai ◽  
N. Hishiyama ◽  
K. Higa ◽  
K. Koyama ◽  
M. Seita ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Regulatory Volume Decrease ◽  
Callorhinus Ursinus ◽  
Northern Fur Seal ◽  
Fur Seal ◽  
Volume Decrease

K(+)-Cl- cotransport and volume regulation in the light and the dense fraction of high-K+ dog red blood cells

1997 ◽  
Vol 273 (3) ◽  
pp. R991-R998 ◽  
Author(s):  
H. Fujise ◽  
K. Abe ◽  
M. Kamimura ◽  
H. Ochiai
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Divalent Cations ◽  
Regulatory Volume Decrease ◽  
Marker Enzyme ◽  
High K ◽  
Dense Fraction ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Volume Decrease ◽  
Light Cells

We examined a chloride (Cl-)-dependent K+ transport (K(+)-Cl- cotransport) and regulatory volume decrease in dog red blood cells with high K+, low Na+, and high glutathione (GSH) content (HK/HG) due to the presence of an Na(+)-K+ pump. The HK/HG cells were separated according to their density, and the age-marker enzyme activities, such as glucose-6-phosphate dehydrogenase and cholinesterase, were determined. Unexpectedly, we found that young cells were heavier (more dense) and smaller in size compared with the old cells, which were lighter (less dense) and larger. The K(+)-Cl- cotransport was nearly 10-fold higher in the most dense cells, representing a 12% fraction of the total population compared with the lightest cohort. Although K(+)-Cl- cotransport in both the dense and the light cells was activated by N-ethylmaleimide, swelling and depletion of cellular divalent cations and the activation of the transport in the dense cells was greater. Both the dense and light cells regulated their volume when they were isosmotically swollen. Therefore, the lower activity of K(+)-Cl- cotransport might not explain the relative large volume in old HK/HG cells. The concentration of GSH and glutamate was higher in the light cells. Thus the higher the GSH and glutamate concentration, the greater the cell volume and the lower the K(+)-Cl- cotransport.

Potassium conductance activated during regulatory volume decrease by mudpuppy red blood cells

1996 ◽  
Vol 270 (4) ◽  
pp. R801-R810
Author(s):  
L. J. Bergeron ◽  
A. J. Stever ◽  
D. B. Light
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Enhanced Recovery ◽  
Regulatory Volume Decrease ◽  
Cell Swelling ◽  
K Channel ◽  
Ionophore A23187 ◽  
Patch Clamp Technique ◽  
Extracellular Medium ◽  
Volume Decrease

The cellular basis of regulatory volume decrease (RVD) by mudpuppy (Necturus maculosus) red blood cells (RBCs) was examined. Volume regulation was inhibited by replacing extracellular Na+ with K+. In contrast, addition of gramicidin (5 microM) to the extracellular medium enhanced RVD. The K(+)-channel blocker quinine (1 mM) also inhibited RVD, and this inhibition was reversed by gramicidin (5 microM). In addition, a 0 Ca(2+)-EGTA Ringer blocked RVD, whereas the Ca2+ ionophore A23187 ( microM) enhanced recovery of cell volume. The stretch-activated ion channel antagonist gadolinium (10 microM) inhibited RVD, and this effect was reversed by A23187 (2 microM). Furthermore, the calmodulin inhibitors pimozide (10 microM) and N-(6-aminohexyl)-5-chloro-1-napthalene-sulfonamide (0.1 mM) blocked RVD, and this inhibition was reversed with gramicidin (5 microM). Consistent with these findings, a K(+)-selective membrane conductance was activated by exposing RBCs to a 0.5x Ringer solution (observed with the whole cell patch clamp technique). This conductance was inhibited by quinine (1 mM), gadolinium (10 microM), and pimozide (10 microM). These results indicate that cell swelling activates a K+ conductance by a Ca(2+)-calmodulin-dependent mechanism and that this channel mediates K+ loss during RVD.

scholarly journals Regulatory volume decrease in carp red blood cells: mechanisms and oxygenation-dependency of volume-activated potassium and amino acid transport

