Prey composition impacts lipid and protein digestibility in northern fur seals (Callorhinus ursinus)

Pinnipeds have specific macronutrient (protein, lipid) requirements to satisfy physiological functions, yet little is known about how diet characteristics affect macronutrient digestibility. We measured relative and absolute lipid and protein digestibility in six female northern fur seals (Callorhinus ursinus (Linnaeus, 1758)) fed eight experimental diets composed variously of four prey species (Pacific herring, Clupea pallasii Valenciennes in Cuvier and Valenciennes, 1847; walleye pollock, Gadus chalcogrammus Pallas, 1814 (formerly Theragra chalcogramma (Pallas, 1814)); capelin, Mallotus villosus (Müller, 1776); magister armhook squid, Berryteuthis magister (Berry, 1913)). We quantified how digestibility was affected by proximate composition of the diet (% lipid or % protein), levels of food mass and macronutrient intake, and tested for any potential benefit of multi-species diets. Overall, digestibility of both protein and lipid were high across diets, although macronutrient retention of lipids (96.0%–98.4%) was significantly higher than protein (95.7%–96.7%) for all but the two highest protein diets. Increased levels of protein intake resulted in increased protein retention, but decreased lipid digestibility. There was no evidence that mixed-species diets provide greater macronutrient digestibility over single-species diets. The results suggest that high to moderate lipid diets are more beneficial to northern fur seals because they lead to increased levels of lipid retention without large decreases in protein digestibility. This raises concerns that dietary factors may be contributing to the population declines of northern fur seals in the Bering Sea.

Stress-related and reproductive hormones in hair from three north Pacific otariid species: Steller sea lions, California sea lions and northern fur seals

Assessing the physiological impact of stressors in pinnipeds is logistically challenging, and many hormones are altered by capture and handling, limiting the utility of metabolically active tissues. Hair is increasingly being used to investigate stress-related and reproductive hormones in wildlife populations due to less-invasive collection methods, being metabolically inert once grown and containing multiple biomarkers of ecological interest. We validated enzyme immunoassays for measuring aldosterone, cortisol, corticosterone, and testosterone in lanugo (natal hair grown in utero) samples collected from Steller sea lions (Eumetopias jubatus), California sea lions (Zalophus californianus), and northern fur seals (Callorhinus ursinus). We applied laboratory validation methods including recovery of added mass, parallelism and dilution linearity. We found no effects due to differences in alcohol- versus detergent-based cleaning methods. Further, there were no significant differences in hormone concentrations in hair samples collected immediately after the molt and the subsequent samples collected over 1 year, indicating steroid hormones are stable once deposited into pinniped hair. We found no sex differences in any hormone concentrations, likely due to the lanugo being grown in utero and influenced by maternal hormone concentrations. For Steller sea lion and California sea lion pups, we found hormone concentrations significantly differed between rookeries, which warrants future research. Hair provides a novel tissue to explore the intrinsic or extrinsic drivers behind hormone measurements in otariids, which can be paired with multiple health-related metrics to further investigate possible drivers of physiological stress.