1995 ◽  
Vol 198 (1) ◽  
pp. 155-165 ◽  
Author(s):  
F Jensen
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Regulatory Volume Decrease ◽  
Cell Swelling ◽  
Organic Osmolytes ◽  
Osmotic Swelling ◽  
Original Cell ◽  
Sufficient Stimulus ◽  
K Release ◽  
Volume Decrease

Hypo-osmotic swelling of carp red blood cells (RBCs) induced a regulatory volume decrease (RVD), which restored the original cell volume within 140 min in oxygenated RBCs, whereas volume recovery was incomplete in deoxygenated RBCs. The complete RVD in oxygenated RBCs resulted from a sustained volume-activated release of K+, Cl- and amino acids (AAs). In the absence of ouabain, the cells also lost Na+ as released K+ was partially regained via the Na+/K+ pump. Inorganic osmolytes contributed approximately 70 %, and organic osmolytes approximately 30 %, to the RVD of oxygenated RBCs. Oxygenation in isotonic medium per se activated a K+ efflux from the RBCs. Hypo-osmotic cell swelling stimulated an additional K+ release. The oxygenation-activated and the volume-activated K+ efflux were both inhibited by DIDS and by the replacement of Cl- with NO3-, showing that both types of K+ efflux were Cl--dependent and probably occurred via the same K+/Cl- cotransport mechanism. Once activated by oxygenation, the K+/Cl- cotransport was further stimulated by cell swelling. Deoxygenation inactivated the oxygenation-induced Cl--dependent K+ release and cell swelling was not a sufficient stimulus to reactivate it significantly. In deoxygenated RBCs, the volume-induced K+ release was transient and primarily Cl--independent and, in the absence of ouabain, the cell K+ content recovered towards control values via the Na+/K+ pump. The Cl--independent K+ efflux seemed to involve K+/H+ exchange, but other transport routes also participated. Swelling-activated AA release differed in kinetics between oxygenated and deoxygenated RBCs but was important for RVD at both oxygenation degrees. Approximately 70 % of the AA release was inhibited by DIDS and substitution of NO3- for Cl- produced a 50 % inhibition, suggesting that the AA permeation was partly Cl--dependent. In oxygenated RBCs, a reduction in pH lowered the volume-activated Cl--dependent K+ efflux but not the AA efflux. In deoxygenated RBCs, the acute volume-stimulated K+ and AA release were both increased by acidification. The data are discussed in relation to possible transducer mechanisms and physiological implications.

Hypoxia inhibits the regulatory volume decrease in red blood cells of common frog ( Rana temporaria )

2018 ◽  
Vol 219-220 ◽  
pp. 44-47 ◽  
Author(s):  
Aleksandra Y. Andreyeva ◽  
Elizaveta A. Skverchinskaya ◽  
Stepan Gambaryan ◽  
Aleksander A. Soldatov ◽  
Igor V. Mindukshev
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Rana Temporaria ◽  
Regulatory Volume Decrease ◽  
Common Frog ◽  
Frog Rana Temporaria ◽  
Volume Decrease

Protein Kinase C and Regulatory Volume Decrease in Mudpuppy Red Blood Cells

1998 ◽  
Vol 166 (2) ◽  
pp. 119-132 ◽  
Author(s):  
D.B. Light ◽  
M.R. Adler ◽  
J.K. Ter Beest ◽  
S.A. Botsford ◽  
R.T. Gronau
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Regulatory Volume Decrease ◽  
Kinase C ◽  
Volume Decrease

GSH depletion, K-Cl cotransport, and regulatory volume decrease in high-K/high-GSH dog red blood cells

2001 ◽  
Vol 281 (6) ◽  
pp. C2003-C2009 ◽  
Author(s):  
Hiroshi Fujise ◽  
Kazunari Higa ◽  
Tomomi Kanemaru ◽  
Miwa Fukuda ◽  
Norma C. Adragna ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Regulatory Volume Decrease ◽  
Cell Volume Regulation ◽  
Redox System ◽  
Glutathione S Transferase ◽  
High Potassium ◽  
High K ◽  
Protein Thiols ◽  
Volume Decrease

Thiol reagents activate K-Cl cotransport (K-Cl COT), the Cl-dependent and Na-independent ouabain-resistant K flux, in red blood cells (RBCs) of several species, upon depletion of cellular glutathione (GSH). K-Cl COT is physiologically active in high potassium (HK), high GSH (HG) dog RBCs. In this unique model, we studied whether the same inverse relationship exists between GSH levels and K-Cl COT activity found in other species. The effects of GSH depletion by three different chemical reactions [nitrite (NO2)-mediated oxidation, diazene dicarboxylic acid bis- N, N-dimethylamide (diamide)-induced dithiol formation, and glutathione S-transferase (GST)-catalyzed conjugation of GSH with 1-chloro-2,4-dinitrobenzene (CDNB)] were tested on K-Cl COT and regulatory volume decrease (RVD). After 85% GSH depletion, all three interventions stimulated K-Cl COT half-maximally with the following order of potency: diamide > NO2 > CDNB. Repletion of GSH reversed K-Cl COT stimulation by 50%. Cl-dependent RVD accompanied K-Cl COT activation by NO2 and diamide. K-Cl COT activation at concentration ratios of oxidant/GSH greater than unity was irreversible, suggesting either nitrosothiolation, heterodithiol formation, or GST-mediated dinitrophenylation of protein thiols. The data support the hypothesis that an intact redox system, rather than the absolute GSH levels, protects K-Cl COT activity and cell volume regulation from thiol modification.

Stable-isotope ratios of blood components from captive northern fur seals (Callorhinus ursinus) and their diet: applications for studying the foraging ecology of wild otariids

2002 ◽  
Vol 80 (5) ◽  
pp. 902-909 ◽  
Author(s):  
Carolyn M Kurle
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Foraging Ecology ◽  
Isotope Ratios ◽  
Clupea Harengus ◽  
Callorhinus Ursinus ◽  
Blood Components ◽  
Fur Seals ◽  
Fur Seal ◽  
Northern Fur Seals

Stable nitrogen and carbon isotope ratios (13C/12C and 15N/14N, respectively) of plasma, serum, clotted red blood cells, and unclotted red blood cells from six captive northern fur seals (Callorhinus ursinus) and two fish species in their diet, Atlantic herring (Clupea harengus) and Icelandic capelin (Mallotus villosus), were measured. The δ15N values from the fur seal blood components ranged from 15.5 ± 0.1‰ (mean ± SE; all RBCs) to 16.7 ± 0.1‰ (plasma), and δ13C values ranged from –18.3 ± 0.1‰ (serum) to –17.5 ± 0.1‰ (clotted RBCs). Fur seal blood components had higher δ15N and δ13C values than their diet. Mean enrichments of 15N between fur seal RBCs and plasma/serum and their prey were +4.1 and +5.2‰, respectively, while mean 13C enrichments were +0.6‰ (serum), +1.0‰ (plasma), and +1.3 to +1.4‰ (all RBCs). Fur seal blood components did not differ in δ15N or δ13C between the sexes. One female (Baabs) was pregnant at the time of sampling and was resampled 4 months later, when she was approximately 3 months post parturient. The δ15N values of the blood components taken from Baabs during pregnancy were all higher by 0.6‰ (unclotted RBCs) to 1.3‰ (plasma) than those of samples taken during lactation, while her δ13C values were nearly the same (RBCs) for the two time periods or lower during lactation by 0.5‰ (serum) and 0.7‰ (plasma). This study was the first to analyze isotope ratios of blood components from captive fur seals and their prey. The subsequent establishment of fractionation values between captive fur seals and their diet can be used to interpret trophic level and habitat usage of otariids feeding in the wild.

scholarly journals Timing of Implantation in the Northern Fur Seal, Callorhinus ursinus

1997 ◽  
Vol 78 (2) ◽  
pp. 675-683 ◽  
Author(s):  
V. B. Scheffer ◽  
A. E. York
Keyword(s):  
Callorhinus Ursinus ◽  
Northern Fur Seal ◽  
Fur Seal
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